|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
26/02/1996 |
Data da última atualização: |
23/06/2023 |
Autoria: |
STEWART, D. J.; McKERN, N. M.; RAMSHAW, J. A. M.; TULLOCH, P. A. |
Título: |
The effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot. |
Ano de publicação: |
1991 |
Fonte/Imprenta: |
Veterinary Microbiology, v. 27, n. 3/4, p. 283-293, May, 1991. |
DOI: |
10.1016/0378-1135(91)90155-9 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili. |
Palavras-Chave: |
Foot Rot; Pododermatite. |
Thesagro: |
Doença; Ovino. |
Thesaurus Nal: |
Bacterial antigens; Bacteroides; Circular dichroism spectroscopy; Disease control; Disease prevention; Gel chromatography; Glucosides; Immunology; Polysorbates; Sheep diseases; Ultrastructure; Vaccination. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02435naa a2200361 a 4500 001 1522917 005 2023-06-23 008 1991 bl uuuu u00u1 u #d 024 7 $a10.1016/0378-1135(91)90155-9$2DOI 100 1 $aSTEWART, D. J. 245 $aThe effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot.$h[electronic resource] 260 $c1991 520 $aAbstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili. 650 $aBacterial antigens 650 $aBacteroides 650 $aCircular dichroism spectroscopy 650 $aDisease control 650 $aDisease prevention 650 $aGel chromatography 650 $aGlucosides 650 $aImmunology 650 $aPolysorbates 650 $aSheep diseases 650 $aUltrastructure 650 $aVaccination 650 $aDoença 650 $aOvino 653 $aFoot Rot 653 $aPododermatite 700 1 $aMcKERN, N. M. 700 1 $aRAMSHAW, J. A. M. 700 1 $aTULLOCH, P. A. 773 $tVeterinary Microbiology$gv. 27, n. 3/4, p. 283-293, May, 1991.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 15 | |
1. |  | ALLEBRANDT, R.; MARCON FILHO, J. L.; WUZ, D. A.; PEREIRA DE BEM, B.; KRETZSCHMAR, A. A.; RUFATO, L. Pruning methods on the yield performance and oenological potential of 'Nebbiolo' grapevine. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n, 11, p. 1017-1022, novembro 2017. Título em português: Métodos de poda sobre o desempenho produtivo e potencial enológico da uva 'Nebbiolo'.Biblioteca(s): Embrapa Unidades Centrais. |
|    |
2. |  | BEM, B. P. de; BAGO, A.; ALLEBRANDT, R.; MARCON FILHO, J. L.; WURZ, D. A. Botrytis bunch rot on 'Sauvignon Blanc' grapevine on the Y-trellis and vertical shoot-positioned training systems. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 9, p. 818-822, ago. 2017. Título em português: Podridão cinzenta em videira 'Sauvignon Blanc' sob sistemas de sustentação ípsilon e espaldeira.Biblioteca(s): Embrapa Unidades Centrais. |
|    |
5. |  | MARCON FILHO, J. L.; KRETZSCHMAR, A. A.; HIPOLITO, JH. de S.; RUFATO, A. de R.; RUFATO, L.; WURZ, D. A. Increasing the length of EM-9 interstock enhances production efficiency in Imperial Gala apples. Revista Ceres, v. 66, n. 3, p. 178-183, 2019Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Uva e Vinho. |
|    |
6. |  | MARCON FILHO, J. L.; KRETZSCHMAR, A. A.; RUFATO, L.; SILVA, L. C. da; MADEIRA, F. C.; RIBEIRO, R. S.; RUFATO, A. de R. Evaluation of the productive and vegetative aspects of the cultivar 'Imperial Gala' apple tree with EM-9 interstem in different lengths. Acta Horticulturae, The Hague, n. 872, p. 375-378, ago. 2010. 375-378 Trabalho apresentado no VIII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics, Florianópolis, 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Uva e Vinho. |
|    |
11. |  | SILVA, L. C. da; RUFATO, L.; KRETZSCHMAR, A. A.; MARCON FILHO, J. L. Raleio de cachos em vinhedos de altitude e qualidade do vinho da cultivar Syrah. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 2, p. 148-154, fev. 2009. Título em inglês: Cluster thinning in high altitude vineyards and wine quality from the cultivar Syrah.Biblioteca(s): Embrapa Unidades Centrais. |
|    |
12. |  | WURZ, D. A.; BRIGHENTI, A. F.; MARCON FILHO, J. L.; ALLEBRANDT, R.; BEM, B. P. de; RUFATO, L.; KRETZSCHMAR, A. A. Agronomic performance of 'Cabernet Sauvignon' with leaf removal management in a high-altitude region of Southern Brazil. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 10, p. 869-876, out. 2017. Título em português: Desempenho agronômico de 'Cabernet Sauvignon' com manejo de desfolha em região de elevada altitude no Sul do Brasil.Biblioteca(s): Embrapa Unidades Centrais. |
|    |
13. |  | WURZ, D. A.; MARCON FILHO, J. L.; BRIGHENTI, A. F.; ALLEBRANDT, R.; BEM, B. P. de; MAGRO, M.; RUFATO, L.; KRETZSCHMAR, A. A. Effect of shoot topping intensity on 'Cabernet Franc' grapevine maturity in high-altitude region. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 10, p. 946-950, out. 2017. Notas Científicas. Título em português: Efeito de intensidade de desponte na maturação de 'Cabernet Franc' cultivada em região de altitude.Biblioteca(s): Embrapa Unidades Centrais. |
|    |
15. |  | ZALAMENA, J.; CASSOL, P. C.; BRUNETTO, G.; PANISSON, J.; MARCON FILHO, J.; SCHLEMPER, C. Produtividade e composição de uva e de vinho de videiras consorciadas com plantas de cobertura. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 2, p. 182-189, fev. 2013. Título em inglês: Productivity and composition of grapes and wine of vines intercropped with cover crops.Biblioteca(s): Embrapa Unidades Centrais. |
|    |
Registros recuperados : 15 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|