| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
|
Data corrente: |
20/09/2017 |
|
Data da última atualização: |
08/03/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, M. B.; CARVALHO, C. W. P. de; GARCIA-ROJAS, E. E. |
|
Afiliação: |
MONIQUE BARRETO SANTOS, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; CARLOS WANDERLEI PILER DE CARVALHO, CTAA; EDWIN ELARD GARCIA-ROJAS, UNIVERSIDADE FEDERAL FLUMINENSE. |
|
Título: |
Heteroprotein complex formation of bovine serum albumin and lysozyme: Structure and thermal stability. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Food Hydrocolloids, n. 74, p. 267-274, 2018. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The formation of a heteroprotein complex obtained by the interaction of bovine serum albumin (BSA) and lysozyme (Lys) was investigated by pH variation using turbidimetric analysis and zeta potential (ζ) at different protein ratios and NaCl concentrations. The complexes were formed in a pH range between 8.0 and 11.0, with the ratio r = 0.5 at pH 9.0 presenting the highest complexation. The addition of NaCl decreased the interaction at concentrations of 10 mM. The complex formation occurred between the isoelectric points (pI) of the proteins, close to a balance of charges, mainly by electrostatic interactions with some participation of hydrogen bonds. Differential scanning calorimetry suggested that the interaction gave rise to a new biopolymer due to the formation of a single denaturation point at 67 °C. The structures formed had an average size of ∼1.7 μm, well above that of the isolated proteins, and microscopic analysis revealed that the complexes had a globular structure. BSA/Lys complexes may be a potential bioactive encapsulating agent and may be used as a food ingredient. |
|
Palavras-Chave: |
Coacervation; Differential scanning; Electrostatic interaction; Globular proteins; Microencapsulating. |
|
Thesaurus Nal: |
Calorimetry. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 01772naa a2200217 a 4500
001 2075911
005 2024-03-08
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. B.
245 $aHeteroprotein complex formation of bovine serum albumin and lysozyme$bStructure and thermal stability.$h[electronic resource]
260 $c2018
520 $aThe formation of a heteroprotein complex obtained by the interaction of bovine serum albumin (BSA) and lysozyme (Lys) was investigated by pH variation using turbidimetric analysis and zeta potential (ζ) at different protein ratios and NaCl concentrations. The complexes were formed in a pH range between 8.0 and 11.0, with the ratio r = 0.5 at pH 9.0 presenting the highest complexation. The addition of NaCl decreased the interaction at concentrations of 10 mM. The complex formation occurred between the isoelectric points (pI) of the proteins, close to a balance of charges, mainly by electrostatic interactions with some participation of hydrogen bonds. Differential scanning calorimetry suggested that the interaction gave rise to a new biopolymer due to the formation of a single denaturation point at 67 °C. The structures formed had an average size of ∼1.7 μm, well above that of the isolated proteins, and microscopic analysis revealed that the complexes had a globular structure. BSA/Lys complexes may be a potential bioactive encapsulating agent and may be used as a food ingredient.
650 $aCalorimetry
653 $aCoacervation
653 $aDifferential scanning
653 $aElectrostatic interaction
653 $aGlobular proteins
653 $aMicroencapsulating
700 1 $aCARVALHO, C. W. P. de
700 1 $aGARCIA-ROJAS, E. E.
773 $tFood Hydrocolloids$gn. 74, p. 267-274, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 6 | |
| 2. |  | SCHWAB, N. T.; STRECK, N. A.; LANGNER, J. A.; RIBEIRO, B. S. M. R.; UHLMANN, L. O.; BECKER, C. C. Aplicabilidade do termo antocrono para representar a velocidade de abertura de flores em inflorescência. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 9, p. 657-664, set. 2014. Título em inglês: Biology and fertility life table of grapevine aphid on grape cultivars.| Biblioteca(s): Embrapa Unidades Centrais. |
|    |
| 3. | | UHLMANN, L. O.; STRECK, N. A.; BECKER, C. C.; TOMIOZZO, R.; SCHWAB, N. T.; ORTIZ, V. M. Climate risk zoning for gladiolus in the state of Rio Grande do Sul, Brazil. Pesquisa Agropecuária Brasileira, v. 55, e01094, 2020. Título em português: Zoneamento de risco climático para o gladíolo no estado do Rio Grande do Sul.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 4. | | ABDELNUR, P. V.; SARAIVA, S. A.; CATHARINO, R. R.; COELHO, M.; SCHWAB, N.; GARCIA, C. M.; SCHUCHARDT, U.; SOUZA, V.; EBERLIN, M. N. Blends of soybean biodiesel with petrodiesel: direct quantitation via mass spectrometry. Journal of the Brazilian Chemical Society, v. 4, n. 6, p. 946-952, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
|  |
| 5. | | SCHWAB, N. T.; STRECK, N. A.; RIBEIRO, B. S. M. R.; BECKER, C. C.; LANGNER, J. A.; UHLMANN, L. O.; RIBAS, G. G. Parâmetro quantitativos de hastes florais de gladíolo conforme a data de plantio em ambiente subtropical. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 10, p. 902-911, out. 2015. Título em inglês: Quantitative parameters of gladiolus stems according to planting dates in a subtropical environment.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 6. | | ROSA, J. S. da; FREITAS-SILVA, O.; ROUWS, J. R. C.; MOREIRA, I. G. da S.; NOVAES, F. J. M.; AZEVEDO, D. de A.; SCHWAB, N.; GODOY, R. L. de O.; EBERLIN, M. N.; REZENDE, C. M. de. Mass spectrometry screening of Arabica coffee roasting: A non-target and non-volatile approach by EASI-MS and ESI-MS. Food Research International [online], v. 89, part 2, p. 967-975, nov. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Agroindústria de Alimentos. |
| |
| Registros recuperados : 6 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
