| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
24/11/2014 |
|
Data da última atualização: |
05/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ROCHA, L. C.; BUSTAMANTE, F. de O.; SILVEIRA, R. A. D. S.; TORRES, G. A.; MITTELMANN, A.; TECHIO, V. H. |
|
Afiliação: |
LAIANE CORSINI ROCHA, UFLA; FERNANDA OLIVEIRA BUSTAMANTE, UFLA; RAPHAELA APARECIDA DUARTE SILVEIRA, UFLA; GIOVANA AUGUSTA TORRES, UFLA; ANDREA MITTELMANN, CNPGL; VANIA HELENA TECHIO, UFLA. |
|
Título: |
Functional repetitive sequences and fragile sites in chromosomes of Lolium perenne L. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Protoplasma, v. 252, n. 2, p. 451-460, 2015. |
|
DOI: |
https://doi.org/10.1007/s00709-014-0690-4 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Lolium perenne is considered a high-quality forage widely used in temperate regions to meet the shortage of forage during the winter. In this species, some peculiarities related to cytogenetic aspects have already been described, as the variability in number and position of 45S ribosomal DNA (rDNA) sites and the expression of fragile sites, which require further studies to support the understanding of their causes and consequences. In this way, this study aimed to evaluate the relationship between the expression of fragile sites and functional repetitive sequences (rDNA and telomeric) in chromosomes of diploid and polyploid cultivars of L. perenne. The techniques of FISH, Ag-NOR and fluorescence banding were used to assess the distribution of sites of 45S rDNA, 5S, telomeric sequences, and the transcriptional activity of the 45S ribosomal genes and the distribution of AT- and/or GC-rich sequences in L. perenne, respectively. There was variability in the number and location of 45S rDNA sites, which was not observed for 5S rDNA sites. One of the genotypes showed two 45S rDNA sites on the same chromosome, located in different chromosome arms. Breaks and gaps were found in 45S rDNA sites in most metaphases evaluated for both cultivars. Telomeric sequences were not detected at the end of the chromosomal fragments corresponding to the location of breaks at 45S sites. Apparently, the transcriptional activity was modified in fragile sites. Variation in the number and size of nucleoli, nucleolar fusions and dissociations were observed. All CMA(+) bands were colocalized with the 45S sites. MenosLolium perenne is considered a high-quality forage widely used in temperate regions to meet the shortage of forage during the winter. In this species, some peculiarities related to cytogenetic aspects have already been described, as the variability in number and position of 45S ribosomal DNA (rDNA) sites and the expression of fragile sites, which require further studies to support the understanding of their causes and consequences. In this way, this study aimed to evaluate the relationship between the expression of fragile sites and functional repetitive sequences (rDNA and telomeric) in chromosomes of diploid and polyploid cultivars of L. perenne. The techniques of FISH, Ag-NOR and fluorescence banding were used to assess the distribution of sites of 45S rDNA, 5S, telomeric sequences, and the transcriptional activity of the 45S ribosomal genes and the distribution of AT- and/or GC-rich sequences in L. perenne, respectively. There was variability in the number and location of 45S rDNA sites, which was not observed for 5S rDNA sites. One of the genotypes showed two 45S rDNA sites on the same chromosome, located in different chromosome arms. Breaks and gaps were found in 45S rDNA sites in most metaphases evaluated for both cultivars. Telomeric sequences were not detected at the end of the chromosomal fragments corresponding to the location of breaks at 45S sites. Apparently, the transcriptional activity was modified in fragile sites. Variation in the number and size of nucleol... Mostrar Tudo |
|
Palavras-Chave: |
45S rDNA; Chromosomal rearrangements; CMA banding; Fragile sites; Ryegrass; Synteny. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
null
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 14 | |
| 6. |  | FRANZIN, A.; MARUYAMA, S.; SINGH, M.; MORE, D.; FERREIRA, B.; RIBEIRO, J.; BISHOP, R.; SANTOS, I. K. F. de M. A systems biology approach for developing an anti-tick vaccine for cattle. In: INTERNATIONAL VETERINARY VACCINES AND DIAGNOSTICS CONFERENCE, 5, 2009, Madison, WI USA. [Proceedings...]. Madison: [s.n.], 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| 7. | | CARVALHO, W. A.; BECHARA, G. H.; MORÉ, D. D.; FERREIRA, B. R.; SILVA, J. S. da; SANTOS, I. K. F. de M. S. Rhipicephalus (Boophilus) microplus: distinct acute phase proteins vary during infestations according to the genetic composition of the bovine hosts, Bos taurus and Bos indicus. Experimental Parasitology, v. 118, p. 587-591, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 8. | | MARUYAMA, S. R.; HORACKOVA, J.; TEIXEIRA, F. R.; MORE, D. D.; GARCIA, G. R.; FERREIRA, B. R.; SANTOS, I. K. F. de M. Reverse immunogenomics for discovery of new antigens for a vaccine against the cattle tick, Rhipicephalus microplus. In: EUROPEAN VETERINARY IMMUNOLOGY WORKSHOP, 3, 2009, Berlin. Abstract Book of ECI. [s. l.]: European Veterinary Immunology Group, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 9. | | SANTOS, I. K. F. DE M.; COSTA, C. H. N.; WIENER, D.; HARN, D. A.; EZEKOWITZ, R. A. B.; EPSTEIN, J. The role of mannose-binding lectin in the resistance to visceral leishmaniasis and in the effector and costimulatory function of macrophages. In: ANNUAL MEETING. NIAID INTERNATIONAL CENTERS FOR TROPICAL DISEASE RESEARCH, 6.,1997, Bethesda, JOINT CONFERENCE OF THE U.S.-JAPAN PARASITIC DISEASES PANELS, 32., 1997, Bethesda. Abstracts...[S.l.]: National Institute of Allergy and Infections,1997. Nao paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 10. | | ANDRADE, T. E. G.; PEÑA, M. S.; FIOROTTI, J.; BIN, R. de S.; CAETANO, A. R.; CONNELLEY, T.; SANTOS, I. K. F. de M. The DRB3 gene of the bovine major histocompatibility complex: discovery, diversity and distribution of alleles in commercial breeds of cattle and applications for development of vaccines. Journal of Dairy Science, v. 107, n. 12, p. 11324–11341, 2024| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 11. | | ABATEPAULO, A. R. R.; CAETANO, A. R.; SANTOS, I. K. F. de M.; MORÉ, D. D.; CARVALHO, W. A.; SILVA, J. S. da. Detection of single nucleotide polymorphisms (SNPs) in bovine immune-response candidate genes for mediating resistance to infestations with the cattle tick, Rhipicephalus (Boophilus) microplus. In: INTERNATIONAL CONFERENCE ON ANIMAL GENETICS, 30., 2006, Porto Seguro, BA. [Proceedings...]. Belo Horizonte, MG: CBRA, 2006. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 12. | | KASHINO, S. S.; RESENDE, J.; SACCO, A. M. S.; ROCHA, C.; PROENÇA, L.; CARVALHO, W. A.; FIRMINO, A. A.; QUEROZ, R.; BENAVIDES, M. V.; GERSHWIN, L. J.; SANTOS, I. K. F. de M. Boophilus microplus: the pattern of bovine immunoglobulin isotype responses to high and low tick infestations. Experimental Parasitology, v. 110, n. 1, p. 12-21, May 2005.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Pecuária Sul. |
| |
| 13. | | ALONSO, D. P.; FERREIRA, A. F. B.; RIBOLLA, P. E. M.; SANTOS, I. K. F. de M.; CRUZ, M. do S. P. e; CARVALHO, F. A. de; ABATEPAULO, A. R. R.; COSTA, D. L.; WERNECK, G. L.; FARIAS, T. J. C.; SOARES, M. J. S.; COSTA, C. H. N. Genotypes of the mannan-binding lectin gene and susceptibility to visceral leishmaniasis and clinical complications. The Journal of Infectious Diseases, v. 195, p. 1212-1217, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 14. | | CARVALHO, W. A.; IANELLA, P.; ARNOLDI, F. G. C.; CAETANO, A. R.; MARUYAMA, S. R.; FERREIRA, B. R.; CONTI, L. H. A.; SILVA, M. R. M. da; PAULA, J. O. F.; MAIA, A. A. M.; SANTOS, I. K. F. de M. Haplotypes of the bovine IgG2 heavy gamma chain in tick-resistant and tick-susceptible breeds of cattle. Immunogenetics, v. 63, p. 319-324, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 14 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
