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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/04/2024 |
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Data da última atualização: |
11/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, E. R. dos; CAMARGO, B. R. de; SILVA, L. A. da; LAUMANN, R. A.; RIBEIRO, B. M.; ARDISSON-ARAÚJO, D. M. P. |
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Afiliação: |
ETHIANE ROZO DOS SANTOS, UNIVERSIDADE DE BRASÍLIA; BRENDA RABELO DE CAMARGO, UNIVERSIDADE DE BRASÍLIA; LEONARDO ASSIS DA SILVA, UNIVERSIDADE DE BRASÍLIA; RAUL ALBERTO LAUMANN, CENARGEN; BERGMANN MORAIS RIBEIRO, UNIVERSIDADE DE BRASÍLIA; DANIEL M. P. ARDISSON-ARAÚJO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae). |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 55, p. 1913-1921, 2024. |
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DOI: |
https://doi.org/10.1007/s42770-024-01340-y |
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Idioma: |
Português |
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Conteúdo: |
Wasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. MenosWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological si... Mostrar Tudo |
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Palavras-Chave: |
Egg parasitoid; Halyomorpha halys virus; Stinkbug; Telenomus podisi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02548naa a2200241 a 4500
001 2163741
005 2024-06-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-024-01340-y$2DOI
100 1 $aSANTOS, E. R. dos
245 $aThe multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera$bPlatygastridae).$h[electronic resource]
260 $c2024
520 $aWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector.
653 $aEgg parasitoid
653 $aHalyomorpha halys virus
653 $aStinkbug
653 $aTelenomus podisi
700 1 $aCAMARGO, B. R. de
700 1 $aSILVA, L. A. da
700 1 $aLAUMANN, R. A.
700 1 $aRIBEIRO, B. M.
700 1 $aARDISSON-ARAÚJO, D. M. P.
773 $tBrazilian Journal of Microbiology$gv. 55, p. 1913-1921, 2024.
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