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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
04/09/2012 |
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Data da última atualização: |
19/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, D. F.; BATISTA, J. S. da S.; SCHIAVON, A. L.; ANDRADE, D. S.; HUNGRIA, M. |
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Afiliação: |
DOUGLAS FABIANO GOMES, UEL; JESIANE STEFÂNIA DA SILVA BATISTA, UNIFIL; ALINE LUIZA SCHIAVON1, UNIFIL; DIVA SOUZA ANDRADE, IAPAR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
BMC Microbiology, London, v. 12, article 84, May 2012. |
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Páginas: |
12 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species. MenosRhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02241naa a2200205 a 4500
001 1932908
005 2024-07-19
008 2012 bl uuuu u00u1 u #d
100 1 $aGOMES, D. F.
245 $aProteomic profiling of Rhizobium tropici PRF 81$bidentification of conserved and specific responses to heat stress.$h[electronic resource]
260 $c2012
300 $a12 p.
520 $aRhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aBATISTA, J. S. da S.
700 1 $aSCHIAVON, A. L.
700 1 $aANDRADE, D. S.
700 1 $aHUNGRIA, M.
773 $tBMC Microbiology, London$gv. 12, article 84, May 2012.
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| 5. |  | WOOD, C. H.; WALKER, R.; PERZ, S.; SILVA, L. G. T.; TONI, F.; CALDAS, M. Segurança na propriedade da terra e a conservação de florestas tropicais na Amazônia Oriental: o caso da fronteira agrícola de Uruará, PA. Belém, PA: Embrapa Amazônia Oriental, 1999. 3 p. (Embrapa Amazônia Oriental. Comunicado técnico, 16).| Tipo: Comunicado Técnico/Recomendações Técnicas |
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| 7. | | COUDEL, E.; FERREIRA, J. N.; AMAZONAS, M. de C.; ELOY, L.; HERCOWITZ, M.; MATTOS, L. M. de; MAY, P.; MURADIAN, R.; PIKETTY, M.-G.; TONI, F. The rise of PES in Brazil: from pilot projects to public policies. In: MARTINEZ-ALIER, J.; MURADIAN, R. (Ed.). Handbook of ecological economics. Cheltenham, UK: Northampton, MA, USA: Edward Elgar Publishing, 2015. p. 450-472.| Tipo: Capítulo em Livro Técnico-Científico |
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| 8. | | COUDEL, E.; AMAZONAS, M.; FERREIRA, J.; ELOY, L.; HERCOWITZ, M.; MATTOS, L. M. de; MAY, P.; MURADIAN, R.; PIKETTY, M. G.; TONI, F. La emergencia de una política de pagos por servicios ambientales en Brasil: de agendas distintas a convergentes? In: DE BLASS, D. E.; LE COQ, J. F.; GUEVARA SANGINES, A. (Coord.). Los pagos por servicios ambientales en América Latina: governanza, impactos y perspectivas. México: Universidade Iberoamericana Ciudad de México, 2017. p. 131-164.| Tipo: Capítulo em Livro Técnico-Científico |
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| 9. | | CALMON, M.; MESQUITA, R. de C. G.; CRESTANA, S.; PINTO, E. de P. P.; CIASCA, B. S.; DRUCKER, D. P.; STABILE, M. de C. C.; TONI, F.; PINTO, L. F. G.; ALTMANN, A.; MAZZETTI, C. Governança para conciliar a agricultura, a biodiversidade e os serviços ecossistêmicos no Brasil. In: PRADO, R. B.; OVERBECK, G. E.; GRACO-ROZA, C.; MOREIRA, R. A.; MONTEIRO, M. M.; DUARTE, G. T. (org.). Relatório temático sobre agricultura, biodiversidade e serviços ecossistêmicos. Campinas: Ed. dos Autores, 2024. cap. 6, p. 160-184.| Tipo: Capítulo em Livro Técnico-Científico |
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| 10. | | ZIMMER, L. E.; KLEIN, C. H.; KUNZ, A.; BIESUS, L. L.; VIZZOTTO, R.; SCHUCK, C. M.; SILVA, M. L. B. da; NICOLOSO, R. da S.; TONI, F. L. de; SCHWINGEL, M. E.; JACOB, J. de S.; MULINARI, M. R.; SUZIN, L. Comitê Local de Gestão Ambiental - CLGA. In: WORKSHOP DO PROJETO ISOEMBRAPA, 2012, Jaguariúna. Modelos de excelência e compartilhamento de boas práticas de gestão: anais... Jaguariúna: Embrapa Meio Ambiente, 2012. 1 CD ROM. Projeto/Plano de Ação: 11.11.11.111.| Tipo: Resumo em Anais de Congresso |
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