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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/07/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENEZES, S. P.; SILVA, D. M. de A.; LIMA, E. M.; SOUSA, A. O. de; ANDRADE, B. S.; LEMOS, L. S. L.; GRAMACHO, A. P.; GESTEIRA, A. da S.; PIROVANI, C. P.; MICHELI, F. |
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Afiliação: |
SARA PEREIRA MENEZES, UESC; DSON MARIO DE ANDRADE SILVA, UESC; ELINE MATOS LIMA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; BRUNO SILVA ANDRADE; LIVIA SANTOS LIMA LEMOS; ARINA PERES GRAMACHO; ABELMON DA SILVA GESTEIRA, CNPMF; CARLOS PRIMINHO PIROVANI; FABIENNE MICHELI, UESC. |
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Título: |
The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora perniciosa. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Plant Biology, 2014. |
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ISSN: |
1471-2229 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. MenosBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first rep... Mostrar Tudo |
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Thesagro: |
Cacau; Theobroma Cacao. |
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Categoria do assunto: |
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Marc: |
LEADER 02704naa a2200265 a 4500
001 1991551
005 2023-05-25
008 2014 bl uuuu u00u1 u #d
022 $a1471-2229
100 1 $aMENEZES, S. P.
245 $aThe pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora perniciosa.$h[electronic resource]
260 $c2014
520 $aBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively.
650 $aCacau
650 $aTheobroma Cacao
700 1 $aSILVA, D. M. de A.
700 1 $aLIMA, E. M.
700 1 $aSOUSA, A. O. de
700 1 $aANDRADE, B. S.
700 1 $aLEMOS, L. S. L.
700 1 $aGRAMACHO, A. P.
700 1 $aGESTEIRA, A. da S.
700 1 $aPIROVANI, C. P.
700 1 $aMICHELI, F.
773 $tBMC Plant Biology, 2014.
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| 4. |  | TRAJANO, E. V. de A.; DRUMOND, M. A.; SANTOS, Y. M. dos; SANTOS, R. V. dos. Aproveitamento de rejeitos da mineração de caulim e vermiculita na produção de mudas de pinhão-manso no Sertão paraibano. In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO MANSO, 1., 2012, Guarapari. Desafios e oportunidades: anais... Campina grande: Embrapa Algodão, 2012. p. 262. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
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| 8. | | SANTOS, R. V. dos; FOGACA, F. H. dos S.; PEREIRA, A. M. L.; MIZAEL, F. H.; RODRIGUES, L. A.; MAGALHAES, J. A. Depuration of Anomalocardia brasiliana to increase the value of shellfish collected on the Piaui Coast, Brazil. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 18., 2016. Dublin. Greening the global food supply Chain: through innovation in food science and technology: Book of Proceedings. Dublin: IFSTI, 2016. IUFOST 2016.| Tipo: Resumo em Anais de Congresso |
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| 9. | | SANTOS, G. R. O. dos; SANTOS, R. V. dos; VITAL, A. de F. M.; FARIAS JR. J. A.; ARAÚJO, J. M.; TRAJANO, E. V. de A. Crescimento inicial da mamoneira (ricinus communis l.) em áreas degradadas do Semi-Árido. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.| Biblioteca(s): Embrapa Algodão. |
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| 10. | | NOGUEIRA, E. de M.; VINAGRE, F.; MASUDA, H. P.; VARGAS, C.; PÁDUA, V. L. M.; SILVA, F. R. da; SANTOS, R. V. dos; BALDANI, J. I.; FERREIRA, P. C. G.; HEMERLY, A. S. Expression of sugarcane ganes induced by inoculation with Gluconacetobacter diazotrophicus and Herbaspirillum rubrisubalbicans. Genetics and Molecular Biology, Ribeirão Preto, v. 24, n. 1-4, p. 199-206, jan./dec. 2001.| Biblioteca(s): Embrapa Agrobiologia. |
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