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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/05/2009 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. |
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Afiliação: |
NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA/USP; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA/USP; TATIANA ALMEIDA DRUMOND TETZNER, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009. |
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DOI: |
10.1089/clo.2008.0044 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. MenosThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst ... Mostrar Tudo |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02377naa a2200205 a 4500
001 1048984
005 2023-03-15
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1089/clo.2008.0044$2DOI
100 1 $aSARAIVA, N. Z.
245 $aDemecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes.$h[electronic resource]
260 $c2009
520 $aThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine.
650 $aoocytes
700 1 $aPERECIN, F.
700 1 $aMÉO, S. C.
700 1 $aFERREIRA, C. R.
700 1 $aTETZNER, T. A. D.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells$gv. 11, n. 1, p. 141-152, mar. 2009.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 13 | |
| 3. |  | ALMEIDA, C. M. V. C. de; LOCATELLI, M.; LIMA, A. de A.; XAVIER, P. I.; CIDIN, A. C. M. Diversidade de espécies arbóreas e potencial madeireiro em sistemas agrossilviculturais com cacaueiro em Ouro Preto do Oeste, Rondônia, Brasil. Agrotrópica, v. 21, n.1, p. 73-82, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Rondônia. |
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| 5. | | SILVA, E. F. da; XAVIER, P. A. M.; CARVALHO FILHO, A. de; MOTTA, P. E. F. da; BACA, J. F. M. Avaliação da aptidão agrícola das terras das áreas desmatadas da Amazônia Legal para Lavouras no nível tecnológico médio. In: ENCONTRO REGIONAL DE CIÊNCIA DO SOLO NA AMAZÔNIA ORIENTAL, 2., 2016, Capanema, PA. Anais... Capanema, PA: UFRA, 2016. Amazon Soil 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 6. | | XAVIER, P. A. M.; SILVA, E. F. da; CARVALHO FILHO, A. de; MOTTA, P. E. F. da; BACA, J. F. M. Avaliação e caracterização da aptidão agrícola em áreas desmatadas do Estado de Rondônia para lavouras no nível tecnológico C. In: ENCONTRO REGIONAL DE CIÊNCIA DO SOLO NA AMAZÔNIA ORIENTAL, 2., 2016, Capanema, PA. Anais... Capanema, PA: UFRA, 2016. Amazon Soil 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 7. | | LIMA, M. V. D. O.; GUERRA, C. C.; LIRA, M. M. P.; XAVIER, P. R.; ARNAUD, A. M.; AMORIM, F. de M. Características das uvas do Vale do São Francisco sob o ponto de vista enológico. In: WORKSHOP INTERNACIONAL DE PESQUISA, 1., 2004, Recife e Petrolina. A produção de vinhos em regiões tropicais: anais. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 177-184. (Embrapa Uva e Vinho. Documentos, 60).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | CHAGAS, C. da S.; CARVALHO JUNIOR, W. de; PINHEIRO, H. S. K.; XAVIER, P. A. M.; BHERING, S. B.; PEREIRA, N. R.; CALDERANO FILHO, B. Mapping soil cation exchange capacity in a semiarid region through predictive models and covariates from remote sensing data. Revista Brasileira de Ciência do Solo, v. 42, article e0170183, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Solos. |
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| 9. | | XAVIER, P. C. N.; CHANG, M. R.; PAULA, C. R.; MATSUMOTO, F. E.; ASENSI, M. D.; MATOS, M. de F. C.; ANDREOTTI, R. Molecular characterization of Candida spp. isolates from patients with bloodstream infections. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba. MG, v. 46, n.6 nov./dez. 2013. p.786-787.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | PINHEIRO, H. S. K.; XAVIER, P. A. M.; ANJOS, L. H. C. dos; CHAGAS, C. da S.; CARVALHO JUNIOR, W. de. Pedometric tools applied to zoning management of areas in brazilian semiarid region. In: MOUDRY, J.; BERNAS, J.; MENDES, K.; SOUSA, R. N. de; TEIXEIRA, R. da S. (Ed.). Multifunctionality and impacts of organic agriculture. London: IntechOpen, 2019.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
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| 12. | | XAVIER, P. A. M.; ANJOS, L. H. C. dos; PINHEIRO, H. S. K.; CHAGAS, C. da S.; CARVALHO JUNIOR, W. de. Usage of pedometrics for data evaluation and harmonization in soil profiles from Cerrado region, Mato Grosso do Sul. In: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: proceedings... Viçosa, MG: SBCS, 2019. v. 2, p. 75. WCSS 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 13. | | SOUZA-SANTOS, Y. J.; ARGENTO, M. B. V.; FACHINATTO, W. M.; XAVIER, P. L. P.; ROCHETTI, A. L.; LOURENÇO, C. M.; CARVALHO, R. A.; FUKUMASU, H.; COLNAGO, L. A.; VANIN, F. M. Modulation of physicochemical, digestibility, and cytotoxic properties of pupunha (Bactris gasipaes) flour by different processes. Food Research International, v. 209, 116159, 2025. 15 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 13 | |
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