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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/10/1994 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAGÃO, F. J. L.; GROSSI DE SA, M. F.; DAVEY, M. R.; BRASILEIRO, A. C. M.; FARIA, J. C.; RECH, E. L. |
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Afiliação: |
FRANCISCO JOSE LIMA ARAGAO, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; M. R. DAVEY, UNIVERSITY OF NOTTINGHAM; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; JOSIAS CORREA DE FARIA, CNPAF; ELIBIO LEOPOLDO RECH FILHO, CENARGEN. |
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Título: |
Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 12, n. 9, p. 483-490, July 1993. |
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DOI: |
https://doi.org/10.1007/BF00236092 |
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Idioma: |
Inglês |
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Conteúdo: |
The parameters influencing transient expression of the beta-glucuronidase gene in bean embryonic axes, cotyledons, apical meristems and leaves were evaluated after gene delivery with an electrical particle acceleration device. A calcium-spermidine procedure for coating gold particles with DNA resulted in higher levels of GUS expression with lower concentrations of gold particles compared with a calcium phosphate procedure. The DNA concentration, distance between the discharge chamber and the retaining screen and the vacuum in the apparatus also influenced gene delivery. Sections prepared for light and electron microscopy showed the localisation, within target cells, of gold particles used to deliver the DNA. Immunolocalization of foreign gene expression within cells confirmed an even distribution of gene product throughout the cell cytoplasm. |
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Palavras-Chave: |
Aceleracao; Bean; Expression; Particle; Particula; Transient. |
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Thesagro: |
Feijão; Gene; Phaseolus Vulgaris. |
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Thesaurus Nal: |
acceleration. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01763naa a2200313 a 4500
001 1197634
005 2022-05-09
008 1993 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/BF00236092$2DOI
100 1 $aARAGÃO, F. J. L.
245 $aFactors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device.$h[electronic resource]
260 $c1993
520 $aThe parameters influencing transient expression of the beta-glucuronidase gene in bean embryonic axes, cotyledons, apical meristems and leaves were evaluated after gene delivery with an electrical particle acceleration device. A calcium-spermidine procedure for coating gold particles with DNA resulted in higher levels of GUS expression with lower concentrations of gold particles compared with a calcium phosphate procedure. The DNA concentration, distance between the discharge chamber and the retaining screen and the vacuum in the apparatus also influenced gene delivery. Sections prepared for light and electron microscopy showed the localisation, within target cells, of gold particles used to deliver the DNA. Immunolocalization of foreign gene expression within cells confirmed an even distribution of gene product throughout the cell cytoplasm.
650 $aacceleration
650 $aFeijão
650 $aGene
650 $aPhaseolus Vulgaris
653 $aAceleracao
653 $aBean
653 $aExpression
653 $aParticle
653 $aParticula
653 $aTransient
700 1 $aGROSSI DE SA, M. F.
700 1 $aDAVEY, M. R.
700 1 $aBRASILEIRO, A. C. M.
700 1 $aFARIA, J. C.
700 1 $aRECH, E. L.
773 $tPlant Cell Reports$gv. 12, n. 9, p. 483-490, July 1993.
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