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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
07/08/2025 |
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Data da última atualização: |
03/11/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, L. P.; MARTINHÃO, L. A. G.; GRAZIA, J. G. V.; FARIA, O. A. C.; FIGUEIREDO, R. A.; VIANA, J. H. M. |
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Afiliação: |
LETICIA PRATES MARTINS, UNIVERSIDADE DE BRASÍLIA; LUANY ALVES GALVÃO MARTINHÃO, UNIVERSIDADE DE BRASÍLIA; JOÃO GABRIEL VIANA GRAZIA, FIVX APOYAR BIOTECH; OTAVIO AUGUSTO COSTA FARIA, UNIVERSIDADE DE BRASÍLIA; RICARDO ALAMINO FIGUEIREDO, CENARGEN; JOAO HENRIQUE MOREIRA VIANA, CENARGEN. |
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Título: |
In vitro maturation of Nelore breed (Bos taurus indicus) oocytes using either purified porcine FSH or recombinant human FSH: results from a large-scale in vitro embryo production routine. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Frontiers in Veterinary Science, v. 12, 1596006, 2025. |
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DOI: |
https://doi.org/10.3389/fvets.2025.1596006 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction: This study evaluated whether porcine FSH (pFSH) could be replaced by follitropin-alpha, a recombinant human FSH (rhFSH), during in vitro maturation (IVM) of cumulus-oocyte complexes (COC) recovered from Nelore (Bos taurus indicus) cattle in a large-scale in vitro embryo production (IVP) program. Methods: We performed three experiments, all using grade I COC (n = 10,208) submitted to IVM in TCM199 without FSH (-FSH, negative control) or supplemented with either 0.5 μg/mL pFSH (Folltropin-V) or 0.1 IU rhFSH (Gonal-F). The remaining procedures, media, and culture conditions for in vitro embryo production (IVP) were similar. In Exp. 1, COC (n = 2,791) were IVM in the absence of FSH (-FSH) or with pFSH or rhFSH, and underwent IVP. Blastocysts were assessed for hatching as fresh or after vitrification and warming. In Exp. 2, COC (n = 720) were IVM in groups -FSH, pFSH, or rhFSH, and the expanded blastocysts produced were stained with Hoechst 33342 and propidium iodide for total, trophoblast and ICM cell count. In Exp. 3, we used the same experimental design as in Exp. 1 but in a commercial IVP routine, which included the use of COC (n = 6,697) recovered both by OPU and from slaughterhouse, Y-sorted semen, and the transfer of part of the blastocysts produced. Results: In Exp. 1, blastocyst rates were greater (p < 0.05) in groups treated with than without FSH (39.3% and 40.6% vs 34.8% for pFSH, rhFSH and -FSH, respectively; p = 0.0244). However, there was no effect of treatment on hatching rates of fresh (74.5%, 79.0% and 70.1% for -FSH, pFSH, and rhFSH, respectively; p = 0.2621) or vitrified blastocysts (75.0%, 69.6% and 73.2% for -FSH, pFSH, and rhFSH, respectively; p = 0.7623). In Exp. 2, blastocysts from the rhFSH group presented more cells in the trophoblast (110.3±5.6 vs 80.0±5.7 and 878.4±5.1 for rhFSH, -FSH, and pFSH, respectively; P=0.0002), but not in the ICM (p = 0.3231), when compared with pFSH and -FSH groups. In Exp. 3, both pFSH and rhFSH increased cleavage (76.0% and 75.1% vs 71.6%, p = 0.0035) and blastocyst rates (38.1% and 39.5% vs 34.3%, p = 0.0102), but not pregnancy rate (43.8% and 33.3% vs 56.0%, p = 0.1080), compared with -FSH. Discussion: In summary, follitropin-alpha is an alternative to pFSH as a medium supplement for IVM of bovine COC. MenosIntroduction: This study evaluated whether porcine FSH (pFSH) could be replaced by follitropin-alpha, a recombinant human FSH (rhFSH), during in vitro maturation (IVM) of cumulus-oocyte complexes (COC) recovered from Nelore (Bos taurus indicus) cattle in a large-scale in vitro embryo production (IVP) program. Methods: We performed three experiments, all using grade I COC (n = 10,208) submitted to IVM in TCM199 without FSH (-FSH, negative control) or supplemented with either 0.5 μg/mL pFSH (Folltropin-V) or 0.1 IU rhFSH (Gonal-F). The remaining procedures, media, and culture conditions for in vitro embryo production (IVP) were similar. In Exp. 1, COC (n = 2,791) were IVM in the absence of FSH (-FSH) or with pFSH or rhFSH, and underwent IVP. Blastocysts were assessed for hatching as fresh or after vitrification and warming. In Exp. 2, COC (n = 720) were IVM in groups -FSH, pFSH, or rhFSH, and the expanded blastocysts produced were stained with Hoechst 33342 and propidium iodide for total, trophoblast and ICM cell count. In Exp. 3, we used the same experimental design as in Exp. 1 but in a commercial IVP routine, which included the use of COC (n = 6,697) recovered both by OPU and from slaughterhouse, Y-sorted semen, and the transfer of part of the blastocysts produced. Results: In Exp. 1, blastocyst rates were greater (p < 0.05) in groups treated with than without FSH (39.3% and 40.6% vs 34.8% for pFSH, rhFSH and -FSH, respectively; p = 0.0244). However, there was no effect of ... Mostrar Tudo |
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Palavras-Chave: |
Cumulus-oocyte complexes; In vitro maturation; Recombinant hormones. |
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Thesaurus Nal: |
Cattle; Gonadotropins. |
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Categoria do assunto: |
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Marc: |
LEADER 03219naa a2200253 a 4500
001 2177781
005 2025-11-03
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fvets.2025.1596006$2DOI
100 1 $aMARTINS, L. P.
245 $aIn vitro maturation of Nelore breed (Bos taurus indicus) oocytes using either purified porcine FSH or recombinant human FSH$bresults from a large-scale in vitro embryo production routine.$h[electronic resource]
260 $c2025
520 $aIntroduction: This study evaluated whether porcine FSH (pFSH) could be replaced by follitropin-alpha, a recombinant human FSH (rhFSH), during in vitro maturation (IVM) of cumulus-oocyte complexes (COC) recovered from Nelore (Bos taurus indicus) cattle in a large-scale in vitro embryo production (IVP) program. Methods: We performed three experiments, all using grade I COC (n = 10,208) submitted to IVM in TCM199 without FSH (-FSH, negative control) or supplemented with either 0.5 μg/mL pFSH (Folltropin-V) or 0.1 IU rhFSH (Gonal-F). The remaining procedures, media, and culture conditions for in vitro embryo production (IVP) were similar. In Exp. 1, COC (n = 2,791) were IVM in the absence of FSH (-FSH) or with pFSH or rhFSH, and underwent IVP. Blastocysts were assessed for hatching as fresh or after vitrification and warming. In Exp. 2, COC (n = 720) were IVM in groups -FSH, pFSH, or rhFSH, and the expanded blastocysts produced were stained with Hoechst 33342 and propidium iodide for total, trophoblast and ICM cell count. In Exp. 3, we used the same experimental design as in Exp. 1 but in a commercial IVP routine, which included the use of COC (n = 6,697) recovered both by OPU and from slaughterhouse, Y-sorted semen, and the transfer of part of the blastocysts produced. Results: In Exp. 1, blastocyst rates were greater (p < 0.05) in groups treated with than without FSH (39.3% and 40.6% vs 34.8% for pFSH, rhFSH and -FSH, respectively; p = 0.0244). However, there was no effect of treatment on hatching rates of fresh (74.5%, 79.0% and 70.1% for -FSH, pFSH, and rhFSH, respectively; p = 0.2621) or vitrified blastocysts (75.0%, 69.6% and 73.2% for -FSH, pFSH, and rhFSH, respectively; p = 0.7623). In Exp. 2, blastocysts from the rhFSH group presented more cells in the trophoblast (110.3±5.6 vs 80.0±5.7 and 878.4±5.1 for rhFSH, -FSH, and pFSH, respectively; P=0.0002), but not in the ICM (p = 0.3231), when compared with pFSH and -FSH groups. In Exp. 3, both pFSH and rhFSH increased cleavage (76.0% and 75.1% vs 71.6%, p = 0.0035) and blastocyst rates (38.1% and 39.5% vs 34.3%, p = 0.0102), but not pregnancy rate (43.8% and 33.3% vs 56.0%, p = 0.1080), compared with -FSH. Discussion: In summary, follitropin-alpha is an alternative to pFSH as a medium supplement for IVM of bovine COC.
650 $aCattle
650 $aGonadotropins
653 $aCumulus-oocyte complexes
653 $aIn vitro maturation
653 $aRecombinant hormones
700 1 $aMARTINHÃO, L. A. G.
700 1 $aGRAZIA, J. G. V.
700 1 $aFARIA, O. A. C.
700 1 $aFIGUEIREDO, R. A.
700 1 $aVIANA, J. H. M.
773 $tFrontiers in Veterinary Science$gv. 12, 1596006, 2025.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 5 | |
| 2. |  | SILVA, R. L. da C.; COSTA, R. S. C. da; PASSOS, A. M. A. dos; LEONIDAS, F. das C.; FIGUEIREDO, L. M.; SOBRAL, I. M. Fungos micorrízicos arbusculares em plantas de cobertura. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 9.; ENCONTRO DE PÓS-GRADUAÇÃO, 4., 2018, Porto Velho. Anais... Porto Velho: Embrapa Rondônia, 2018. p. 37.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 3. | | RAMOS, R. da M.; COSTA, R. S. C. da; PASSOS, A. M. A. dos; AKER, A. M.; SOBRAL, I. M.; LENA, A. G. Fungos micorrízicos arbusculares em plantas de cobertura utilizadas no plantio direto. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 6.; ENCONTRO DE PÓS-GRADUAÇÃO, 2015, Porto Velho, RO. Anais... Porto Velho: Embrapa Rondônia, 2015. p. 88.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 4. | | SOBRAL, I. M.; COSTA, R. S. C. da; PASSOS, A. M. A. dos; LEONIDAS, F. das C.; FIGUEIREDO, L. M.; SILVA, R. L. C. Produtividade de genótipos de mandioca no Município de Ouro Preto do Oeste-RO. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 9.; ENCONTRO DE PÓS-GRADUAÇÃO, 4., 2018, Porto Velho. Anais... Porto Velho: Embrapa Rondônia, 2018. p. 40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 5. | | FIGUEIREDO, L. M.; COSTA, R. S. C. da; LEONIDAS, F. das C.; MENDES, A. M.; SILVA, R. L. C.; SOBRAL, I. M. Ocorrência de micorrizas arbusculares em clones da cultivar Conilon BRS Ouro Preto. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 9.; ENCONTRO DE PÓS-GRADUAÇÃO, 4., 2018, Porto Velho. Anais... Porto Velho: Embrapa Rondônia, 2018. p. 22.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 5 | |
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