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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Meio Ambiente. |
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Data corrente: |
13/09/2022 |
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Data da última atualização: |
14/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOCATELLI, G. O.; PIMENTEL, M. F.; BUENO, L. A.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L. |
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Afiliação: |
GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARIA FERNANDA PIMENTEL, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LUCIANO AVALLONE BUENO, UNIVERSIDADE FEDERAL DO ABC; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
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Título: |
Production of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biocatalysis and Agricultural Biotechnology, v. 44, article 102455, 2022. |
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ISSN: |
1878-8181 |
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DOI: |
https://doi.org/10.1016/j.bcab.2022.102455 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development. |
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Palavras-Chave: |
Autolyzed yeast; Biofungicides; C N ratio; Microsclerotia. |
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Thesagro: |
Carbono; Farinha; Fermentação; Fungo Para Controle Biológico; Nitrogênio; Sorgo; Trichoderma. |
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Thesaurus Nal: |
Biological control agents; Liquid state fermentation; Sorghum flour; Trichoderma asperellum. |
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Categoria do assunto: |
H Saúde e Patologia
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02407naa a2200385 a 4500
001 2146414
005 2022-09-14
008 2022 bl uuuu u00u1 u #d
022 $a1878-8181
024 7 $ahttps://doi.org/10.1016/j.bcab.2022.102455$2DOI
100 1 $aLOCATELLI, G. O.
245 $aProduction of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources.$h[electronic resource]
260 $c2022
520 $aAbstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development.
650 $aBiological control agents
650 $aLiquid state fermentation
650 $aSorghum flour
650 $aTrichoderma asperellum
650 $aCarbono
650 $aFarinha
650 $aFermentação
650 $aFungo Para Controle Biológico
650 $aNitrogênio
650 $aSorgo
650 $aTrichoderma
653 $aAutolyzed yeast
653 $aBiofungicides
653 $aC N ratio
653 $aMicrosclerotia
700 1 $aPIMENTEL, M. F.
700 1 $aBUENO, L. A.
700 1 $aLOBO JUNIOR, M.
700 1 $aMASCARIN, G. M.
700 1 $aFINKLER, C. L. L.
773 $tBiocatalysis and Agricultural Biotechnology$gv. 44, article 102455, 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 7 | |
| 1. |  | SUDANO, M. J.; PASCHOAL, D. M.; RASCADO, T. S.; MAGALHÃES, L. C. O.; CROCOMO, L. F.; LIMA NETO, J. F.; MACHADO, R.; LADIM-ALVARENGA, F. C. Phenazine ethosulfate effect in the development and lipid content of in vitro produced bovine embryos. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro. Abstracts... São Pedro: CBRA, 2010| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 2. | | SUDANO, M. J; PASCHOAL, D. M.; RASCADO, T. S.; MAGALHÃES, L. C. O.; CROCOMO, L. F.; LIMA NETO, J. F.; MACHADO, R.; LANDIM ALVARENGA, F. da C. Phenazine ethosulfate and fetal calf serum effects on the development and apoptosis in vitro produced bovne embryos. Reproduction Fertility and Development, v. 23, p. 173, 2011.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 3. | | SUDANO, M. J.; PASCHOAL, D. M.; MAZIERO, R. R. D.; RASCADO, T. S.; GUASTALLI, M. D.; CROCOMO, L. F.; MAGALHÃES, L. C. O.; MONTEIRO, B. A.; MARTINS JR. A.; MACHADO, R.; LANDIM-ALVARENGA, F. D. C. Improving postcryopreservation survival capacity: an embryo-focused approach. Anim. Reprod., v.10, n.3, p.160-167, Jul./Sept. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | SUDANO, M. J.; PASCHOAL, D. M.; MAZIERO, R. R. D.; RASCADO, T. S.; GUASTALI, M. D.; CROCOMO, L. F.; MAGALHÃES, L. C. O.; MONTEIRO, B. A.; MARTINS JUNIOR, A.; MACHADO, R.; LANDIM-ALVARENGA, F. D. C. Improving posteryopreservation survival capacity: an embryo approach. In: 27th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, v.10, n.3, p.368, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | SUDANO, M. J.; PASCHOAL, D. M.; RASCADO, T. S.; GUASTALI, M. D.; MAGALHÃES, L. C. O; CROCOMO, L. F.; LIMA NETO, J. F. de; MACHADO, R.; LANDIM-ALVARENGA, F. C. Determinants survival aspects of vitrified in vitro produced bovine embryos. In: ANNUAL MEETING OF THE SOCIETY FOR THE STUDY OF REPRODUCTION, 44., 2011, Portland. Proceedings... Portland: Biology of Reproduction, 2011. p. 60| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | SUDANO, M. J.; CAIXETA, E. S.; PASCHOAL, D. M.; RASCADO, T. S.; CROCOMO, L. F.; MAZIERO, R. R.; MAGALHÃES, L. C. O.; GUASTALI, M. D.; VERGARA, L. E.; MONTEIRO, B. A.; MARTINS JUNIOR, A.; MACHADO, R.; BURATINI, J.; LANDIN-ALVARENGA, F. C. Global gene expression patterns of fresh and vitrified in vitro-produced bovine blastocysts. In: Reproduction, Fertility and Development, p. 187, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | SUDANO, M. J.; PASCHOAL, D. M.; CAIXETA, E. S.; MAZIERO, R. R. D.; GUASTALI, M. D.; CROCOMO, L. F.; RASCADO, T. S.; MAGALHÃES, L. C. O.; MONTEIRO, B. A.; MARTINS JÚNIOR, A.; MACHADO, R.; BURATINI, J.; LANDIM-ALVARENGA, F. D. C. Effect of high fetal calf serum concentration in the gene expression pattern of in vitro produced bovine embryos. Reproduction, Fertility and Development, v. 26, p. 155, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 7 | |
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