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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, E. T. de A.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. |
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Afiliação: |
ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ; CARLOS HENRIQUE SALVINO GADELHA MENESES, UEPB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Annals of Applied Biology, Annals of Applied Biology, v. 180, p. 163-175. 2022. |
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DOI: |
DOI: 10.1111/aab.12721 |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. MenosGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, th... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial peptide; Bactericidal effect. |
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Thesaurus Nal: |
Biological control; Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 9 | |
| 2. |  | ASSINE, M. L.; MACEDO, H. A.; STEVAUX, J. C.; BERGIER, I.; PADOVANI, C. R.; SILVA, A. Avulsive rivers in the hydrology of the Pantanal wetland. In: BERGIER, I.; ASSINE, M. L. (Ed.). Dynamics of the Pantanal Wetland in South America. Switzerland: Springer International Publishing, 2016. p. 83-110. (The Handbook of Environmental Chemistry, 37)| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | RASBOLD, G. G.; McGLUE, M. M.; STEVAUX, J. C.; PAROLIN, M.; SILVA, A.; BERGIER, I. Sponge spicule and phytolith evidence for Late Quaternary environmental changes in the tropical Pantanal wetlands of western Brazil. Palaeogeography, Palaeoclimatology, Palaeoecology, v. 518, p. 119-133, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 7. | | MACEDO, H. de A.; STEVAUX, J. C.; SILVA, A.; MERINO, E. R.; PUPIM, F. do N.; BERGIER, I. Methodology for analysis of distribution and classification of the intensity of dequada fish kill events in the Pantanal wewtland. GEOGRAFIA, Rio Claro, v. 40, Número Especial, p. 163-176, ago. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | RASBOLD, G. G.; MCGLUE, M. M.; STEVAUX, J. C.; PAROLIN, M.; SILVA, A.; BERGIER, I.; LUZ, L. D. Microfossil and stratigraphic evidence for late quaternary paleoenvironmental changes at Lagoa Negra, Central Pantanal wetlands (Brazil). In: FALL MEETING, 2018, Washington, D.C. Abstracts... Washington, D.C: American Geophysical Union, 2018. Não paginado. AGU 2018. Session PP23D-1525.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 9. | | MACEDO, H. de A.; STEVAUX, J. C.; SILVA, A.; MERINO, E. R.; PUPIM, F. do N.; BERGIER, I. Distribuição e classificação da intensidade do fenômeno de dequada no Pantanal. In: SIMPÓSIO DE GEOTECNOLOGIAS NO PANTANAL, 5., 2014, Campo Grande, MS. Anais... São José dos Campos: INPE, 2014. p. 720-730| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 9 | |
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