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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/05/2014 |
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Data da última atualização: |
24/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALICENTE, F. H.; SILVA, R. B. da. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; ROSANE BEZERRA DA SILVA, BOLSISTA. |
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Título: |
fAFLP analysis of Brazilian Bacillus thuringiensis isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
SpringerPlus, v. 3, n. 256, 2014. |
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DOI: |
10.1186/2193-1801-3-256 |
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Idioma: |
Inglês |
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Conteúdo: |
A total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest subspecies to this group was B. thuringiensis subsp thuringiensis (HD2) with 52% of similarity. This similarity may be due to the fact that these organism exchange genetic material by conjugation, and it is relatively common to have evolutionary characteristics of their ancestors. MenosA total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest s... Mostrar Tudo |
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Palavras-Chave: |
Bacillus thruringiensis; Diversidade genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02280naa a2200169 a 4500
001 1987279
005 2017-05-24
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/2193-1801-3-256$2DOI
100 1 $aVALICENTE, F. H.
245 $afAFLP analysis of Brazilian Bacillus thuringiensis isolates.$h[electronic resource]
260 $c2014
520 $aA total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest subspecies to this group was B. thuringiensis subsp thuringiensis (HD2) with 52% of similarity. This similarity may be due to the fact that these organism exchange genetic material by conjugation, and it is relatively common to have evolutionary characteristics of their ancestors.
653 $aBacillus thruringiensis
653 $aDiversidade genética
700 1 $aSILVA, R. B. da
773 $tSpringerPlus$gv. 3, n. 256, 2014.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 10 | |
| 1. |  | SANTOS, C. X. dos; SILVA, L. R. de L.; NASCIMENTO, W. M. O. do. Avaliação da precocidade da produção em clones de camucamuzeiro. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 26., 2023, Belém, PA. Anais [...]. Belém, PA: Embrapa Amazônia Oriental, 2024. p. 47-48. (Embrapa Amazônia Oriental. Eventos técnicos & científicos, 2).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | SILVA, L. R. de L.; NASCIMENTO, W. M. O. do; FRANCO, E. C.; SANTOS, C. X. dos. Produção e biometria em frutos de clones de Myrciaria dubia. In: SEMINÁRIO INTERNACIONAL DE DESENVOLVIMENTO RURAL SUSTENTÁVEL COOPERATIVISMO E ECONOMIA SOLIDÁRIA, 17.; FEIRA DE CIÊNCIA, TECNOLOGIA E INOVAÇÃO SOCIAL, 8., 2024, Castanhal. [Anais...]. Castanhal, 2024. 10 p. XVII SICOOPES.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | SANTOS, C. X. dos; VARGAS, D. P.; MASIERO, D. de S.; DUTRA, L. F.; SILVA, S. D. dos A. e. Enraizamento e aclimatização de diferentes variedades de cana-de açúcar. In: SIMPÓSIO ESTADUAL DE AGROENERGIA; REUNIÃO TÉCNICA DE AGROENERGIA - RS, 5.; ENCONTRO DE ENERGIAS RENOVÁVEIS NA AGRICULTURA FAMILIAR, 2., Pelotas, 2014. Anais... Pelotas: Embrapa Clima Temperado, 2014. 1 pen card.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | MASIERO, D. S.; VARGAS, D. P.; SANTOS, C. X. dos; FERREIRA, L. V.; FORMOSA, R. S.; DUTRA, L. F. Encapsulamento e desidratação de gemas laterais de framboeseira CV autumn bliss. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | FRANCO, E. C.; NASCIMENTO, W. M. O. do; SANTOS, C. X. dos; SILVA, L. R. de L. Substratos para o crescimento de mudas em genótipos de camucamuzeiro. In: SEMINÁRIO INTERNACIONAL DE DESENVOLVIMENTO RURAL SUSTENTÁVEL COOPERATIVISMO E ECONOMIA SOLIDÁRIA, 17.; FEIRA DE CIÊNCIA, TECNOLOGIA E INOVAÇÃO SOCIAL, 8., 2024, Castanhal. [Anais...]. Castanhal, 2024. 11 p. XVII SICOOPES.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | FERREIRA, L. V.; VARGAS, D. P.; MASIEIRO, D. de S.; SANTOS, C. X. dos; FORMOSO, R. S.; DUTRA, L. F. Conservação in vitro de morangueiro benícia via crescimento lento. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | SILVA, L. R. de L.; NASCIMENTO, W. M. O. do; SANTOS, C. X. dos; FRANCO, E. C. Morfometria de flores, folhas e frutos em clones de castanheira-do-brasil. In: SEMINÁRIO INTERNACIONAL DE DESENVOLVIMENTO RURAL SUSTENTÁVEL, COOPERATIVISMO E ECONOMIA SOLIDÁRIA, 18., 2025, Castanhal. Anais [...]. Castanhal: IFPA, 2025. XVIII SICOOPES.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | COSTA, R. R. da; VARGAS, D. P.; SANTOS, C. X. dos; MASIERO, D. de S.; DUTRA, L. F.; ANTUNES, L. E. C. Multiplicação in vitro de mirtileiro seleção blue 41. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 10 | |
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