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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/09/2025 |
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Data da última atualização: |
17/09/2025 |
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Autoria: |
FARIA, O. A. C.; KUSSANO, N. R.; MOURA, A. O.; FARIA, L. C.; MARTINS, L. P.; SPRÍCIGO, J. F. W.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
O. A. C. FARIA, UNIVERSITY OF BRASILIA; A. O. MOURA, FEDERAL UNIVERSITY OF UBERLÂNDIA; L. C. FARIA, UNIVERSITY OF BRASILIA; L. P. MARTINS, UNIVERSITY OF BRASILIA; J. F. W. SPRÍCIGO, FEDERAL UNIVERSITY OF GOIÁS; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
Physiological oocyte maturation via intrafollicular transfer improves metabolic and molecular quality of bovine embryos. |
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Ano de publicação: |
2026 |
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Fonte/Imprenta: |
Theriogenology, v. 249, 117642, 2026. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2025.117642 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro maturation (IVM) has been widely used in bovine embryo production but is often associated with increased lipid accumulation and reduced cryotolerance. This study evaluated the intrafollicular transfer of immature oocytes (IFIOT) as an alternative oocyte maturation strategy, comparing it with conventional IVM in terms of embryo development, lipid metabolism, gene expression, cryosurvival, and DNA methylation. After maturation, oocytes were fertilized and cultured in vitro. Embryos were assessed for cleavage and blastocyst rates, lipid and mitochondrial content, total cell number, and survival after cryopreservation. Expanded blastocysts at Day 7 were transferred to synchronized recipients, and Day 14 embryos were collected for gene expression and methylation analyses. Cleavage and blastocyst rates were similar between groups (P > 0.05), but lipid content was significantly higher in IVM embryos (P < 0.05). Expression of PPARγ, a key transcription factor regulating lipid metabolism, was significantly higher in IVM-derived embryos (P < 0.0001), suggesting a more unbalanced metabolic profile compared to IFIOT. Mitochondrial activity and cell number did not differ. Overall cryosurvival was comparable; however, Day 8 IVM embryos showed reduced post-thaw survival relative to IFIOT (P < 0.05). DNA methylation levels in Satellite I and α-Satellite regions remained low in both groups, with no significant differences. These findings indicate that IFIOT supports oocyte maturation under more physiological conditions, leading to embryos with improved metabolic balance, reduced lipid accumulation, and enhanced cryotolerance. IFIOT may represent a valuable tool to optimize oocyte quality and increase the robustness of bovine in vitro embryo production systems. MenosIn vitro maturation (IVM) has been widely used in bovine embryo production but is often associated with increased lipid accumulation and reduced cryotolerance. This study evaluated the intrafollicular transfer of immature oocytes (IFIOT) as an alternative oocyte maturation strategy, comparing it with conventional IVM in terms of embryo development, lipid metabolism, gene expression, cryosurvival, and DNA methylation. After maturation, oocytes were fertilized and cultured in vitro. Embryos were assessed for cleavage and blastocyst rates, lipid and mitochondrial content, total cell number, and survival after cryopreservation. Expanded blastocysts at Day 7 were transferred to synchronized recipients, and Day 14 embryos were collected for gene expression and methylation analyses. Cleavage and blastocyst rates were similar between groups (P > 0.05), but lipid content was significantly higher in IVM embryos (P < 0.05). Expression of PPARγ, a key transcription factor regulating lipid metabolism, was significantly higher in IVM-derived embryos (P < 0.0001), suggesting a more unbalanced metabolic profile compared to IFIOT. Mitochondrial activity and cell number did not differ. Overall cryosurvival was comparable; however, Day 8 IVM embryos showed reduced post-thaw survival relative to IFIOT (P < 0.05). DNA methylation levels in Satellite I and α-Satellite regions remained low in both groups, with no significant differences. These findings indicate that IFIOT supports oocyte maturatio... Mostrar Tudo |
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Palavras-Chave: |
D14 embryo; In vitro embryo production. |
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Thesaurus Nal: |
DNA methylation; Gene expression; Lipid metabolism. |
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Categoria do assunto: |
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Marc: |
LEADER 02656naa a2200277 a 4500
001 2178880
005 2025-09-17
008 2026 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2025.117642$2DOI
100 1 $aFARIA, O. A. C.
245 $aPhysiological oocyte maturation via intrafollicular transfer improves metabolic and molecular quality of bovine embryos.$h[electronic resource]
260 $c2026
520 $aIn vitro maturation (IVM) has been widely used in bovine embryo production but is often associated with increased lipid accumulation and reduced cryotolerance. This study evaluated the intrafollicular transfer of immature oocytes (IFIOT) as an alternative oocyte maturation strategy, comparing it with conventional IVM in terms of embryo development, lipid metabolism, gene expression, cryosurvival, and DNA methylation. After maturation, oocytes were fertilized and cultured in vitro. Embryos were assessed for cleavage and blastocyst rates, lipid and mitochondrial content, total cell number, and survival after cryopreservation. Expanded blastocysts at Day 7 were transferred to synchronized recipients, and Day 14 embryos were collected for gene expression and methylation analyses. Cleavage and blastocyst rates were similar between groups (P > 0.05), but lipid content was significantly higher in IVM embryos (P < 0.05). Expression of PPARγ, a key transcription factor regulating lipid metabolism, was significantly higher in IVM-derived embryos (P < 0.0001), suggesting a more unbalanced metabolic profile compared to IFIOT. Mitochondrial activity and cell number did not differ. Overall cryosurvival was comparable; however, Day 8 IVM embryos showed reduced post-thaw survival relative to IFIOT (P < 0.05). DNA methylation levels in Satellite I and α-Satellite regions remained low in both groups, with no significant differences. These findings indicate that IFIOT supports oocyte maturation under more physiological conditions, leading to embryos with improved metabolic balance, reduced lipid accumulation, and enhanced cryotolerance. IFIOT may represent a valuable tool to optimize oocyte quality and increase the robustness of bovine in vitro embryo production systems.
650 $aDNA methylation
650 $aGene expression
650 $aLipid metabolism
653 $aD14 embryo
653 $aIn vitro embryo production
700 1 $aKUSSANO, N. R.
700 1 $aMOURA, A. O.
700 1 $aFARIA, L. C.
700 1 $aMARTINS, L. P.
700 1 $aSPRÍCIGO, J. F. W.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tTheriogenology$gv. 249, 117642, 2026.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 19 | |
| 5. |  | UEOKA, F. M.; ROMANO, M. R.; BORGES, F.; SETTER, C.; WINK, C. Métodos para superação da dormência em sementes de taxibranco (Tachigali vulgaris L.F Gomes da Silva E H.C Lima). IN: Semana Acadêmica - Sinop/2014, 1., 2014,Sinop, MT. Resumos... I Semana Acadêmica ? Sinop/2014, III Jornada Científica da Embrapa Agrossilvipastoril, Seminário Integrador PIBID e Tutoria, Mostra de Ensino e Extensão. ? Brasília, DF : Embrapa, 2014. p. 161| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | ZMORA, P. E. D. O.; ROMANO, M. R.; WINK, C.; MORALES, M. M.; TONINI, H. CRESCIMENTO DE ESPÉCIES FLORESTAIS INDICADAS PARA USO ENERGÉTICO EM SISTEMA AGROFLORESTAL, SINOP (MT). In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 10, 2016, Cuiabá. SAF: aprendizados, desafios e perspectivas. SBSAF, 2016| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Mandioca e Fruticultura. |
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| 9. | | FAVARO, R. M.; SANTANA, D. L. de Q.; WINK, C.; FARIA, F. D. Primeiro registro de Blastopsylla occidentalis Taylor, 1985 - (Hemiptera: Psyllidae). In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 4., 2005, Colombo. Anais. Colombo: Embrapa Florestas, 2005. 1 CD-ROM (Embrapa Florestas. Documentos, 117). EVINCI. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 12. | | DEON, A.; ZAMORA, P. E. o.; WINK, C.; ROMANO, M. R. Fitomassa de eucalipto e acacia mangium willd. em sistema silvibananeiro no Mato Grosso. In: CONGRESSO FLORESTAL DE MATO GROSSO, 1.; SIMPÓSIO DE PÓS-GRADUAÇÃO EM CIÊNCIAS AMBIENTAIS E FLORESTAIS, 5.; SEMANA ACADÊMICA DE ENGENHARIA FLORESTAL - UFMP/Sinop, 5., 2015, Sinop. Anais. Sinop: UFMT, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | WINK, C.; SANTANA, D. L. de Q.; FARIA, F. D.; ANDRADE, F. M. de; DEDECEK, R. A. Avaliação da mesofauna do solo como determinante no potencial de uso de resíduos de Pinus taeda em plantações florestais. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 4., 2005, Colombo. Anais. Colombo: Embrapa Florestas, 2005. 1 CD-ROM (Embrapa Florestas. Documentos, 117). EVINCI. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 15. | | WINK, C.; LANGE, A.; ARAÚJO, K. Z.; ALMEIDA, A. P. S. de; BEHLING, M.; WRUCK, F. J. Biomassa e nutrientes de eucalipto cultivado em sistema agrossilvipastoril. Nativa, v. 6, n. esp., p. 754-762, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 16. | | CURTO, R. de A.; PINTO, M. F.; ARAÚJO, E. J. G. de; WINK, C.; TONINI, H.; LAURO, A. C. Exatidão de métodos para estimativa do volume de árvores em pé em sistema de integração lavoura-pecuária-floresta. Revista Brasileira de Biometria, v. 37, n. 3, p. 324-334, set. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 17. | | THOMAZ, D. T.; SANTANA, D. L. de Q.; ROSADO-NETO, G. H.; MEDEIROS, A. C. de S.; ABREU, D. C. A. de; WINK, C.; ROVEDO, S. F. Herbivoria de insetos (Coleoptera: Curculionidae) em frutos, sementes e plântulas de guaçatonga (Casearia lasiophylla Eichl), na Região Metropolitana de Curitiba, Paraná, Brasil. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife. Entomologia: da academia à transferência de tecnologia: resumos. Recife: Sociedade Entomológica do Brasil, 2006. CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 18. | | WINK, C.; SANTANA, D. L. de Q.; THOMAZ, D. T.; ANDRADE, F. M. de; SILVA, H. D. da; ANDRADE, G. de C. Diferentes tipos de resíduos de Pinus taeda e grau de decomposição afetando algumas comunidades biológicas em solos florestais. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife. Entomologia: da academia à transferência de tecnologia: resumos. Recife: Sociedade Entomológica do Brasil, 2006. CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 19. | | SILVEIRA, J. G. da; WINK, C.; CESÁRIO, F. V.; ARMACOLO, N. M.; NOGUEIRA, A. K. da S.; SILVA, J. J. N. da; LEITE, F. F. G. D.; CONCEIÇÃO, M. C. G. da; OLIVEIRA NETO, S. N. de; RODRIGUES, R. de A. R. Understanding the flux of nitrous oxide from the eucalypt soil in monoculture and Integrated Crop-Livestock-Forest systems. Australian Journal of Crop Science, v. 16, n. 1, p. 128-136, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 19 | |
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