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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
08/11/2024 |
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Data da última atualização: |
08/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, I. C. B.; CARVALHO, A. M. S.; WENDLAND, A.; ROSSATO, M. |
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Afiliação: |
IAN C. BISPO CARVALHO, UNIVERSIDADE DE BRASÍLIA; ALICE MARIA SILVA CARVALHO, UNIVERSIDADE DE BRASÍLIA; ADRIANE WENDLAND FERREIRA, CNPAF; MAURICIO ROSSATO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Colorimetric LAMP assay for detection of Xanthomonas phaseoli pv. manihotis in cassava through genomics: a new approach to an old problem. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Plant Disease, v. 108, n. 10, p. 2993-3000, Oct. 2024. |
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ISSN: |
1943-7692 |
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DOI: |
https://doi.org/10.1094/PDIS-08-23-1507-RE |
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Idioma: |
Inglês |
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Conteúdo: |
Bacterial blight caused by Xanthomonas phaseoli pv. manihotis (Xpm) is considered the main bacterial disease that affects cassava, causing significant losses when not properly managed. In the present study, a fast, sensitive, and easy-to-apply method to detect Xpm via colorimetric loop-mediated isothermal amplification (LAMP) was developed. To ensure the use of a unique-to-the-target pathovar core region for primer design, 74 complete genomic sequences of Xpm together with different bacterial species and pathovars were used for comparative genomics. A total of 42 unique genes were used to design 27 LAMP primer sets, from which nine primers were synthesized, and only one (Xpm_Lp1 primer set) showed sufficient efficiency in preliminary tests. The sensitivity, assessed by a serial dilution of the type strain (IBSBF 278) DNA, yielded high sensitivity, detecting up to 100 fg. The LAMP primers showed high specificity, did not cross-react with other bacterial species or other pathovars tested, and amplified only the Xpm isolates. Tests confirmed the high efficiency of the protocol using infected or inoculated macerated cassava leaves without the need for additional sample treatment. The LAMP test developed in this study was able to detect Xpm in a fast, simple, and sensitive way, and it can be used to monitor the disease under laboratory and field conditions. |
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Palavras-Chave: |
Bacterial blight; Xanthomonas phaseoli pv manihotis. |
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Thesagro: |
Mandioca; Xanthomonas Phaseoli. |
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Thesaurus Nal: |
Bacterial diseases of plants; Cassava; Comparative genomic hybridization; Genomics; Loop-mediated isothermal amplification. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02344naa a2200289 a 4500
001 2168988
005 2024-11-08
008 2024 bl uuuu u00u1 u #d
022 $a1943-7692
024 7 $ahttps://doi.org/10.1094/PDIS-08-23-1507-RE$2DOI
100 1 $aCARVALHO, I. C. B.
245 $aColorimetric LAMP assay for detection of Xanthomonas phaseoli pv. manihotis in cassava through genomics$ba new approach to an old problem.$h[electronic resource]
260 $c2024
520 $aBacterial blight caused by Xanthomonas phaseoli pv. manihotis (Xpm) is considered the main bacterial disease that affects cassava, causing significant losses when not properly managed. In the present study, a fast, sensitive, and easy-to-apply method to detect Xpm via colorimetric loop-mediated isothermal amplification (LAMP) was developed. To ensure the use of a unique-to-the-target pathovar core region for primer design, 74 complete genomic sequences of Xpm together with different bacterial species and pathovars were used for comparative genomics. A total of 42 unique genes were used to design 27 LAMP primer sets, from which nine primers were synthesized, and only one (Xpm_Lp1 primer set) showed sufficient efficiency in preliminary tests. The sensitivity, assessed by a serial dilution of the type strain (IBSBF 278) DNA, yielded high sensitivity, detecting up to 100 fg. The LAMP primers showed high specificity, did not cross-react with other bacterial species or other pathovars tested, and amplified only the Xpm isolates. Tests confirmed the high efficiency of the protocol using infected or inoculated macerated cassava leaves without the need for additional sample treatment. The LAMP test developed in this study was able to detect Xpm in a fast, simple, and sensitive way, and it can be used to monitor the disease under laboratory and field conditions.
650 $aBacterial diseases of plants
650 $aCassava
650 $aComparative genomic hybridization
650 $aGenomics
650 $aLoop-mediated isothermal amplification
650 $aMandioca
650 $aXanthomonas Phaseoli
653 $aBacterial blight
653 $aXanthomonas phaseoli pv manihotis
700 1 $aCARVALHO, A. M. S.
700 1 $aWENDLAND, A.
700 1 $aROSSATO, M.
773 $tPlant Disease$gv. 108, n. 10, p. 2993-3000, Oct. 2024.
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