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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
11/07/2018 |
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Data da última atualização: |
11/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMALHO, S. R.; BEZERRA, C. da S.; LIMA, L. S.; MARIA NETO, S.; OLIVEIRA, C. F. R. de; VERBISCK, N. V.; MACEDO, M. L. R. |
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Afiliação: |
Suellen Rodrigues Ramalho; Cezar da Silva Bezerra; Letícia Souza Lima; Simone Maria Neto; Caio Fernando Ramalho de Oliveira; NEWTON VALERIO VERBISCK, CNPGC; Maria Lígia Rodrigues Macedo. |
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Título: |
Novel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal Agricultural Food Chemistry. v. 66, n. 6, p. 1349-1358, fev. 2018. |
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Idioma: |
Português |
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Conteúdo: |
A novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control. |
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Palavras-Chave: |
Kunitz-type inhibitor; Pest insect. |
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Thesaurus Nal: |
Fabaceae; Trypsin inhibitors. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01706naa a2200241 a 4500
001 2093186
005 2018-07-11
008 2018 bl uuuu u00u1 u #d
100 1 $aRAMALHO, S. R.
245 $aNovel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae.$h[electronic resource]
260 $c2018
520 $aA novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control.
650 $aFabaceae
650 $aTrypsin inhibitors
653 $aKunitz-type inhibitor
653 $aPest insect
700 1 $aBEZERRA, C. da S.
700 1 $aLIMA, L. S.
700 1 $aMARIA NETO, S.
700 1 $aOLIVEIRA, C. F. R. de
700 1 $aVERBISCK, N. V.
700 1 $aMACEDO, M. L. R.
773 $tJournal Agricultural Food Chemistry.$gv. 66, n. 6, p. 1349-1358, fev. 2018.
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Embrapa Gado de Corte (CNPGC) |
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