| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pantanal. |
|
Data corrente: |
08/06/2017 |
|
Data da última atualização: |
19/12/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LEÃO, B. C. S.; FRIGONI, N. A. S. R.; NOGUEIRA, E.; CABRAL, E. C.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NEVES, T. V.; MINGOTI, G. Z. |
|
Afiliação: |
BEATRIZ C. S. LEÃO, Universidade Estadual Paulista; NATHÁLIA A. S. ROCHA FRIGONI, Universidade Estadual Paulista; ERIKLIS NOGUEIRA, CPAP; ELAINE C. CABRAL, ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas; CHRISTINA R. FERREIRA, ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas; MARCOS N. EBERLIN, ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas; MÔNICA F. ACCORSI, Universidade Estadual Paulista; THIAGO V. NEVES, Universidade Estadual Paulista; GISELE Z. MINGOTI, Universidade Estadual Paulista. |
|
Título: |
Membrane lipid profile of in vitro-produced embryos is affected by vitrification but not by long-term dietary supplementation of polyunsaturated fatty acids for oocyte donor beef heifers. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 29, n.6, p. 1217-1230, 2017. |
|
DOI: |
10.1071/RD15414 |
|
Idioma: |
Inglês |
|
Conteúdo: |
ABSTRACT: Dietary rumen-protected polyunsaturated fatty acids (PUFAs) rich in linoleic acid (LA) may affect embryo yield, and LA can modulate the molecular mechanisms of lipid uptake in bovine blastocysts produced in vitro. In embryos, membrane lipids, such as phosphatidylcholines (PCs) and sphingomyelins (SMs), affect cryopreservation success. The aim of the present study was to evaluate embryonic developmental rates after the IVF of oocytes retrieved from Nellore heifers fed for approximately 90 days with rumen-protected PUFAs rich in LA. In addition, we evaluated embryo cryotolerance and the membrane structure lipid composition using matrix-assisted laser desorption ionisation mass spectrometry of fresh and vitrified embryos. Embryo development to the blastocyst stage (mean 43.2%) and embryo survival after vitrification and warming (mean 79.3%) were unaffected by diet. The relative abundance of one lipid species (PC ether (PCe; 38:2, which means that this lipid has 38 carbon atoms and 2 double bonds in the fatty acyl residues) was increased after PUFAs supplementation. However, 10 ions were affected by cryopreservation; ions consistent with PC 32:0, PC 34:1, SM 24:1, PC 40:6 or PC 42:9, PC plasmalogen (PCp) 44:10 or PC 42:7, triacylglycerol (TAG) 54:9 and a not assigned ion (m/z 833.2) were lower in blastocysts that survived to the cryopreservation process compared with fresh blastocysts, whereas the abundance of the ions PC 36:3 or PC 34:0, PCe 38:2 or PC 36:6 and PC 36:5 or PCe 38:1 were increased after cryopreservation. Thus, the results demonstrate that the mass spectrometry profiles of PC, SM and TAG species differ significantly in bovine blastocysts upon cryopreservation. Because the lipid ion abundances of fresh and vitrified?warmed embryos were distinct, they can be used as potential markers of post-cryopreservation embryonic survival. MenosABSTRACT: Dietary rumen-protected polyunsaturated fatty acids (PUFAs) rich in linoleic acid (LA) may affect embryo yield, and LA can modulate the molecular mechanisms of lipid uptake in bovine blastocysts produced in vitro. In embryos, membrane lipids, such as phosphatidylcholines (PCs) and sphingomyelins (SMs), affect cryopreservation success. The aim of the present study was to evaluate embryonic developmental rates after the IVF of oocytes retrieved from Nellore heifers fed for approximately 90 days with rumen-protected PUFAs rich in LA. In addition, we evaluated embryo cryotolerance and the membrane structure lipid composition using matrix-assisted laser desorption ionisation mass spectrometry of fresh and vitrified embryos. Embryo development to the blastocyst stage (mean 43.2%) and embryo survival after vitrification and warming (mean 79.3%) were unaffected by diet. The relative abundance of one lipid species (PC ether (PCe; 38:2, which means that this lipid has 38 carbon atoms and 2 double bonds in the fatty acyl residues) was increased after PUFAs supplementation. However, 10 ions were affected by cryopreservation; ions consistent with PC 32:0, PC 34:1, SM 24:1, PC 40:6 or PC 42:9, PC plasmalogen (PCp) 44:10 or PC 42:7, triacylglycerol (TAG) 54:9 and a not assigned ion (m/z 833.2) were lower in blastocysts that survived to the cryopreservation process compared with fresh blastocysts, whereas the abundance of the ions PC 36:3 or PC 34:0, PCe 38:2 or PC 36:6 and PC 36:... Mostrar Tudo |
|
Thesagro: |
Bovino; Embrião animal. |
|
Thesaurus Nal: |
Blastocyst; Cattle; Mass spectrometry. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02812naa a2200289 a 4500
001 2070647
005 2022-12-19
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1071/RD15414$2DOI
100 1 $aLEÃO, B. C. S.
245 $aMembrane lipid profile of in vitro-produced embryos is affected by vitrification but not by long-term dietary supplementation of polyunsaturated fatty acids for oocyte donor beef heifers.$h[electronic resource]
260 $c2017
520 $aABSTRACT: Dietary rumen-protected polyunsaturated fatty acids (PUFAs) rich in linoleic acid (LA) may affect embryo yield, and LA can modulate the molecular mechanisms of lipid uptake in bovine blastocysts produced in vitro. In embryos, membrane lipids, such as phosphatidylcholines (PCs) and sphingomyelins (SMs), affect cryopreservation success. The aim of the present study was to evaluate embryonic developmental rates after the IVF of oocytes retrieved from Nellore heifers fed for approximately 90 days with rumen-protected PUFAs rich in LA. In addition, we evaluated embryo cryotolerance and the membrane structure lipid composition using matrix-assisted laser desorption ionisation mass spectrometry of fresh and vitrified embryos. Embryo development to the blastocyst stage (mean 43.2%) and embryo survival after vitrification and warming (mean 79.3%) were unaffected by diet. The relative abundance of one lipid species (PC ether (PCe; 38:2, which means that this lipid has 38 carbon atoms and 2 double bonds in the fatty acyl residues) was increased after PUFAs supplementation. However, 10 ions were affected by cryopreservation; ions consistent with PC 32:0, PC 34:1, SM 24:1, PC 40:6 or PC 42:9, PC plasmalogen (PCp) 44:10 or PC 42:7, triacylglycerol (TAG) 54:9 and a not assigned ion (m/z 833.2) were lower in blastocysts that survived to the cryopreservation process compared with fresh blastocysts, whereas the abundance of the ions PC 36:3 or PC 34:0, PCe 38:2 or PC 36:6 and PC 36:5 or PCe 38:1 were increased after cryopreservation. Thus, the results demonstrate that the mass spectrometry profiles of PC, SM and TAG species differ significantly in bovine blastocysts upon cryopreservation. Because the lipid ion abundances of fresh and vitrified?warmed embryos were distinct, they can be used as potential markers of post-cryopreservation embryonic survival.
650 $aBlastocyst
650 $aCattle
650 $aMass spectrometry
650 $aBovino
650 $aEmbrião animal
700 1 $aFRIGONI, N. A. S. R.
700 1 $aNOGUEIRA, E.
700 1 $aCABRAL, E. C.
700 1 $aFERREIRA, C. R.
700 1 $aEBERLIN, M. N.
700 1 $aACCORSI, M. F.
700 1 $aNEVES, T. V.
700 1 $aMINGOTI, G. Z.
773 $tReproduction, Fertility and Development$gv. 29, n.6, p. 1217-1230, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pantanal (CPAP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 14 | |
| 11. |  | SANTOS, V. da S.; RANGEL, M. A. S.; RINGENBERG, R.; OLIVEIRA, L. A. de; OTSUBO, A. A.; FERNANDES, A. M.; LEONEL, M.; SILVEIRA, H. F. da. BRS Boitatá and BRS Ocauçu: cassava cultivars for the industry. Crop Breeding and Applied Biotechnology, v. 24, n. 2, e47822425, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Mandioca e Fruticultura. |
|    |
| 12. | | HORA, R. L A. C. da; SOUZA, A. da S.; SANTOS, K. C. F. dos; SILVEIRA, H. F. da; ROCHA, H. S. Micropropagação da mandioca (Manihot esculenta Crantz) : uma técnica consolidada para a multiplicação in vitro de variedades. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 13. | | ROCHA, H. S.; ARAUJO, J. C. de; SILVA, A. C. M. da; OLIVEIRA, S. A. S. de; BORGES, A. L.; FERREIRA FILHO, J. R.; MEISSNER FILHO, P. E.; SILVEIRA, H. F. da; RINGENBERG, R.; CARDOSO, C. E. L. Recomendações técnicas para a produção de manivas-semente de mandioca a partir de mudas micropropagadas. O papel do "maniveiro" - Projeto RENIVA. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2014.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 14. | | ROCHA, H. S.; SILVEIRA, H. F. da; SOUZA, A. da S.; OLIVEIRA, S. A. S. de; OLIVEIRA, E. J. de; SOUZA, B. D. L. de; GONÇALVES, H. A.; ACHECO JÚNIOR, R. G. P.; ALMEIDA, N. M. de; LIMA, S. A. de L. Miniestacas de mandioca - nova alternativa de material de plantio. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2021. (Embrapa Mandioca e Fruticultura. Comunicado Técnico, 179).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| Registros recuperados : 14 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
