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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
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Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
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Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
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Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus Nal: |
Vaccines. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| 1. |  | BUSS, G. L.; SONCINI, M. M.; SEHN, C. F. S.; ROSA, C. M. da; SCIVITTARO, W. B.; SAOUSA, R. O de. Influência de sistemas de irrigação sobre a emissão de metano e de óxido nitroso em solo cultivado com arroz. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 4., 2012, Pelotas. Ciência e inovação para 2050: qual o futuro que queremos? Resumos e palestras... Pelotas: Embrapa Clima Temperado, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
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| 2. | | VEÇOZZI, T. A.; SAOUSA, R. O. de; SCIVITTARO, W. B.; SILVEIRA, A. D.; FARIAS, M. de O.; RODRIGUES, M.; WOLTER, R. C. D. Uso de ureia e de fertilizante nitrogenado de liberação lenta no cultivo de arroz irrigado: efeito nas emissões de CH4 do solo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 31.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 15.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 10., 2014, Araxá. Fertilidade e biologia do solo: integração e tecnologias para todos: anais. Araxá: Núcleo Regional Leste da Sociedade Brasileira de Ciência do Solo, 2014. FertBio 2014.| Tipo: Artigo em Anais de Congresso |
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