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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
24/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. |
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Afiliação: |
JUACI VITORIA MALAQUIAS, CPAC; UNIVERSIDADE DE BRASILIA; CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cryobiology, v. 70, p. 90-94, 2015. |
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ISSN: |
0011-2240 |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2015.01.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique. MenosAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cry... Mostrar Tudo |
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Thesagro: |
Congelamento; Conservação; Criopreservação; Esperma; Inseminação artificial; Suino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02370naa a2200277 a 4500
001 2024328
005 2025-04-24
008 2015 bl uuuu u00u1 u #d
022 $a0011-2240
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2015.01.001$2DOI
100 1 $aSILVA, C. G.
245 $aCryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.$h[electronic resource]
260 $c2015
520 $aAbstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
650 $aCongelamento
650 $aConservação
650 $aCriopreservação
650 $aEsperma
650 $aInseminação artificial
650 $aSuino
700 1 $aCUNHA, E. R.
700 1 $aBLUME, G. R.
700 1 $aMALAQUIAS, J. V.
700 1 $aBÁO, S. N.
700 1 $aMARTINS, C. F.
773 $tCryobiology$gv. 70, p. 90-94, 2015.
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| 7. |  | BELTRAMIN, F. S.; RODRIGUES, A. P.; BARBOSA, L. R. Inovações para o controle biológico de Thaumastocoris peregrinus (Hemiptera, Thaumastocoridae) pelo parasitóide oófago Cleruchoides noackae (Hymenoptera, Mymaridae). In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 12., 2013, Colombo. Anais. Colombo: Embrapa Florestas, 2013. (Embrapa Florestas. Documentos, 253). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 9. | | MAIA, J. N.; BARBOSA, L. R.; RODRIGUES, A. P. Efeito do número de gerações de Cleruchoides noackae (Hymenoptera, Mymaridae) no parasitismo de Thaumastocoris peregrinus (Hemiptera, Thaumastocoridae). In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 14., 2015, Colombo. Anais... Colombo: Embrapa Florestas, 2015. p. 52-53. (Embrapa Florestas. Documentos, 278). Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 15. | | BRAUNER, P. A.; RODRIGUES, A. P. D. C.; LAURA, V. A.; FREITAS, M. E. de. Absorção de água por sementes de soja no método de imersão em água destilada. In: SEMINÁRIO INTERNO DE INICIAÇÃO CIENTÍFICA DA UNIDERP, 2., 2007, Campo Grande, MS. Anais... Campo Grande, MS: Ed. da UNIDERP, 2007. 3 p. 1 CD-ROM. CNPGC.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 18. | | MORO, G. V.; RODRIGUES, A. P. O.; TORATI, L. S.; BARROSO, R. M.; LUNDSTEDT, L. M. Anatomia e fisiologia de peixes de água doce. In: RODRIGUES, A. P. O.; LIMA, A. F.; ALVES, A. L.; ROSA, D. K.; TORATI, L. S.; SANTOS, V. R. V. dos (Ed.). Piscicultura de água doce: multiplicando conhecimentos. Brasília, DF: Embrapa, 2013. p. 71-95. il.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 19. | | RODRIGUES, A. P.; BECCHI, L. K.; WILCKEN, C. F.; FOERSTER, L. A.; BARBOSA, L. R. Armazenamento de ovos de Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae) parasitados por Cleruchoides noackae (Hymenoptera: Mymaridae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., 2016, Maceió. Anais... Brasília, DF : Embrapa, 2016. p. 470.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 181 | |
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