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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
01/12/2022 |
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Data da última atualização: |
01/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, N. A. de; MARANI, M. M.; LOPES, A. L. F.; SILVA, E. M.; BARBOSA, E. A.; VASCONCELOS, A. G.; KUZNIEWSKI, F. T. B.; LUSTOSA, S. S.; GOMES, K. P.; COLUGNATI, D. B.; ROCHA, J. A.; SANTOS, L. H.; BEMQUERER, M. P.; QUELEMES, P.; VÉRAS, L.; MOREIRA, D. C.; GADELHA, K. K. L.; MAGALHÃES, P. J. C.; PLÁCIDO, A.; EATON, E.; NICOLAU, L.; MEDEIROS, J. V. R.; LEITE, J. R. S. A. |
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Afiliação: |
NAYARA ALVES DE SOUSA, Universidade Federal do Delta do Parnaíba; MARIELA M. MARANI, IPEEC-CONICET; ANDRÉ LUÍS FERNANDES LOPES, Universidade Federal do Delta do Parnaíba; EMANUELLE MORAIS SILVA, Universidade Federal do Delta do Parnaíba; EDER ALVES BARBOSA, Universidade de Brasília; ANDREANNE GOMES VASCONCELOS, Universidade de Brasília; FELIPE T. B. KUZNIEWSKI, Universidade de Brasília; SUELLEN SOUSA LUSTOSA, Universidade Federal do Delta do Parnaíba; KARINA PEREIRA GOMES, Universidade Federal de Goiás; DIEGO BASILE COLUGNATI, Universidade Federal de Goiás; JEFFERSON A. ROCHA, Universidade Federal do Maranhão; LUCIANNA HELENE SANTOS, Universidade Federal de Minas Gerais; MARCELO PORTO BEMQUERER, CNPGL; PATRICK QUELEMES, Universidade Federal do Delta do Parnaíba; LEIZ VÉRAS, Universidade Federal do Delta do Parnaíba; DANIEL C. MOREIRA, Universidade de Brasília; KALINNE KELLY LIMA GADELHA, Universidade Federal do Ceará; PEDRO JORGE CALDAS MAGALHÃES, Universidade Federal do Ceará; ALEXANDRA PLÁCIDO, Universidade do Porto; PETER EATON, Universidade do Porto; LUCAS NICOLAU, Universidade Federal do Delta do Parnaíba; JAND VENES R. MEDEIROS, Universidade Federal do Delta do Parnaíba; JOSÉ R. S. A. LEITE, Universidade de Brasília. |
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Título: |
BR-bombesin: a novel bombesin-related peptide from the skin secretion of the Chaco tree frog (Boana raniceps) with physiological gastric effects. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Amino Acids, v. 54, p. 733-747, 2022. |
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DOI: |
https://doi.org/10.1007/s00726-021-03114-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Bombesin mediates several biological activities in the gastrointestinal (GI) tract and central nervous system in mammals, including smooth muscle contraction, secretion of GI hormones and regulation of homeostatic mechanisms. Here, we report a novel bombesin-like peptide isolated from Boana raniceps. Its amino acid sequence, GGNQWAIGHFM-NH2, was identified and structurally confirmed by HPLC, MS/MS and 454-pyrosequencing; the peptide was named BR-bombesin. The effect of BR-bombesin on smooth muscle contraction was assessed in ileum and esophagus, and its anti-secretory activity was investigated in the stomach. BR-bombesin exerted significant contractile activity with a concentration?response curve similar to that of commercially available bombesin in ileum strips of Wistar rats. In esophageal strips, BR-bombesin acted as an agonist, as many other bombesin-related peptides act, although with different behavior compared to the muscarinic agonist carbachol. Moreover, BR-bombesin inhibited stomach secretion by approximately 50% compared to the untreated control group. This novel peptide has 80% and 70% similarity with the 10-residue C-terminal domain of human neuromedin B (NMB) and human gastrin releasing peptide (GRP10), respectively. Molecular docking analysis revealed that the GRP receptor had a binding energy equal to - 7.3 kcal.mol?1 and ? 8.5 kcal.mol?1 when interacting with bombesin and BR-bombesin, respectively. Taken together, our data open an avenue to investigate BR-bombesin in disorders that involve gastrointestinal tract motility and acid gastric secretion. MenosBombesin mediates several biological activities in the gastrointestinal (GI) tract and central nervous system in mammals, including smooth muscle contraction, secretion of GI hormones and regulation of homeostatic mechanisms. Here, we report a novel bombesin-like peptide isolated from Boana raniceps. Its amino acid sequence, GGNQWAIGHFM-NH2, was identified and structurally confirmed by HPLC, MS/MS and 454-pyrosequencing; the peptide was named BR-bombesin. The effect of BR-bombesin on smooth muscle contraction was assessed in ileum and esophagus, and its anti-secretory activity was investigated in the stomach. BR-bombesin exerted significant contractile activity with a concentration?response curve similar to that of commercially available bombesin in ileum strips of Wistar rats. In esophageal strips, BR-bombesin acted as an agonist, as many other bombesin-related peptides act, although with different behavior compared to the muscarinic agonist carbachol. Moreover, BR-bombesin inhibited stomach secretion by approximately 50% compared to the untreated control group. This novel peptide has 80% and 70% similarity with the 10-residue C-terminal domain of human neuromedin B (NMB) and human gastrin releasing peptide (GRP10), respectively. Molecular docking analysis revealed that the GRP receptor had a binding energy equal to - 7.3 kcal.mol?1 and ? 8.5 kcal.mol?1 when interacting with bombesin and BR-bombesin, respectively. Taken together, our data open an avenue to investigate BR-bo... Mostrar Tudo |
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Palavras-Chave: |
Peptídeo bioativo; Secreção gástrica. |
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Thesagro: |
Anfíbio; Peptídeo. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02899naa a2200445 a 4500
001 2149045
005 2022-12-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00726-021-03114-4$2DOI
100 1 $aSOUSA, N. A. de
245 $aBR-bombesin$ba novel bombesin-related peptide from the skin secretion of the Chaco tree frog (Boana raniceps) with physiological gastric effects.$h[electronic resource]
260 $c2022
520 $aBombesin mediates several biological activities in the gastrointestinal (GI) tract and central nervous system in mammals, including smooth muscle contraction, secretion of GI hormones and regulation of homeostatic mechanisms. Here, we report a novel bombesin-like peptide isolated from Boana raniceps. Its amino acid sequence, GGNQWAIGHFM-NH2, was identified and structurally confirmed by HPLC, MS/MS and 454-pyrosequencing; the peptide was named BR-bombesin. The effect of BR-bombesin on smooth muscle contraction was assessed in ileum and esophagus, and its anti-secretory activity was investigated in the stomach. BR-bombesin exerted significant contractile activity with a concentration?response curve similar to that of commercially available bombesin in ileum strips of Wistar rats. In esophageal strips, BR-bombesin acted as an agonist, as many other bombesin-related peptides act, although with different behavior compared to the muscarinic agonist carbachol. Moreover, BR-bombesin inhibited stomach secretion by approximately 50% compared to the untreated control group. This novel peptide has 80% and 70% similarity with the 10-residue C-terminal domain of human neuromedin B (NMB) and human gastrin releasing peptide (GRP10), respectively. Molecular docking analysis revealed that the GRP receptor had a binding energy equal to - 7.3 kcal.mol?1 and ? 8.5 kcal.mol?1 when interacting with bombesin and BR-bombesin, respectively. Taken together, our data open an avenue to investigate BR-bombesin in disorders that involve gastrointestinal tract motility and acid gastric secretion.
650 $aAnfíbio
650 $aPeptídeo
653 $aPeptídeo bioativo
653 $aSecreção gástrica
700 1 $aMARANI, M. M.
700 1 $aLOPES, A. L. F.
700 1 $aSILVA, E. M.
700 1 $aBARBOSA, E. A.
700 1 $aVASCONCELOS, A. G.
700 1 $aKUZNIEWSKI, F. T. B.
700 1 $aLUSTOSA, S. S.
700 1 $aGOMES, K. P.
700 1 $aCOLUGNATI, D. B.
700 1 $aROCHA, J. A.
700 1 $aSANTOS, L. H.
700 1 $aBEMQUERER, M. P.
700 1 $aQUELEMES, P.
700 1 $aVÉRAS, L.
700 1 $aMOREIRA, D. C.
700 1 $aGADELHA, K. K. L.
700 1 $aMAGALHÃES, P. J. C.
700 1 $aPLÁCIDO, A.
700 1 $aEATON, E.
700 1 $aNICOLAU, L.
700 1 $aMEDEIROS, J. V. R.
700 1 $aLEITE, J. R. S. A.
773 $tAmino Acids$gv. 54, p. 733-747, 2022.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 13 | |
| 1. |  | MARTINATI, J. C.; CARDOSO, D. C.; GUERREIRO-FILHO, O.; VIDAL, R. O.; CARAZZOLLE, M. F.; MALUF, M. P. Caracterização molecular da interação cafeeiro/bicho-mineiro. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 2. | | MARTINATI, J. C.; CARDOSO, D. C.; VIDAL, R. O.; CARAZZOLLE, M. F.; GIACHETTO, P. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. Large-escale expression of genes related to phytoalexins, phenols, flavonoids and lignin biosynthesis in coffee plants infested with leaf-miner. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 83. B11. ASIC 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
|  |
| 3. | | CARDOSO, D. C.; MARTINATI, J. C.; GIACHETTO, P. F.; VIDAL, R. O.; CARAZZOLLE, M. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P. Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection. BMC Genomics, London, v. 15, p. 1-20, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café. |
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| 4. | | MARTINATI, J. C.; CARDOSO, D. C.; VIDAL, R. O.; CARAZZOLLE, M. F.; GIACHETTO, P. F.; PADILHA, L.; OLIVEIRA FILHO, G.; MALUF, M. P. Large-scale expression of genes related to phytoalexins, phenols, flavonoids and lignin biosynthesis in coffee plants infested with leaf-miner. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 83 B11. ASIC 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 5. | | HERAI, R. H.; VIDAL, R. O.; CARAZZOLLE, M. F.; COSTA, G. G. L.; FALCAO, P. R. K.; YAMAGISHI, M. E. B.; FRANCHINI, K. G.; PEREIRA, G. G. A. CNBi: the new Brazilian National Consortium for Bioinformatics. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010. p. 110. X-meeting 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 6. | | VIDAL, R. O.; ALEKCEVETCH, J. C.; LEROY, T.; DE BELLIS, F.; POT, D.; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. High-through put sequencing of CDNA shows that CV. Rubi and IAPAR59 of Coffea Arabica have different molecular response to water privation. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | HERAI, R. H.; COSTA, G. G. D. L.; R. JÚNIOR, O.; VIDAL, R. O.; NASCIMENTO, L. C.; PARIZZI, L. P.; PEREIRA, G. G. A.; CARAZZOLLE, M. F. TORNADO: an automated pipeline for de novo hybrid genome assembly based on free software packages for sanger and next generation sequencing technologies (NGS). In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010. p. 119. X-meeting 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 8. | | NASCIMENTO, L. C.; COSTA, G. G. L.; VIDAL, R. O.; MEYER, L.; BINNECK, E.; KIDO, E. A.; RODRIGUES, F.; KULCHESKI, F. R.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; PEREIRA, G. A. G.; CARAZZOLLE, M. F. Bioinformatics analysis applied to genosoja project. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 5., 2009, Angra dos Reis. Abstracts book ... Rio de Janeiro: AB3C, 2009. p. 22. X-Meeting 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | MONDEGO, J. M. C.; VIDAL, R. O.; CARAZZOLLE, M. F.; TOKUDA, E. K.; PARIZZI, L. P.; COSTA, G. G. L.; PEREIRA, L. F. P.; ANDRADE, A. C.; COLOMBO, C. A.; VIEIRA, L. G. E.; PEREIRA, G. A. G. An EST-based analysis identifies new genes and reveals distinctive gene expression features of Coffea arabica and Coffea canephora. BMC Plant Biology, v.11, n. 30, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | NASCIMENTO, L. C.; VIDAL R. O.; MONDEGO, J. M. C.; COSTA, G. G. L.; JUNIOR, O. R.; RODRIGUES, F.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; PEREIRA, G. A. G.; CARAZZOLLE, M. F. Genotipagem de cultivares brasileiros de soja através da detecção de SNPs. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | VIDAL, R. O.; MONDEGO, J. M. C.; POT, D.; AMBRÓSIO, A. B.; ANDRADE, A. C.; PEREIRA, L. F. P.; COLOMBO, C. A.; VIEIRA, L. G. E.; CARAZZOLLE, M. F.; PEREIRA, G. A. G. A high-throughput data mining of single nucleotide polymorphisms in Coffea species expressed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica. PLANT PHYSIOLOGY, v. 154, p. 1053-1066. 2010. 1053-1066| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. BMC Plant Biology, v. 16, n. 94, p. 1-18, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café. |
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| 13. | | MONDEGO, J. M. C.; CARAZZOLLE, M. F.; COSTA, G. G. L.; FORMIGHIERI, E. F.; PARIZZI, L. P.; RINCONES, J.; COTOMACCI, C.; CARRARO, D. M.; CUNHA, A. F.; CARRER, H.; VIDAL, R. O.; ESTRELA, R. C.; GARCÍA, O.; THOMAZELLA, D. P. T.; OLIVEIRA, B. V.; PIRES, A. B. L.; RIO, M. C. S.; ARAÚJO, M. R. R.; CASTRO, A. L. B.; GRAMACHO, K. P.; GONÇALVES, M. S.; GÓES NETO, A.; BARBOSA, L. V.; GUILTINAM, M.; BAILEY, B.; MEINHARDT, L. W.; CASCARDO, J. C. M.; PEREIRA, G. A. G. The genome survey of Moniliophthora perniciosa, the causal agent of cacao witches' broom disease, gives insights about novel phytopathogenicity mechanisms. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 13 | |
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