| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
26/02/1996 |
|
Data da última atualização: |
23/06/2023 |
|
Autoria: |
STEWART, D. J.; McKERN, N. M.; RAMSHAW, J. A. M.; TULLOCH, P. A. |
|
Título: |
The effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot. |
|
Ano de publicação: |
1991 |
|
Fonte/Imprenta: |
Veterinary Microbiology, v. 27, n. 3/4, p. 283-293, May, 1991. |
|
DOI: |
10.1016/0378-1135(91)90155-9 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili. |
|
Palavras-Chave: |
Foot Rot; Pododermatite. |
|
Thesagro: |
Doença; Ovino. |
|
Thesaurus Nal: |
Bacterial antigens; Bacteroides; Circular dichroism spectroscopy; Disease control; Disease prevention; Gel chromatography; Glucosides; Immunology; Polysorbates; Sheep diseases; Ultrastructure; Vaccination. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02435naa a2200361 a 4500
001 1522917
005 2023-06-23
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1016/0378-1135(91)90155-9$2DOI
100 1 $aSTEWART, D. J.
245 $aThe effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot.$h[electronic resource]
260 $c1991
520 $aAbstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili.
650 $aBacterial antigens
650 $aBacteroides
650 $aCircular dichroism spectroscopy
650 $aDisease control
650 $aDisease prevention
650 $aGel chromatography
650 $aGlucosides
650 $aImmunology
650 $aPolysorbates
650 $aSheep diseases
650 $aUltrastructure
650 $aVaccination
650 $aDoença
650 $aOvino
653 $aFoot Rot
653 $aPododermatite
700 1 $aMcKERN, N. M.
700 1 $aRAMSHAW, J. A. M.
700 1 $aTULLOCH, P. A.
773 $tVeterinary Microbiology$gv. 27, n. 3/4, p. 283-293, May, 1991.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 6 | |
| 1. |  | SILVA, W. F. M. da; RUSCHEL, A. R.; BENTES, D. S.; BARROS, H. da C. Avaliação demográfica e dinâmica da ucuúba (Virola michelli Heckel) em uma área explorada há 30 anos na Flona do tapajós. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA, 14., 2010, Belém, PA. Bolsista de iniciação científica: um aporte ao desenvolvimento da pesquisa agropecuária: anais. Belém, PA: Embrapa Amazônia Oriental, 2010. 1 CD-ROM. PIBIC 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 3. | | SILVA, W. F. M.; MAZZEI, L.; RUSCHEL, A. R.; SOARES, M. H. M.; BENTES, D. S.; GOUVEIA, D. M. Dynamics of the diameter distribuition of a logged forest in Brazilian amazon. In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 49., 2012, Bonito. Ecology, evolution and sustainable use of tropical biodiversity. [S.l.]: ATBC, 2012. p. 993. MAZZEI [i.e. FREITAS], L. J. M.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 4. | | SILVA, W. F. M. da; RUSCHEL, A. R.; BARROS, H. C. da C.; BENTES, D. S.; FEUILLEBOIS, M. do S. M.; SOUSA, J. L. de. Avaliação das espécies arbóreas dominantes na área florestal explorada há 30 anos na Floresta Nacional do Tapajós-PA. In: REUNIÃO ANUAL DA SBPC, 62., 2010, Natal. Ciências do mar: herança para o futuro: anais/resumos. [S.l.]: SBPC, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 5. | | REIS, L. P.; RUSCHEL, A. R.; BARROS, H. C. da C.; SILVA, W. F. M. da; BENTES, D. S.; SOUSA, J. L. de. Avaliação da dinâmica em 26 anos anos das 10 espécies madeireiras mais exploradas em área da Floresta Nacional do Tapajós-PA. In: REUNIÃO ANUAL DA SBPC, 62., 2010, Natal. Ciências do mar: herança para o futuro: anais/resumos. [S.l.]: SBPC, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 6. | | BENTES, D. S.; RUSCHEL, A. R.; SILVA, W. F. M. da; REIS, L. P.; BARROS, H. C. da C.; SOUSA, J. L. de. Estoque da floresta aos 30 anos após colheita de madeira na área de pesquisa do Km 67 na Floresta Nacional do Tapajós-PA. In: REUNIÃO ANUAL DA SBPC, 62., 2010, Natal. Ciências do mar: herança para o futuro: anais/resumos. [S.l.]: SBPC, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| Registros recuperados : 6 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
