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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/03/1996 |
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Data da última atualização: |
23/05/2023 |
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Autoria: |
LI, R.; CAMERON, W. N.; BATT, P. A.; TROUNSON, O. A. |
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Título: |
Maximum survival of frozen goat embryos is attained at the expanded, hatching and hatched blastocyst stages of development. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 2, n. 4, p. 345-350, 1990. |
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DOI: |
10.1071/rd9900345. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Embryos were collected 5, 6 and 7 days after ovulation to determine their optimum stage of development for cryopreservation. The embryos were frozen using either glycerol or dimethyl sulfoxide (DMSO) as cryoprotectant, and then thawed and cultured to determine their viability. The rate of survival increased as the stage of development of the embryos increased. All hatched blastocysts survived freezing regardless of which cryoprotectant was used, whereas none of the 22 morulae frozen with DMSO survived, and only 12 of the 63 morulae frozen with glycerol survived. At the early blastocyst stage there was a significantly different result between freezing procedures (P less than 0.05) with greater survival rates obtained using DMSO rather than glycerol. The viability of goat embryos obtained at the early to hatched blastocyst stages, when collected 7 days after ovulation, was assessed by transferring half the embryos singly to recipients and freezing the remainder before transfer. When pregnancy was tested at the 7th week of gestation, the survival rate of fresh embryos was 67/111 (60%) compared with 64/109 (59%) for frozen-thawed embryos. |
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Palavras-Chave: |
Cryoprotective agents. |
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Thesagro: |
Caprino; Congelamento; Embrião; Reprodução. |
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Thesaurus Nal: |
Blastocyst; Cryopreservation; Dimethyl sulfoxide; Embryology; Glycerol; Goats; Physiology; Reproduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02096naa a2200325 a 4500
001 1522351
005 2023-05-23
008 1990 bl uuuu u00u1 u #d
024 7 $a10.1071/rd9900345.$2DOI
100 1 $aLI, R.
245 $aMaximum survival of frozen goat embryos is attained at the expanded, hatching and hatched blastocyst stages of development.$h[electronic resource]
260 $c1990
520 $aAbstract: Embryos were collected 5, 6 and 7 days after ovulation to determine their optimum stage of development for cryopreservation. The embryos were frozen using either glycerol or dimethyl sulfoxide (DMSO) as cryoprotectant, and then thawed and cultured to determine their viability. The rate of survival increased as the stage of development of the embryos increased. All hatched blastocysts survived freezing regardless of which cryoprotectant was used, whereas none of the 22 morulae frozen with DMSO survived, and only 12 of the 63 morulae frozen with glycerol survived. At the early blastocyst stage there was a significantly different result between freezing procedures (P less than 0.05) with greater survival rates obtained using DMSO rather than glycerol. The viability of goat embryos obtained at the early to hatched blastocyst stages, when collected 7 days after ovulation, was assessed by transferring half the embryos singly to recipients and freezing the remainder before transfer. When pregnancy was tested at the 7th week of gestation, the survival rate of fresh embryos was 67/111 (60%) compared with 64/109 (59%) for frozen-thawed embryos.
650 $aBlastocyst
650 $aCryopreservation
650 $aDimethyl sulfoxide
650 $aEmbryology
650 $aGlycerol
650 $aGoats
650 $aPhysiology
650 $aReproduction
650 $aCaprino
650 $aCongelamento
650 $aEmbrião
650 $aReprodução
653 $aCryoprotective agents
700 1 $aCAMERON, W. N.
700 1 $aBATT, P. A.
700 1 $aTROUNSON, O. A.
773 $tReproduction, Fertility and Development$gv. 2, n. 4, p. 345-350, 1990.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 2. |  | MOREIRA, A. A.; HILSDORF, A. W. S.; SILVA, J. V. da; SOUZA, V. R. de. Variabilidade genética de duas variedades de tilápia nilótica por meio de marcadores microssatélites Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 4, p. 521-526, abr. 2007 Título em inglês: Genetic variability of two Nile tilapia strains by microsatellites markers.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | OLIVEIRA, T.; SOUZA, V. R. de; MONTENEGRO, J.; ABREU, J. P. de; FRAUCHES, N.; TEODORO, A. J.; FREITAS-SILVA, O. Characterization of pulp powder of Murici (Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC.) and Taperebá (Spondias mombin) for use as functional food. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS , 12., 2017, Campinas. Ciência de Alimentos e seu Impacto no Mundo em Transformação. Campinas: UNICAMP, 2017. SLACA, 12. De 4 a 7 de Novembro de 2017. Ref. 70998.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 5. | | SOUZA, V. R. de; BRUM, M. C. M.; GUIMARÃES, I. dos S.; SANTOS, P. de F.; AMARAL, T. O. do; ABREU, J. P.; PASSOS, T.; FREITAS-SILVA, O.; GIMBA, E. R. P.; TEODORO, A. J. Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines. Biomolecules, v. 9, n. 11, art. 707, p. 2-15 , 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 6. | | FERNANDES, F. E. P.; PAIVA, A. D. R.; SOUSA, M. G. da S.; CASTRO, M. M.; SOUZA, V. R. de; TOMAZ, T. K. L.; SILVA, A. C.; FERNANDES, C. de S.; BUSSONS, N. de L. Rede de produção e comercialização de alimentos saudáveis: sistematização de experiências no âmbito do projeto Redinovagroeco. Sobral: Embrapa Caprinos e Ovinos, 2022. 40 p. (Embrapa Caprinos e Ovinos. Documentos, 152). ODS 1; ODS 2; ODS 5; ODS 12.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | SOUZA, V. R. de; ANICETO A.; ABREU, J. P.; MONTENEGRO, J.; BOQUIMPANI, B.; JESUZ, B. A. de; CAMPOS, M. de B. E.; MARCELLINI, P. S.; FREITAS-SILVA, O.; CADENA, R.; TEODORO, A. J. Fruit-based drink sensory, physicochemical, and antioxidant properties in the Amazon region: Murici (Byrsonima crassifolia (L.) Kunth and verbascifolia (L.) DC) and tapereba (Spondia mombin). Food Science and Nutrition, v, 8, n. 5, p. 2341-2347, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 7 | |
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