Data/hora: 13/07/2025 10:42:36

Biblioteca(s): 	Embrapa Agroindústria de Alimentos; Embrapa Agroindústria Tropical.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 1	
Data corrente: 	01/10/2020	
Data da última atualização: 	02/10/2020	
Autoria: 	SANTOS, K. P. dos; SILVA, C. M.; BRIGIDA, A. I. S.; GONÇALVES, L. R. B.	
Afiliação: 	Kimberle Paiva dos Santos, Universidade Federal do Ceará; CAROLINE MELLINGER SILVA, CTAA; ANA IRAIDY SANTA BRIGIDA, CNPAT; Luciana Rocha Barros Gonçalves, Universidade Federal do Ceará.	
Título: 	Modifying alcalase activity and stability by immobilization onto chitosan aiming at the production of bioactive peptides by hydrolysis of tilapia skin gelatin.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Process biochemistry, v. 97, p. 27-36, 2020.	
DOI: 	https://doi.org/10.1016/j.procbio.2020.06.019	
Idioma: 	Inglês	
Conteúdo: 	The protease from Bacillus licheniformis, commercially known as Alcalase (R), was insolubilized and stabilized by immobilization onto activated chitosan. Activation with different agents, such as glutaraldehyde (GLU-Chi), glyoxyl (GLY-Chi) and divinyl sulfone (DVS-Chi) was investigated. The effect of the immobilization protocol, for instance different pH and times, were also evaluated. GLU-Chi showed the highest activity (35.6UNPA/g) with the smallest substrate (N-Boc-L-alanine p-nitrophenyl-ester, NPA), while GLY-Chi showed the highest activity (1.5 UAzocasein/g) using the greatest substrate (azocasein). A 24-h immobilization period was enough to stabilize the enzyme using the three supports under almost all conditions. Operational stability in azocasein hydrolysis was assayed and GLU-Chi showed no activity loss during 5 cycles. DVS-Chi retained around 70 % of its initial activity after the fifth cycle, whereas GLY-Chi activity retained only 10 %. Finally, the biocatalysts were used in the hydrolysis of tilapia skin gelatin aiming the production of peptides with antioxidant activity. The protein hy-drolysates obtained using GLU-Chi presented the highest antioxidant activity (36.7 mu M Trolox Eq). However, the best results of operational stability were obtained using DVS-Chi, which did not lose its initial activity after 3 consecutive cycles of gelatin hydrolysis.	
Palavras-Chave: 	Alcalase; Immobilization; Skin gelatin; Stability.	
Thesagro: 	Tilápia.	
Thesaurus Nal: 	Chitosan; Food technology; Protein hydrolysates.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroindústria de Alimentos (CTAA)	
Exemplares: 	CTAA - (15026-1) - UPC - AP - DD -  -  -  - DIS
CNPAT - (16501-1) - UPC - AP - DD -  -  - ART20078 - DIS