| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
19/03/2010 |
|
Data da última atualização: |
28/05/2025 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. |
|
Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; FRANCISMAR CORREA MARCELINO, CNPSO; FERNANDO GOMES BARCELLOS; ELISETE PAINS RODRIGUES; MANUEL MEGIAS, UNIVERSIDAD DE SEVILHA; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
|
Título: |
The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Functional & Integrative Genomics, v. 10, n. 3, p. 425-431, Aug. 2010. |
|
DOI: |
10.1007/s10142-009-0151-x |
|
Idioma: |
Inglês |
|
Conteúdo: |
Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed. |
|
Palavras-Chave: |
Reverse transcription quantitative-PCR. |
|
Thesagro: |
Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose. |
|
Thesaurus Nal: |
Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02216naa a2200325 a 4500
001 1661862
005 2025-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s10142-009-0151-x$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aThe nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.$h[electronic resource]
260 $c2010
520 $aRhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.
650 $aBeans
650 $aGenes
650 $aNitrogen fixation
650 $aRhizobium tropici
650 $aSymbiosis
650 $aFeijao
650 $aFixacao de nitrogenio
650 $aGene
650 $aPhaseolus Vulgaris
650 $aSimbiose
653 $aReverse transcription quantitative-PCR
700 1 $aMARCELINO, F. C.
700 1 $aBARCELLOS, F. G.
700 1 $aRODRIGUES, E. P.
700 1 $aMEGIAS, M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 10, n. 3, p. 425-431, Aug. 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 3 | |
| 1. |  | CALLAHAM JÚNIOR, M. A.; SNYDER, B. A.; HENDRIX, P. F.; RICHTER, D. de B. Exotic earthworms as friend and foe: Are they indicators of soil quality and health, or of soil damage and disturbance? In: ENCONTRO LATINO-AMERICANO DE ECOLOGIA E TAXONOMIA DE OLIGOQUETAS, 4., 2010, Curitiba. Minhocas como bioindicadoras ambientais: princípios e práticas: anais. Colombo: Embrapa Florestas, 2010. 1 CD-ROM. (Embrapa Florestas. Documentos, 199). 1 CD-ROM.| Biblioteca(s): Embrapa Florestas. |
|   |
| 2. | | SMITH, P.; DAVIES, C. A.; OGLE, S.; ZANCHI, G.; BELLARBY, J.; BIRD, N.; BODDEY, R. M.; NcNAMARA, N. P.; POLWSON, D.; COWIE, A.; VAN NOORDWIJK, M.; DAVIS, S. C.; RICHTER, D.; KRYZANOWSKI, L.; VAN WIJK, M.; STUART, J.; KIRTON, A.; EGGAR, D.; NEWTON-CROSS, G.; ADHYA, T.; BRAIMOH, A. K. Towards an integrated global framework to assess the impacts of land use and management change on soil carbon: current capability and future vision. Global Change Biology, v. 18, n. 7, p. 2089-2101, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
|  |
| 3. | | MILNE, E.; BANWART, S. A.; NOELLEMEYER, E.; ABSON, D. J.; BALLABIO, C.; BAMPA, F.; BATIONO, A.; BATJES, N. H.; BERNOUX, M.; BHATTACHARYYA, T.; BLACK, H.; BUSCHIAZZO, D. E.; CAI, Z.; CERRI, C. E.; KUN, C.; COMPAGNONE, C.; CONANT, R.; COUTINHO, H. L. C.; BROGNIEZ, D. de; BALIEIRO, F. de C.; DUFFY, C.; FELLER, C.; FIDALGO, E. C. C.; SILVA, C. F. da; FUNK, R.; GAUDIG, G.; GICHERU, P. T.; GOLDHABER, M.; GOTTSCHALK, P.; GOULET, F.; GOVERSE, T.; GRATHWOHL, P.; JOOSTEN, H.; KAMONI, P. T.; KIHARA, J.; KRAWCZYNSKI, R.; SCALA JUNIOR, N. la; LEMANCEAU, P.; LI, L.; LI, Z.; LUGATO, E.; MARON, P. A.; MARTIUS, C.; MELILLO, J.; MONTANARELLA, L.; NIKOLAIDIS, N.; NZIGUHEBA, G.; PAN, G.; PASCUAL, U.; PAUSTIAN, K.; PIÑEIRO, G.; POWLSON, D.; QUIROGA, A.; RICHTER, D.; SIGWALT, A.; SIX, J.; SMITH, J.; SMITH, P.; STOCKING, M.; TANNEBERGER, F.; TERMANSEN, M.; NOORDWIJK, M. van; WESEMAEL, B. van; VARGAS, R.; VICTORIA, R. L.; WASWA, B.; WERNER, D.; WICHMANN, S.; WICHTMANN, W.; ZHANG, X.; ZHAO, Y.; ZHENG, J.; ZHENG, J. Soil carbon, multiple benefits. Environmental Development, v. 13, p. 33-38, Jan. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Solos. |
| |
| Registros recuperados : 3 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
