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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
08/02/2019 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SILVA, M. R.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; BARTLEWSKI, P. M.; SOUZA-FABJAN, J. M. G. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; FABIANA NUNES ZAMBRINI, UNIVERSIDADE FEDERAL DE VIÇOSA; JOSÉ DOMINGOS GUIMARÃES, UNIVERSIDADE FEDERAL DE VIÇOSA; MARCIO ROBERTO SILVA, CNPGL; MARIA EMILIA FRANCO OLIVEIRA, UNIVERSIDADE ESTADUAL PAULISTA; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE; PAWEL MIECZYSLAW BARTLEWSKI, UNIVERSITY OF GUELPH; JOANNA MARIA GONÇALVES SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Combined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 1, p. 118-125, Sept. 2019. |
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DOI: |
http://dx.doi.org/10.1111/rda.13318 |
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Idioma: |
Inglês |
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Conteúdo: |
Contents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route. MenosContents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., tr... Mostrar Tudo |
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Palavras-Chave: |
Cervical dilation; Drugs effects; Oestradiol benzoate; Synchronized females; Transcervical embryo collection. |
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Thesagro: |
Estradiol; Hormônio; Ovino; Reprodução Animal; Transferência de Embrião. |
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Thesaurus Nal: |
Cloprostenol; Oxytocin; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02929naa a2200373 a 4500
001 2105726
005 2025-01-10
008 2019 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1111/rda.13318$2DOI
100 1 $aFONSECA, J. F. da
245 $aCombined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes.$h[electronic resource]
260 $c2019
520 $aContents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.
650 $aCloprostenol
650 $aOxytocin
650 $aSheep
650 $aEstradiol
650 $aHormônio
650 $aOvino
650 $aReprodução Animal
650 $aTransferência de Embrião
653 $aCervical dilation
653 $aDrugs effects
653 $aOestradiol benzoate
653 $aSynchronized females
653 $aTranscervical embryo collection
700 1 $aZAMBRINI, F. N.
700 1 $aGUIMARÃES, J. D.
700 1 $aSILVA, M. R.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aBRANDÃO, F. Z.
700 1 $aBARTLEWSKI, P. M.
700 1 $aSOUZA-FABJAN, J. M. G.
773 $tReproduction in Domestic Animals$gv. 54, n. 1, p. 118-125, Sept. 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 5 | |
| 1. |  | RODRIGUES, M. J. C.; SAGRILO, E.; OLIVEIRA JUNIOR, J. O. L. de; SOUSA, D. C. de; LIMA, M. G. M.; SOUZA, H. A. de. Alterações do pH e floculação de argila em solo submetido à calagem na fronteira agrícola de Paragominas, Pará. In: ENCONTRO DE CIÊNCIA DO SOLO DA AMAZÔNIA ORIENTAL, 4., 2024, Palmas. Desafios para a produção agrícola: Anais. Palmas: SBCS: UNITINS: UFMA, 2025. Amazon Soil 2024.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 2. | | SOUZA, H. A. de; SAGRILO, E.; OLIVEIRA JUNIOR, J. O. L. de; SOUSA, D. C. de; GARANHANI, C. A. C.; PONTES, S. F.; ANDRADE, H. A. de F.; COSTA, P. M.; ALMEIDA, H. J. de; RODRIGUES, M. J. C. Acúmulo e exportação de macro e de micronutrientes em grãos de gergelim cultivado sob doses de calcário e gesso em Paragominas, Pará. Teresina: Embrapa Meio-Norte, 2025. 10 p. (Embrapa Meio-Norte. Boletim de Pesquisa e Desenvolvimento, 164). ODS-2.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 3. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 4. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALUU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPOSITO, M. B.; ARAUJO, W. L. de; PIZZIRANI-KLEINER, A. A. Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Fungal Biology, v. 117, n. 7-8, p. 463-576, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. S.; FERREIRA, A.; SEBASTIANES, F. L. S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L.; BOGAS, A. C.; PIZZIRANI-KLEINER, A. A. Guignardia citricarpa: Agrobacterium-mediated transformation and functional GFP expression. In: CONGRESS OF EUROPEAN MICROBIOLOGISTS - FEMS, 4., 2011, Geneva, Switzerland. [s.L.]: Kenes Group, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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