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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/05/2000 |
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Data da última atualização: |
03/08/2023 |
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Autoria: |
TACHEDJIAN, M.; KRYWULT, J.; MOORE, R. J.; HODGON, A. L. M. |
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Título: |
Caseous lymphadenitis vaccine development: site-specific inactivation of the Corynebacterium pseudotuberculosis phospholipase D gene. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Vaccine, v. 13, n. 18, p. 1785-1792, Dec. 1995. |
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DOI: |
10.1016/0264-410x(95)00144-p. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Vaccines for ovine caseous lymphadenitis (CLA) are currently formulated using partially purified, formalin inactivated phospholipase D (PLD) derived from Corynebacterium pseudotuberculosis culture supernatants. Chemical treatment has been a common and effective way of inactivating bacterial toxins for use in toxoid vaccines. Genetic inactivation of toxin genes using site-specific mutagenesis has the potential to improve this process by providing a safer and more cost-effective product. In the present study amino acid substitutions at the putative catalytic site and metal binding domain of the PLD protein had a profound affect upon PLD activity and secretion from C. pseudotuberculosis. Two mutated PLD analogues that were secreted to a level of 40% compared to the wild-type and retained minimal activity showed promise for development as recombinant CLA vaccines. Further work will be required to establish their suitability for commercialization. |
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Palavras-Chave: |
Base Sequence; Expressão genética; Fosfolipase; Genetic toxoid; Inativacao gene; Molecular Sequence Data. |
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Thesagro: |
Linfadenite Caseosa; Toxina; Vacina. |
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Thesaurus Nal: |
Bacterial vaccines; Caseous lymphadenitis; Formaldehyde; Gene expression; Genetic resistance; Immunology; Phospholipase D; Sheep; Toxins. |
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Categoria do assunto: |
-- |
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Marc: |
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | TRAVASSO, M. I.; MAGRIN, G. O.; BAETHGEN, W. E.; CASTAÑO, J. P.; RODRIGUEZ, G. R.; PIRES, J. L. F.; GIMENEZ, A.; CUNHA, G. R. da; FERNANDES, J. M. C. Maize and soybean cultivation in Southeastern South America: adapting to climate change. In: LEARY, N.; ADEJUWON, J.; BARROS, V.; BURTON, I.; KULKARNI, J.; LASCO, R. Climate change and adaptation. London: Earthscan, 2008. p. 332-352.| Tipo: Capítulo em Livro Técnico-Científico |
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