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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
01/03/2011 |
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Data da última atualização: |
04/03/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MARRACINI, P.; RAMOS, H. J. O.; VIEIRA, L. G. E.; FERRAO, M. A. G.; SILVA, F. R. da; MELO, J. A. T.; BLOCH JUNIOR, C.; ANDRADE, A. C. |
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Afiliação: |
IAPAR; IAPAR; MARIA AMELIA GAVA FERRAO, SAPC; JORGE ALEX TAQUITA MELO, CENARGEN; CARLOS BLOCH JUNIOR, CENARGEN; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Study of drought-tolerance mechanisms in coffee plants by an integrated analysis. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
The principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to indentify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. |
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Thesagro: |
Clone; Coffea Canephora. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01831nam a2200217 a 4500
001 1880084
005 2011-03-04
008 2008 bl uuuu u00u1 u #d
100 1 $aMARRACINI, P.
245 $aStudy of drought-tolerance mechanisms in coffee plants by an integrated analysis.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008
520 $aThe principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to indentify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed.
650 $aClone
650 $aCoffea Canephora
700 1 $aRAMOS, H. J. O.
700 1 $aVIEIRA, L. G. E.
700 1 $aFERRAO, M. A. G.
700 1 $aSILVA, F. R. da
700 1 $aMELO, J. A. T.
700 1 $aBLOCH JUNIOR, C.
700 1 $aANDRADE, A. C.
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Embrapa Café (CNPCa) |
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| 7. |  | PRESTES, R. A.; COLNAGO, L. A.; CARRILHO, E.; BASSANEZZI, R. B.; FORATO, L. A. Análises metabolômicas de plantas com morte súbita do citrus. In: CONGRESSO PAULISTA DE FITOPATOLOGIA, 27., Campinas. Summa Phytopatologica, Jaguariúna, v.30, n.1, p. 78, 2004.| Biblioteca(s): Embrapa Instrumentação. |
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| 11. | | PRESTES, R. A.; COLNAGO, L. A.; FORATO, L. A.; VIZZOTTO, L.; NOVOTNY, E. H.; CARRILHO, E. A rapid and automated low resolution NMR method to analyze oil quality in intact oilseeds. Analytica Chimica Acta, Amsterdam, v. 596, n. 2, p. 325-329, July 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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| 12. | | PRESTES, R. A.; COLNAGO, L. A.; FORATO, L. A.; VIZZOTTO, L.; NOVOTNY, E. H.; CARRILHO, E. A rapid and automated low resolution NMR method to analyze oil quality in intact oilseeds. Analytica Chimica ACTA, Amsterdam, v. 596, p. 325-329, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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| 13. | | PRESTES, R. A.; COLNAGO, L. A.; FORATO, L. A.; CARRILHO, E.; BASSANEZI, R. B.; WULFF, N. A. Nuclear magnetic resonance characterization of metabolite disorder in orange trees caused by citrus sudden death disease. Molecular plant pathology, [S. l.], v. 10, n. 1, p. 51-57, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| Registros recuperados : 13 | |
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