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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Rondônia. |
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Data corrente: |
03/04/2012 |
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Data da última atualização: |
10/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PFEIFER, L. F. M.; SIQUEIRA, L. G. B.; ADAMS, G. P.; PIERSON, R. A.; SINGH, J. |
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Afiliação: |
LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; G.P. Adams, Department of Veterinary Biomedical Sciences, University of Saskatchewan; R.A. Pierson, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Saskatchewan; J. Singh, Department of Veterinary Biomedical Sciences, University of Saskatchewan. |
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Título: |
In vivo imaging of cumulus-oocyte-complexes and small ovarian follicles in cattle using ultrasonic biomicroscopy. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Animal Reproduction Science, Amsterdam, v. 131, p. 88-94, 2012. |
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ISSN: |
0378-4320 |
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Idioma: |
Inglês |
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Notas: |
doi:10.1016/j.anireprosci.2012.02.014 |
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Conteúdo: |
The objectives of this study were to: (1) evaluate the feasibility of ultrasonic biomicroscopy (UBM) for imaging ovarian follicles in vivo in cows and heifers, (2) compare the transvaginal to the transrectal approach, (3) compare the number of follicles detected by UBM to conventional ultrasonography (US), and (4) identify cumulus-oocyte-complexes (COC) within follicles by UBM. Mature cows (n = 5) and peri-pubertal heifers (n = 5) were examined once using conventional B-mode US (Aloka 900) with a 7.5 MHz transrectal, and a 5 MHz transvaginal transducers. A second series of examinations was performed using UBM (Visualsonics Vevo 660) equipped with a 30 MHz transrectal and a 40 MHz transvaginal transducer. A three- to four-fold increase in the number of small follicles (<3 mm) was detected using the transvaginal approach with UBM compared to conventional US in both heifers (32.4 ± 4.24 compared to 7.2 ± 1.4; P < 0.0001) and cows (35.0 ± 13.8 compared to 10.7 ± 7.5; P = 0.0013). However, fewer follicles (all sizes combined) were detected using the transrectal approach with UBM compared to conventional US in both heifers (8.6 ± 5.6 compared to 17.6 ± 4.9; P = 0.02) and cows (5.3 ± 6.1 compared to 20.3 ± 7; P = 0.04). In heifers, COC-like structures were identified in 39 of 202 (19.3%) follicles examined. In conclusion, UBM using a transvaginal approach is feasible and may be used for in vivo assessment of early antral follicles as small as 0.4 mm, and COC within follicles. |
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Palavras-Chave: |
Biomicroscope; Biomicroscopia; Biomicroscopico; Foliculos ovarianos; Imagem; Imaging; Oocito; Oocyte; Ultrasonografia animal; Ultrassonografia. |
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Thesaurus Nal: |
ovarian follicles; ultrasonography. |
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Categoria do assunto: |
G Melhoramento Genético
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02493naa a2200337 a 4500
001 1921516
005 2021-11-10
008 2012 bl uuuu u00u1 u #d
022 $a0378-4320
100 1 $aPFEIFER, L. F. M.
245 $aIn vivo imaging of cumulus-oocyte-complexes and small ovarian follicles in cattle using ultrasonic biomicroscopy.$h[electronic resource]
260 $c2012
500 $adoi:10.1016/j.anireprosci.2012.02.014
520 $aThe objectives of this study were to: (1) evaluate the feasibility of ultrasonic biomicroscopy (UBM) for imaging ovarian follicles in vivo in cows and heifers, (2) compare the transvaginal to the transrectal approach, (3) compare the number of follicles detected by UBM to conventional ultrasonography (US), and (4) identify cumulus-oocyte-complexes (COC) within follicles by UBM. Mature cows (n = 5) and peri-pubertal heifers (n = 5) were examined once using conventional B-mode US (Aloka 900) with a 7.5 MHz transrectal, and a 5 MHz transvaginal transducers. A second series of examinations was performed using UBM (Visualsonics Vevo 660) equipped with a 30 MHz transrectal and a 40 MHz transvaginal transducer. A three- to four-fold increase in the number of small follicles (<3 mm) was detected using the transvaginal approach with UBM compared to conventional US in both heifers (32.4 ± 4.24 compared to 7.2 ± 1.4; P < 0.0001) and cows (35.0 ± 13.8 compared to 10.7 ± 7.5; P = 0.0013). However, fewer follicles (all sizes combined) were detected using the transrectal approach with UBM compared to conventional US in both heifers (8.6 ± 5.6 compared to 17.6 ± 4.9; P = 0.02) and cows (5.3 ± 6.1 compared to 20.3 ± 7; P = 0.04). In heifers, COC-like structures were identified in 39 of 202 (19.3%) follicles examined. In conclusion, UBM using a transvaginal approach is feasible and may be used for in vivo assessment of early antral follicles as small as 0.4 mm, and COC within follicles.
650 $aovarian follicles
650 $aultrasonography
653 $aBiomicroscope
653 $aBiomicroscopia
653 $aBiomicroscopico
653 $aFoliculos ovarianos
653 $aImagem
653 $aImaging
653 $aOocito
653 $aOocyte
653 $aUltrasonografia animal
653 $aUltrassonografia
700 1 $aSIQUEIRA, L. G. B.
700 1 $aADAMS, G. P.
700 1 $aPIERSON, R. A.
700 1 $aSINGH, J.
773 $tAnimal Reproduction Science, Amsterdam$gv. 131, p. 88-94, 2012.
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| 4. |  | SIGNOR, L. R.; PIVOTTO, A. C.; FONTANELI, R. S.; FONTANELI, R. S.; SANTOS, H. P. dos. Sobressemeadura de forrageiras de inverno em pastagem de Tifton 85 (Cynodon sp.). In: MOSTRA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA TRIGO, 5., 2009, Passo Fundo. Resumos... Passo Fundo: Embrapa Trigo, 2009. 1 p. html. (Embrapa Trigo. Documentos online, 115).| Tipo: Resumo em Anais de Congresso |
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| 5. | | FONTANELI, R. S.; FONTANELI, R. S.; MARIANI, F.; PIVOTTO, A. C.; SIGNORI, L. R. Gramíneas perenes de verão. In: FONTANELI, REN. S.; SANTOS, H. P. dos; FONTANELI, ROB. S. (Ed.). Forrageiras para integração lavoura-pecuária-floresta na região sul-brasileira. Passo Fundo: Embrapa Trigo, 2009. Cap. 11, p. 199-238.| Tipo: Capítulo em Livro Técnico-Científico |
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