| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
12/05/2020 |
|
Data da última atualização: |
02/06/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BRITO, F. S. D.; SANTOS, J. R. P.; AZEVEDO, V. C. R.; PEIXOUTO, Y. S.; OLIVEIRA, S. A. S. de; FERREIRA, C. F.; HADDAD, F.; AMORIM, E. P.; FRAAIJE, B.; MILLER, R. N. G. |
|
Afiliação: |
FABIANE S. D. BRITO; JANSEN R. P. SANTOS; VÂNIA C. R. AZEVEDO; YSLAI S. PEIXOUTO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDO HADDAD, CNPMF; EDSON PERITO AMORIM, CNPMF; BART FRAAIJE; ROBERT N. G. MILLER. |
|
Título: |
Genetic Diversity and Azole fungicide sensitivity in pseudocercospora musae field populations in Brazil. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Frontiers in Microbiology, v, 11, n.99, 2020. |
|
ISSN: |
1664-302X |
|
DOI: |
10.3389/fmicb.2020.00099 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Pseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel?s test (r = 0.19, P < 0.001). On the basis of DMI fungicide sensitivity testing and CYP51 gene sequence polymorphism, isolates from the Distrito Federal separated into two main groups, one with generally higher EC50 values against eight DMI fungicides. A clear phenotype-to-genotype relationship was observed for isolates carrying the CYP51 alteration Y461N. Conventionally adopted fungicides for control of Sigatoka leaf spot are likely to be overcome by combined sexual and asexual reproduction mechanisms in P. musae driving genetic variability. Continued analysis of pathogen genetic diversity and monitoring of DMI sensitivity profiles of Brazilian field populations is essential for the development of integrated control strategies based on host resistance breeding and rational design of fungicide regimes. MenosPseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel?s test ... Mostrar Tudo |
|
Thesagro: |
Banana; Sigatoka Negra. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03118naa a2200277 a 4500
001 2122242
005 2020-06-02
008 2020 bl uuuu u00u1 u #d
022 $a1664-302X
024 7 $a10.3389/fmicb.2020.00099$2DOI
100 1 $aBRITO, F. S. D.
245 $aGenetic Diversity and Azole fungicide sensitivity in pseudocercospora musae field populations in Brazil.$h[electronic resource]
260 $c2020
520 $aPseudocercospora musae, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana (Musa spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte. Resistance to demethylation inhibitor (DMI) fungicides was also examined. For 162 isolates from 10 banana growing regions, analysis of mating type idiomorph frequency was conducted, together with estimation of genetic diversity at 15 microsatellite loci. A total of 149 haplotypes were identified across the examined populations, with an average genetic diversity of 4.06. In general, populations displayed 1:1 proportions of idiomorphs MAT1-1 and MAT1-2, providing evidence for sexual recombination. Multilocus linkage disequilibrium also indicated asexual reproduction contributing to the genetic structure of certain populations. AMOVA revealed that 86.3% of the genetic differentiation of the pathogen occurred among isolates within populations. Discriminant Analysis of Principal Components (DAPC) identified six most probable genetic groups, with no population structure associated with geographic origin or collection site. Although genetic similarity was observed among certain populations from different states, data revealed increasing genetic differentiation with increasing geographic distance, as validated by Mantel?s test (r = 0.19, P < 0.001). On the basis of DMI fungicide sensitivity testing and CYP51 gene sequence polymorphism, isolates from the Distrito Federal separated into two main groups, one with generally higher EC50 values against eight DMI fungicides. A clear phenotype-to-genotype relationship was observed for isolates carrying the CYP51 alteration Y461N. Conventionally adopted fungicides for control of Sigatoka leaf spot are likely to be overcome by combined sexual and asexual reproduction mechanisms in P. musae driving genetic variability. Continued analysis of pathogen genetic diversity and monitoring of DMI sensitivity profiles of Brazilian field populations is essential for the development of integrated control strategies based on host resistance breeding and rational design of fungicide regimes.
650 $aBanana
650 $aSigatoka Negra
700 1 $aSANTOS, J. R. P.
700 1 $aAZEVEDO, V. C. R.
700 1 $aPEIXOUTO, Y. S.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aFERREIRA, C. F.
700 1 $aHADDAD, F.
700 1 $aAMORIM, E. P.
700 1 $aFRAAIJE, B.
700 1 $aMILLER, R. N. G.
773 $tFrontiers in Microbiology, v, 11$gn.99, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 7 | |
| 1. |  | EIRA, M. T. S.; FAIAD, M. G. R.; FREIRE, M. S.; VASCONCELOS, L. C.; BARBOSA, N. F. Controle de qualidade do feijao (Phaseolus vulgaris) conservado no Banco de Germoplasma do CENARGEN-EMBRAPA. In: SEMINARIO PANAMERICANO DE SEMILLAS, 15., WORKSHOP SOBRE MARKETING EM SEMENTES E MUDAS, 3., 1996, Gramado, RS. Anais. [S.l.]: Federation Lationamericana de Asociaciones de Semillas, 1996. p.75 p.75| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|   |
| 2. | | CASTRO, K. N. de C.; LIMA, D. F.; VASCONCELOS, L. C.; MAGALHAES, J. A.; COSTA, J. V.; SANTOS, R. C. dos. Ação de óleos e extratos vegetais no controle de Rhipicephalus (Boophilus) microplus in vitro. Cadernos de Agroecologia, Cruz Alta, RS, v. 6, n. 2, dez. 2011. 6 p. Edição dos Resumos do 7º Congresso Brasileiro de Agroecologia, Fortaleza-CE, dez. 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
|  |
| 3. | | CASTRO, K. N. de C.; LIMA, D. F.; VASCONCELOS, L. C.; LEITE, J. R. S. A.; SANTOS, R. C.; PAZ NETO, A. A.; COSTA-JÚNIOR, L. M. Acaricide activity in vitro of Acmella oleracea against Rhipicephalus microplus. Parasitology Research, v. 113, n. 10, p. 3697-3701, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 5. | | CASTRO, K. N. de C.; VASCONCELOS, L. C.; COSTA, J. V.; SANTOS, R. C. dos; ANDRADE, I. M. de; RIBEIRO, V. Q. Extratos vegetais no controle in vitro do carrapato dos bovinos. Cadernos de Agroecologia, Cruz Alta, RS, v. 6, n. 2, dez. 2011. 5 p. Edição dos Resumos do 7º Congresso Brasileiro de Agroecologia, Fortaleza-CE, dez. 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 6. | | BARRO, D. M. A.; ANDRADE, I. M. de; SILVA FILHO, F. A. e; COSTA, J. V.; VASCONCELOS, L. C.; MAGALHAES, J. A.; CASTRO, K. N. de C. Eficácia in vitro do óleo essencial de Lantana camara sobre Rhipicephalus (Boophilus) microplus. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 10., 2015, Teresina. [Anais...]. Teresina: Sociedade Nordestina de Produção Animal, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 7. | | CASTRO, K. N. de C.; LIMA, D. F.; VASCONCELOS, L. C.; SANTOS, R. C.; PEREIRA, A. M. L.; FOGACA, F. H. dos S.; CANUTO, K. M.; BRITO, E. S. de; CALVET, R. M. Composição química e eficácia do óleo essencial e do extrato etanólico de Alpinia zerumbet sobre Staphylococcus aureus. Arquivos do Instituto Biológico, São Paulo, v. 83, p. 1-7, e0192014, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Meio-Norte. |
| |
| Registros recuperados : 7 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
