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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
16/07/1991 |
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Data da última atualização: |
27/01/2025 |
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Autoria: |
THOMAS, B. D.; CLARKSON, D. T.; BRAIN, P. |
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Título: |
Kinetics of phosphorus uptake by the germ-tubes of the vesicular arbuscular mycorrhizal fungus Gigaspora-margarita. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
New Phytologist, v. 116, n. 4, p. 647-654, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
We characterized kinetically the phosphorus (P) transport systems present in the germ-tubes of the vesicular-arbuscular (VA) mycorrhizal fungus, Gigaspora margarita Becker & Hall. Germinated spores of G. margarita were grown without P or with 1000 μM P for 10 d. The rate of P uptake by these spores and their associated germ-tubes was measured at external P concentrations ranging from 1 to 10000 μM and in the presence or absence of an uncoupler of oxidative phosphorylation. Two transport systems for P were identified in the germ-tubes of G. margarita grown without P. System I, an active high affinity system with a Km of 1.8–3.1 μM and a Vmax of 3.1–3.6 nmol mg protein−1h−1and System II, a passive low affinity system with a Km of 10200–11 300 μM and a Vmax of 445–895 nmol mg protein−1h−1. These two systems operated simultaneously at concentrations of P ranging from 1 to 10000 μM. Two transport systems for P were also identified in the germ-tubes of G. margarita grown with 1000 μM P. The Km of System I in these germ-tubes (13.4 μM) was increased relative to the Km of System I in germ-tubes grown without P. There was also evidence to suggest that the Km of System II was increased in germ-tubes grown with 1000 μM P. The values of Km and Vmax for the P transport systems in the germ-tubes of G. margarita are similar to those observed in the hyphae of other fungi and in plant roots. |
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Thesagro: |
Fósforo; Micorriza Vesicular Arbuscular. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01961naa a2200169 a 4500
001 1279584
005 2025-01-27
008 1990 bl uuuu u00u1 u #d
100 1 $aTHOMAS, B. D.
245 $aKinetics of phosphorus uptake by the germ-tubes of the vesicular arbuscular mycorrhizal fungus Gigaspora-margarita.$h[electronic resource]
260 $c1990
520 $aWe characterized kinetically the phosphorus (P) transport systems present in the germ-tubes of the vesicular-arbuscular (VA) mycorrhizal fungus, Gigaspora margarita Becker & Hall. Germinated spores of G. margarita were grown without P or with 1000 μM P for 10 d. The rate of P uptake by these spores and their associated germ-tubes was measured at external P concentrations ranging from 1 to 10000 μM and in the presence or absence of an uncoupler of oxidative phosphorylation. Two transport systems for P were identified in the germ-tubes of G. margarita grown without P. System I, an active high affinity system with a Km of 1.8–3.1 μM and a Vmax of 3.1–3.6 nmol mg protein−1h−1and System II, a passive low affinity system with a Km of 10200–11 300 μM and a Vmax of 445–895 nmol mg protein−1h−1. These two systems operated simultaneously at concentrations of P ranging from 1 to 10000 μM. Two transport systems for P were also identified in the germ-tubes of G. margarita grown with 1000 μM P. The Km of System I in these germ-tubes (13.4 μM) was increased relative to the Km of System I in germ-tubes grown without P. There was also evidence to suggest that the Km of System II was increased in germ-tubes grown with 1000 μM P. The values of Km and Vmax for the P transport systems in the germ-tubes of G. margarita are similar to those observed in the hyphae of other fungi and in plant roots.
650 $aFósforo
650 $aMicorriza Vesicular Arbuscular
700 1 $aCLARKSON, D. T.
700 1 $aBRAIN, P.
773 $tNew Phytologist$gv. 116, n. 4, p. 647-654, 1990.
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| 1. |  | CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N. de; LAGE, A. P.; BLAGITZ, M. G.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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| Registros recuperados : 3 | |
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