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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/05/2009 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. |
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Afiliação: |
NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA/USP; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA/USP; TATIANA ALMEIDA DRUMOND TETZNER, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009. |
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DOI: |
10.1089/clo.2008.0044 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. MenosThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst ... Mostrar Tudo |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02377naa a2200205 a 4500
001 1048984
005 2023-03-15
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1089/clo.2008.0044$2DOI
100 1 $aSARAIVA, N. Z.
245 $aDemecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes.$h[electronic resource]
260 $c2009
520 $aThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine.
650 $aoocytes
700 1 $aPERECIN, F.
700 1 $aMÉO, S. C.
700 1 $aFERREIRA, C. R.
700 1 $aTETZNER, T. A. D.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells$gv. 11, n. 1, p. 141-152, mar. 2009.
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| Registros recuperados : 34 | |
| 8. |  | MOURA, J. I. L.; CIVIDANES, F. J.; SANTOS FILHO, L. P. dos; VALLE, R. R. Polinização do dendezeiro por besouros no Sul da Bahia. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 3, p. 289-294, mar. 2008 Título em inglês: Pollination of oil palm by weevils in Southern Bahia, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | VALLE, R. V. do; ELOY, A. M. X.; SILVA, N. M. M. da; FURTADO, J. R.; MEDEIROS, J. N. de. Caracterização de proteínas do plasma seminal de caprinos da raça Moxotó no semiárido Nordestino. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. 3 f. 1 CD-ROM. Título em inglês: Instructions for elaborating abstract of Annual Meeting of Animal Science Brazilian Association.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | SILVA, N. M. M.; VALLE, R. V. do; ELOY, A. M. X.; FACO, O.; PINHEIRO, R. R.; FURTADO, J. R. Influência do fator animal e época do ano nas características espermáticas e proteínas totais em ovinos da raça Morada Nova no Semiárido Nordestino. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. 3 f. 1 CD-ROM. Título em inglês: Instructions for elaborating abstract of Annual Meeting of Animal Science Brazilian Association.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | SANTOS, F. C. P. dos; VALLE, R. V. do; SILVA, N. M. M.; BARBOSA, M. de M.; PERES, M. C. R.; ELOY, A. M. X.; FURTADO, J. R. Caracterização dos perfis eletroforéticos de proteínas do plasma seminal e sanguíneo de caprinos da raça Moxotó. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 7., 2010, Sobral. Iniciação para o semiárido: [Resumos]. Sobral: Universidade Estadual Vale do Acaraú, 2010. 1 CD ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | SILVA, N. M. M.; ELOY, A. M. X.; FURTADO, J. R.; VALLE, R. do V.; SOUZA, A. Z. B. de; SILVA, N. M. Avaliações clínicas em ovinos Morada Nova e sua relação com a época do ano na região Nordeste. In: ZOOTEC NA AMAZÔNIA LEGAL, 1.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 20., 2010, Palmas. Sustentabilidade e produção animal. Araguaiana: Universidade Federal de Tocantins: Associação Brasileira de Zootecnistas, 2010. 3 f. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | RAINER, K. T.; COLOMBI, B. L.; GONÇALVES, M. J.; HELM, C. V.; TAVARES, L. B. B.; VALLE, R. de C. S. C.; VALLE, J. A. B. Laccase production of white rot fungus grown on SBS paperboard coated with PET, aiming the bioadsortion strategy. In: INTERNATIONAL CONFERENCE ON FIBER AND POLYMER BIOTECHNOLOGY, 10., 2018, Balneário Camboriú. Proceedings. Blumenau: FURB, 2018. p. 88.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 18. | | VILLELA, L. C. V.; SILVA, E. C. da; CAETANO, A. R.; ELOY, A. M. X.; VALLE, R. V. do; PAIVA, S. R. Seleção de caprinos Moxotó para Núcleo de Conservação a partir de marcadores microssatélites. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília, DF. Anais... Brasília, DF: SBZ, 2012. 3 f. 1 CD-ROM. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | LIMA, B. M.; TEIXEIRA, J. E. C.; GAZAFFI, R.; GARCIA, A. A. F.; GRATTAPAGLIA, D.; VALLE, R. K. D.; CAMARGO, L. E. A. Identification of a novel QTL contributing to rust resistance in Eucalyptus. BMC Proceedings 2011, v. 5, Suppl 7, p. P32, 2011. Edição dos resumos do IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration, 2011 Arraial d?Ajuda, Bahia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | PEDRI, Z. C.; TAVARES, L. B. B.; LOZANO, L. M. dos S.; HERMANN, K. L.; VALLE, R. de C. S. C.; HELM, C. V.; MAGALHAES, W. L. E. Efeito de fatores de processo na atividade holocelulolítica e no crescimento micelial de Lentinula edodes. In: MOSTRA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 7., 2013, Blumenau. Anais. Blumenau: FURB, 2013. Resumo. Disponível online. MIPE.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 34 | |
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