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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
05/11/2024 |
Data da última atualização: |
05/11/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, L. P. da; SILVA, M. de O.; BORGES, M. S.; PAGOTO, J. M.; WATANABE, Y. F.; OLIVEIRA, C. S.; MERCADANTE, M. E. Z.; MONTEIRO, F. M. |
Afiliação: |
LETÍCIA PADOVANI DA SILVA, CENTRO AVANÇADO DE PESQUISA E DESENVOLVIMENTO DE BOVINOS DE CORTE; MARINA DE OLIVEIRA SILVA, UNIVERSIDADE ESTADUAL PAULISTA; MARCELO SANT’ANA BORGES, UNIVERSIDADE ESTADUAL PAULISTA; JAINE MARTELO PAGOTO, UNIVERSIDADE ESTADUAL PAULISTA; YEDA FUMIE WATANABE, WTA SOLUÇÕES PARA REPRODUÇÃO ANIMAL; CLARA SLADE OLIVEIRA, CNPGL; MARIA EUGÊNIA ZERLOTTI MERCADANTE, CENTRO AVANÇADO DE PESQUISA E DESENVOLVIMENTO DE BOVINOS DE CORTE; FABIO MORATO MONTEIRO, UNIVERSIDADE ESTADUAL PAULISTA. |
Título: |
Injectable progesterone in 13-month-old prepubertal Nellore heifers: effects on in vitro embryo production and pregnancy rate. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Tropical Animal Health and Production, v. 56, n. 8, 357, 2024. |
DOI: |
https://doi.org/10.1007/s11250-024-04205-6 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to evaluate the effect of injectable progesterone administration before ovum pick-up (OPU) on in vitro embryo production (IVEP) and pregnancy rate in prepubertal Nellore heifers. Twenty-three Nellore females, 13.04±0.8 months of age, were randomly assigned to two groups: control group (no progesterone application) and progesterone group (intramuscular administration of 1 mL [150 mg/mL] progesterone 7 days before OPU). The second OPU was performed after 28 days (crossover between groups). After OPU, oocytes were selected for IVEP and a sample was fixed in 4% paraformaldehyde. Viable oocytes were subjected to immunofluorescence staining for IGFBP2 and caspase-3. For each oocyte, one image was generated per channel (A555 and A488 filters) and analyzed with the ImageJ program for the quantification of fluorescence intensity (in pixels). The produced embryos were transferred to multiparous Nellore cows and pregnancy was diagnosed by ultrasound 30 days after transfer. Data were analyzed using the MIXED and GLIMMIX procedure of SAS®. Treatment with injectable progesterone had no effect on the number or size of the antral follicles of heifers. No differences between the control and progesterone groups were observed for total number of aspirated oocytes (P=0.78), viable oocytes (P=0.80), cleavage rate (P=0.60), or blastocyst rate (P=0.56). There were no differences in pixel intensity between the two groups, nor in the levels of IGFBP2 or caspase-3. However, a difference was observed in the pregnancy rate of embryos produced from heifers of the progesterone group (42.1%; 32/76) when compared to the rate of embryos produced from control heifers (28.4%; 23/81) (P=0.02). The use of injectable progesterone before OPU in prepubertal Nellore heifers does not improve IVEP but increases the pregnancy rate after transfer. MenosThe aim of this study was to evaluate the effect of injectable progesterone administration before ovum pick-up (OPU) on in vitro embryo production (IVEP) and pregnancy rate in prepubertal Nellore heifers. Twenty-three Nellore females, 13.04±0.8 months of age, were randomly assigned to two groups: control group (no progesterone application) and progesterone group (intramuscular administration of 1 mL [150 mg/mL] progesterone 7 days before OPU). The second OPU was performed after 28 days (crossover between groups). After OPU, oocytes were selected for IVEP and a sample was fixed in 4% paraformaldehyde. Viable oocytes were subjected to immunofluorescence staining for IGFBP2 and caspase-3. For each oocyte, one image was generated per channel (A555 and A488 filters) and analyzed with the ImageJ program for the quantification of fluorescence intensity (in pixels). The produced embryos were transferred to multiparous Nellore cows and pregnancy was diagnosed by ultrasound 30 days after transfer. Data were analyzed using the MIXED and GLIMMIX procedure of SAS®. Treatment with injectable progesterone had no effect on the number or size of the antral follicles of heifers. No differences between the control and progesterone groups were observed for total number of aspirated oocytes (P=0.78), viable oocytes (P=0.80), cleavage rate (P=0.60), or blastocyst rate (P=0.56). There were no differences in pixel intensity between the two groups, nor in the levels of IGFBP2 or caspase-3. However, ... Mostrar Tudo |
Palavras-Chave: |
Aspiração folicular. |
Thesagro: |
Bovino; Embrião Animal; Progesterona; Reprodução Animal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02746naa a2200277 a 4500 001 2168827 005 2024-11-05 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s11250-024-04205-6$2DOI 100 1 $aSILVA, L. P. da 245 $aInjectable progesterone in 13-month-old prepubertal Nellore heifers$beffects on in vitro embryo production and pregnancy rate.$h[electronic resource] 260 $c2024 520 $aThe aim of this study was to evaluate the effect of injectable progesterone administration before ovum pick-up (OPU) on in vitro embryo production (IVEP) and pregnancy rate in prepubertal Nellore heifers. Twenty-three Nellore females, 13.04±0.8 months of age, were randomly assigned to two groups: control group (no progesterone application) and progesterone group (intramuscular administration of 1 mL [150 mg/mL] progesterone 7 days before OPU). The second OPU was performed after 28 days (crossover between groups). After OPU, oocytes were selected for IVEP and a sample was fixed in 4% paraformaldehyde. Viable oocytes were subjected to immunofluorescence staining for IGFBP2 and caspase-3. For each oocyte, one image was generated per channel (A555 and A488 filters) and analyzed with the ImageJ program for the quantification of fluorescence intensity (in pixels). The produced embryos were transferred to multiparous Nellore cows and pregnancy was diagnosed by ultrasound 30 days after transfer. Data were analyzed using the MIXED and GLIMMIX procedure of SAS®. Treatment with injectable progesterone had no effect on the number or size of the antral follicles of heifers. No differences between the control and progesterone groups were observed for total number of aspirated oocytes (P=0.78), viable oocytes (P=0.80), cleavage rate (P=0.60), or blastocyst rate (P=0.56). There were no differences in pixel intensity between the two groups, nor in the levels of IGFBP2 or caspase-3. However, a difference was observed in the pregnancy rate of embryos produced from heifers of the progesterone group (42.1%; 32/76) when compared to the rate of embryos produced from control heifers (28.4%; 23/81) (P=0.02). The use of injectable progesterone before OPU in prepubertal Nellore heifers does not improve IVEP but increases the pregnancy rate after transfer. 650 $aBovino 650 $aEmbrião Animal 650 $aProgesterona 650 $aReprodução Animal 653 $aAspiração folicular 700 1 $aSILVA, M. de O. 700 1 $aBORGES, M. S. 700 1 $aPAGOTO, J. M. 700 1 $aWATANABE, Y. F. 700 1 $aOLIVEIRA, C. S. 700 1 $aMERCADANTE, M. E. Z. 700 1 $aMONTEIRO, F. M. 773 $tTropical Animal Health and Production$gv. 56, n. 8, 357, 2024.
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1. |  | COSTA, L. C.; WONDRACEK, D. C.; ROCHA, R. A.; RAMOS, D. M.; VALLS, J. F. M. Stigma tip morphology of Arachis (Fabaceae) species from seven taxonomic sections, with emphasis on sections Extranervosae and Heteranthae. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 59Tipo: Resumo em Anais de Congresso |
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