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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
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Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
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Páginas: |
p. 134-141 |
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ISSN: |
0093-691X |
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Idioma: |
Português |
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Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
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Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
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Thesagro: |
Cultura de Embrião; Embrião. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02843naa a2200349 a 4500
001 2127986
005 2020-12-11
008 2021 bl uuuu u00u1 u #d
022 $a0093-691X
100 1 $aMARQUES, T. C.
245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource]
260 $c2021
300 $ap. 134-141
520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance.
650 $aCultura de Embrião
650 $aEmbrião
653 $aApoptose celular
653 $aCriotolerância
653 $aFertilização
653 $aMelatonina
653 $aVitrificação
700 1 $aSANTOS, E. C. da S.
700 1 $aDIESEL, T. O.
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aLEME, L. de O.
700 1 $aDODE, M. A. N.
700 1 $aALVES, B. G.
700 1 $aCOSTA, F. P. H.
700 1 $aOLIVEIRA, E. B.
700 1 $aGAMBARINI, M. L.
773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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Embrapa Cerrados (CPAC) |
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| Registros recuperados : 162 | |
| 3. |  | PEREZ, K. S. S.; RAMOS, M. L. G.; McMANUS, C. Nitrogênio da biomassa microbiana em solo cultivado com soja, sob diferentes sistemas de manejo, nos Cerrados. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 2, p. 137-144, fev. 2005 Título em inglês: Microbial biomass nitrogen in soil cultivated with soybean, under different management systems, in the Cerrado.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | PEREZ, K. S. S.; RAMOS, M. L. G.; McMANUS, C. Carbono da biomassa microbiana em solo cultivado com soja sob diferentes sistemas de manejo nos Cerrados. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 6, p. 567-573, junho 2004 Título em inglês: Microbial biomass carbon in soil cultivated with soybean, under different management systems in Cerrado.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | MACHADO, L. de L.; RAMOS, M. L. G.; CALDAS, L. S.; VIVALDI, L. J. Seleção de matrizes e clones de mangabeira para o cultivo in vitro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 5, p. 431-435, maio 2004 Título em inglês: Selection of parents and clones of mangabeira for in vitro cultivation.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | COSER, T. R.; FIGUEIREDO, C. C.; RAMOS, M. L. G.; JANNUZZI, H.; MARCHAO, R. L. Recuperação de carbono obtida por três métodos em frações da matéria orgânica de Latossolo, sob consórcio milho-forrageiras, no Cerrado. Bioscience Journal, v. 28, supl. 1, p. 91-97, mar. 2012. p. 91-97| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | COSER, T. R.; RAMOS, M. L. G.; AMABILE, R. F.; RIBEIRO JÚNIOR, W. Q. Nitrogênio da biomassa microbiana em solo de Cerrado com aplicação de fertilizante nitrogenado. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 3, p. 399-406, mar. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Cerrados; Embrapa Trigo; Embrapa Unidades Centrais. |
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| 16. | | FERREIRA, E. A. B.; RESCK, D. V.; RAMOS, M. L. G.; GOMES, A. C. Dinamica do carbono da biomassa microbiana em cinco epocas do ano sob diferentes sistemas de manejo do solo no cerrado. In: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 25.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 9.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 7.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 4., 2002, Rio de Janeiro. FertBio 2002: agricultura: bases ecologicas para o desenvolvimento social e economico sustentado. Rio de Janeiro: [s. n.], 2002. nao paginado.| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | RAMOS, M. L. G.; SILVA, S. L.; VARGAS, M. A. T. Compatibility between Rhizobium strains and recommended fungicides for common bean (Phaseolus vulgaris L.). In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity - proceedings. Dordrechet: Kluwer, 2000. p.612. (Current Plant Science and Biotechnology in Agriculture, v.38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 162 | |
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