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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/12/2013 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
D'ALESSANDRO, C. P.; JONES, L. R.; HUMBER, R. A.; LÓPEZ LASTRA, C. C.; SOSA-GOMEZ, D. R. |
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Afiliação: |
CELESTE P. D'ALESSANDRO, CEPAVE/CONICET-UNLP; LEANDRO R. JONES, CONICET; RICHARD A. HUMBER, USDA-ARS; CLAUDIA C. LÓPEZ LASTRA, CEPAVE/CONICET-UNLP; DANIEL RICARDO SOSA-GOMEZ, CNPSO. |
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Título: |
Characterization and phylogeny of Isaria spp. strains (Ascomycota: Hypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-alpha sequences. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Basic Microbiology, Weinheim, v. 53, n. 12, p. 1-11, Nov. 2013. |
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DOI: |
10.1002/jobm.201300499 |
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Idioma: |
Inglês |
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Conteúdo: |
The elongation factor 1-alpha (EF1-α) and the internal transcribed spacer (ITS) regions ITS1 and ITS2 (ITS1-5.8S-ITS2) sequences were used to characterize and to identify Isaria isolates from Argentina, Mexico, and Brazil, as well as to study the phylogenetic relationships among these isolates and other related fungi from the order Hypocreales. The molecular characterization, which was performed by PCR-RFLP of EF1-α and ITS1-5.8-ITS2 genes, was useful for resolving representative isolates of Isaria fumosorosea, Isaria farinosa, and Isaria tenuipes and to confirm the taxonomic identity of fungi from Argentina, Mexico, and Brazil. The phylogenetic analyses showed three clades corresponding to three families of Hypocreales. The genus Isaria was confirmed as polyphyletic and in family Cordycipitaceae, Isaria species were related to anamorphic species of Beauveria, Lecanicillium, and Simplicillium and to teleomorphic Cordyceps and Torrubiella. Therefore, EF1-α and ITS1-5.8S-ITS2 genes were found to be powerful tools for improving the characterization, identification, and phylogenetic relationship of the Isaria species and other entomopathogenic fungi. |
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Thesagro: |
Filogenia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01847naa a2200193 a 4500
001 1973450
005 2017-08-03
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1002/jobm.201300499$2DOI
100 1 $aD'ALESSANDRO, C. P.
245 $aCharacterization and phylogeny of Isaria spp. strains (Ascomycota$bHypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-alpha sequences.$h[electronic resource]
260 $c2013
520 $aThe elongation factor 1-alpha (EF1-α) and the internal transcribed spacer (ITS) regions ITS1 and ITS2 (ITS1-5.8S-ITS2) sequences were used to characterize and to identify Isaria isolates from Argentina, Mexico, and Brazil, as well as to study the phylogenetic relationships among these isolates and other related fungi from the order Hypocreales. The molecular characterization, which was performed by PCR-RFLP of EF1-α and ITS1-5.8-ITS2 genes, was useful for resolving representative isolates of Isaria fumosorosea, Isaria farinosa, and Isaria tenuipes and to confirm the taxonomic identity of fungi from Argentina, Mexico, and Brazil. The phylogenetic analyses showed three clades corresponding to three families of Hypocreales. The genus Isaria was confirmed as polyphyletic and in family Cordycipitaceae, Isaria species were related to anamorphic species of Beauveria, Lecanicillium, and Simplicillium and to teleomorphic Cordyceps and Torrubiella. Therefore, EF1-α and ITS1-5.8S-ITS2 genes were found to be powerful tools for improving the characterization, identification, and phylogenetic relationship of the Isaria species and other entomopathogenic fungi.
650 $aFilogenia
700 1 $aJONES, L. R.
700 1 $aHUMBER, R. A.
700 1 $aLÓPEZ LASTRA, C. C.
700 1 $aSOSA-GOMEZ, D. R.
773 $tJournal of Basic Microbiology, Weinheim$gv. 53, n. 12, p. 1-11, Nov. 2013.
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| 1. |  | SAITO, M. L. Avaliacao de risco versus boas praticas laboratoriais. In: DE NARDO; E.A.B.; CAPALBO, D.M.F.; OLIVEIRA, M.C.B.; MORAES, G.J. de., eds. Analise de risco e avaliacao do impacto ambiental decorrente do uso de agentes de controle biologico: memoria do workshop. Jaguariuna: EMBRAPA-CNPMA, 1995. p.51-53 (EMBRAPA-CNPMA. Documentos).| Biblioteca(s): Embrapa Meio Ambiente. |
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