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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
14/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BIASE, F. H.; FRANCO, M. M.; GOULART, L. R.; ANTUNES, R. C. |
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Afiliação: |
Fernando Henrique Biase, Universidade Federal de Uberlândia; MAURICIO MACHAIM FRANCO, Cenargen; Luiz Ricardo Goulart, Universidade Federal de Uberlândia; Robson Carlos Antunes, Universidade Federal de Uberlândia. |
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Título: |
Protocol for extraction of genomic DNA from swine solid tissues. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 25, n . 3, p. 313-315, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and amplification of two DNA fragments, 605bp and 891pb, by PCR. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver and adipose tissues presenting the greatest and the smallest concentration, respectively. The described protocol has proven to be advantageous due to its simplicity, quickness, affordable reagents and absence of phenol, resulting in a high molecular weight DNA of good quality from several tissues. |
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Palavras-Chave: |
Swine tissues. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01454naa a2200169 a 4500
001 2067487
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008 2002 bl uuuu u00u1 u #d
100 1 $aBIASE, F. H.
245 $aProtocol for extraction of genomic DNA from swine solid tissues.$h[electronic resource]
260 $c2002
520 $aMolecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and amplification of two DNA fragments, 605bp and 891pb, by PCR. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver and adipose tissues presenting the greatest and the smallest concentration, respectively. The described protocol has proven to be advantageous due to its simplicity, quickness, affordable reagents and absence of phenol, resulting in a high molecular weight DNA of good quality from several tissues.
653 $aSwine tissues
700 1 $aFRANCO, M. M.
700 1 $aGOULART, L. R.
700 1 $aANTUNES, R. C.
773 $tGenetics and Molecular Biology$gv. 25, n . 3, p. 313-315, 2002.
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