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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/05/2025 |
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Data da última atualização: |
27/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. D. R.; SILVA, I. M. da; RAMOS, L. F. C.; NOGUEIRA, F. C. S.; CONTE JUNIOR, C. A.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; BARBOSA, N. O.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, UNIVERSIDADE FEDERAL FLUMINENSE; YARA MARTINS DA SILVA, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUÍS FELIPE COSTA RAMOS, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; FÁBIO CÉSAR SOUSA NOGUEIRA, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; CARLOS ADAM CONTE JUNIOR, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; NATHÁLIA OLIVEIRA BARBOSA, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 192, 1057062025, Aug. 2025. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2025.105706 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Hormonal protocols for cervical dilation (HPCD) are essential for non-surgical embryo recovery in ewes, due to the cervical complexity of this species. However, the hormones used for this purpose can alter the uterine protein profile and impact the embryonic quality. To test this hypothesis, Santa Inês ewes were superovulated and subjected or not to the HPCD before uterine lavage and embryos recovery by laparotomy. The protein profile of the uterine lavage was determined by mass spectrometry. Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 17 proteins were highly expressed in the treated group (P < 0.05), such as glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundant in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection. Highlights: The protein profile of the uterine fluid of ewes was determined. Proteins most abundantly are related to prostaglandin biosynthesis. Proteins most abundantly are related to cellular and metabolic processes. The hormonal protocol for cervical dilation modifies the uterine protein profile. MenosAbstract: Hormonal protocols for cervical dilation (HPCD) are essential for non-surgical embryo recovery in ewes, due to the cervical complexity of this species. However, the hormones used for this purpose can alter the uterine protein profile and impact the embryonic quality. To test this hypothesis, Santa Inês ewes were superovulated and subjected or not to the HPCD before uterine lavage and embryos recovery by laparotomy. The protein profile of the uterine lavage was determined by mass spectrometry. Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 17 proteins were highly expressed in the treated group (P < 0.05), such as glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundant in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection. Highlights: The protein profile of the uterine fluid of ewes was determined. Proteins most abundantly are related to prostaglandin biosynthesi... Mostrar Tudo |
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Palavras-Chave: |
Embryo collection; MOET; Protocolo hormonal; Tratamentos hormonais. |
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Thesagro: |
Biossíntese; Ovelha; Ovino; Prostaglandina; Reprodução; Superovulação. |
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Thesaurus Nal: |
Biosynthesis; Prostaglandins; Proteomics; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02902naa a2200421 a 4500
001 2176044
005 2025-05-27
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2025.105706$2DOI
100 1 $aSANTOS, J. D. R.
245 $aHormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes.$h[electronic resource]
260 $c2025
520 $aAbstract: Hormonal protocols for cervical dilation (HPCD) are essential for non-surgical embryo recovery in ewes, due to the cervical complexity of this species. However, the hormones used for this purpose can alter the uterine protein profile and impact the embryonic quality. To test this hypothesis, Santa Inês ewes were superovulated and subjected or not to the HPCD before uterine lavage and embryos recovery by laparotomy. The protein profile of the uterine lavage was determined by mass spectrometry. Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 17 proteins were highly expressed in the treated group (P < 0.05), such as glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundant in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection. Highlights: The protein profile of the uterine fluid of ewes was determined. Proteins most abundantly are related to prostaglandin biosynthesis. Proteins most abundantly are related to cellular and metabolic processes. The hormonal protocol for cervical dilation modifies the uterine protein profile.
650 $aBiosynthesis
650 $aProstaglandins
650 $aProteomics
650 $aReproduction
650 $aSheep
650 $aBiossíntese
650 $aOvelha
650 $aOvino
650 $aProstaglandina
650 $aReprodução
650 $aSuperovulação
653 $aEmbryo collection
653 $aMOET
653 $aProtocolo hormonal
653 $aTratamentos hormonais
700 1 $aSILVA, I. M. da
700 1 $aRAMOS, L. F. C.
700 1 $aNOGUEIRA, F. C. S.
700 1 $aCONTE JUNIOR, C. A.
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aBARBOSA, N. O.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tResearch in Veterinary Science$gv. 192, 1057062025, Aug. 2025.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 8 | |
| 2. |  | CÓRDOVA, H. de A.; ALESSIO, D. R. M.; CARDOZO, L. L.; THALER NETO, A. Impact of the factors of animal production and welfare on robotic milking frequency. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 2, p. 238-246, Fev. 2018. Título em português: Impacto dos fatores de produção e bem-estar animal sobre a frequência de ordenha robotizada.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | GABBI, A. M.; MCMANUS, C. M.; ZANELA, M. B.; STUMPF, M. T.; BARBOSA, R. S.; FRUSCALSO, V.; THALER NETO, A.; SCHMIDT, F. A.; FISCHER, V. Milk traits of lactating cows submitted to feed restriction. Tropical Animal Health and Production, v. 48, n. 1, p. 37-43, Jan. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Semiárido. |
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| 4. | | RECHE, N. L. M.; CARDOZO, L. L.; THALER NETO, A.; SOUZA, G. N. de; WERNCKE, D.; SIMON, E. E.; FELIPUS, N. C.; PEREIRA, I. B. Fatores de risco associados a novas infecções intramamárias em rebanhos leiteiros do Estado de Santa Catarina. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | RECHE, N. L. M.; CARDOZO, L. L.; THALER NETO, A.; SOUZA, G. N. de; WERNCKE, D.; SCHMIDT, F. A.; FELIPUS, N. C.; SIMON, E. E. Fatores de risco para mastite subclínica crônica em rebanhos leiteiros do Estado de Santa Catarina. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 3 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | CARDOZO, L. L.; THALER NETO, A.; SOUZA, G. N. de; WERNCKE, D.; MACHADO, N. L.; SCHMIDT, F. A.; FELIPUS, N. C.; SIMON, E. E. Fatores associados a hiperqueratose na extremidade dos tetos em bovinos leiteiros no estado de Santa Catarina. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 5., 2013, Botucatu. Anais. Curitiba: Conselho Brasileiro de Qualidade do Leite, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | CARDOZO, L. L.; THALER NETO, A.; SOUZA, G. N. de; WERNCKE, D.; SCHMIDT, F. A.; MACHADO, N. L.; FELIPUS, N. C.; PEREIRA, I. B. Relação entre hiperqueratose na extremidade dos tetos e o escore de células somáticas. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 5., 2013, Botucatu. Anais. Curitiba: Conselho Brasileiro de Qualidade do Leite, 2013. p. 322-323| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | CARDOZO, L. L.; THALER NETO, A.; SOUZA, G. N. de; PICININ, L. C. A.; FELIPUS, N. C.; RECHE, N. L. M.; SCHMIDT, F. A.; WERNCKE, D.; SIMON, E. E. Risk factors for the occurrence of new and chronic cases of subclinical mastitis in dairy herds in southern Brazil. Journal of Dairy Science, v. 98, n. 11, p. 7675-7685, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 8 | |
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