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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/06/2025 |
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Data da última atualização: |
25/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, J. O. S.; ALVARENGA, A. D.; CORREA, D. S.; MERCANTE, L. A.; SANTANA, R. M. M. |
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Afiliação: |
FEDERAL UNIVERSITY OF BAHIA (UFBA); DANIEL SOUZA CORREA, CNPDIA. |
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Título: |
Blown spun fibers-based colorimetric sensor for detecting trace zinc in seminal fluid. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Biosensors and Bioelectronics, v. 270, 116943, 2025. |
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Páginas: |
10 p. |
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ISSN: |
0956-5663 |
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DOI: |
https://doi.org/10.1016/j.bios.2024.116943 |
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Idioma: |
Inglês |
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Conteúdo: |
Rapid and accurate analysis of zinc in seminal fluid is an important tool for male infertility diagnosis and forensic investigation of sexual assault. Among the traditional techniques employed for this purpose, colorimetric solidstate sensors offer a simple and efficient alternative for screening this analyte. Herein, we evaluated the feasibility of using solution blow spinning as a fast, simple, and cost-effective method to develop porous membranes that can be manufactured as low-cost colorimetric solid-state sensing materials. Specifically, we report a one-step approach to prepare blown spun polylactic acid (PLA)/poly(ethylene oxide) (PEO) fibrous membranes modified with 1-(2-pyridylazo)-2-naphthol (PAN) for the quantitative and qualitative screening of Zn2+ in human seminal fluid. The fibrous membranes were fully characterized and then used to prepare discs and swabs. By digital image colorimetry, a spot-like assay was established using PLA/PEO@PAN membrane discs, which under optimized conditions, exhibited a linear range of up to 15 mg L− 1 , a limit of detection of 0.06 mg L− 1 and high selectivity in the presence of several interfering species commonly found in seminal fluid. Additionally, the sensor was successfully applied in the detection of Zn2+ in human semen samples, showing no significant differences compared to the standard method. Furthermore, PLA/PEO@PAN swabs were employed in a naked-eye presumptive test to identify semen stains, showing satisfactory visual response even in the presence of only 0.11 μg of zinc. Moreover, the swabs’ color changes were highly unambiguous, revealing a concordance rate of almost 90% in a blind study. This outstanding performance can be ascribed to the high porosity and wettability of the fibrous membrane and the PAN’s coordination ability towards Zn2+. Overall, the remarkable advantages achieved with our approach lie in the easy production, on-site, and cost-effective analysis of zinc as a biomarker for clinical and forensic applications. MenosRapid and accurate analysis of zinc in seminal fluid is an important tool for male infertility diagnosis and forensic investigation of sexual assault. Among the traditional techniques employed for this purpose, colorimetric solidstate sensors offer a simple and efficient alternative for screening this analyte. Herein, we evaluated the feasibility of using solution blow spinning as a fast, simple, and cost-effective method to develop porous membranes that can be manufactured as low-cost colorimetric solid-state sensing materials. Specifically, we report a one-step approach to prepare blown spun polylactic acid (PLA)/poly(ethylene oxide) (PEO) fibrous membranes modified with 1-(2-pyridylazo)-2-naphthol (PAN) for the quantitative and qualitative screening of Zn2+ in human seminal fluid. The fibrous membranes were fully characterized and then used to prepare discs and swabs. By digital image colorimetry, a spot-like assay was established using PLA/PEO@PAN membrane discs, which under optimized conditions, exhibited a linear range of up to 15 mg L− 1 , a limit of detection of 0.06 mg L− 1 and high selectivity in the presence of several interfering species commonly found in seminal fluid. Additionally, the sensor was successfully applied in the detection of Zn2+ in human semen samples, showing no significant differences compared to the standard method. Furthermore, PLA/PEO@PAN swabs were employed in a naked-eye presumptive test to identify semen stains, showing satisfactory visual ... Mostrar Tudo |
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Palavras-Chave: |
Clinical analysis; Digital image colorimetry; Fiber swabs; Solid-state sensors; Solution blow spinning. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02835naa a2200265 a 4500
001 2176746
005 2025-06-25
008 2025 bl uuuu u00u1 u #d
022 $a0956-5663
024 7 $ahttps://doi.org/10.1016/j.bios.2024.116943$2DOI
100 1 $aSILVA, J. O. S.
245 $aBlown spun fibers-based colorimetric sensor for detecting trace zinc in seminal fluid.$h[electronic resource]
260 $c2025
300 $a10 p.
520 $aRapid and accurate analysis of zinc in seminal fluid is an important tool for male infertility diagnosis and forensic investigation of sexual assault. Among the traditional techniques employed for this purpose, colorimetric solidstate sensors offer a simple and efficient alternative for screening this analyte. Herein, we evaluated the feasibility of using solution blow spinning as a fast, simple, and cost-effective method to develop porous membranes that can be manufactured as low-cost colorimetric solid-state sensing materials. Specifically, we report a one-step approach to prepare blown spun polylactic acid (PLA)/poly(ethylene oxide) (PEO) fibrous membranes modified with 1-(2-pyridylazo)-2-naphthol (PAN) for the quantitative and qualitative screening of Zn2+ in human seminal fluid. The fibrous membranes were fully characterized and then used to prepare discs and swabs. By digital image colorimetry, a spot-like assay was established using PLA/PEO@PAN membrane discs, which under optimized conditions, exhibited a linear range of up to 15 mg L− 1 , a limit of detection of 0.06 mg L− 1 and high selectivity in the presence of several interfering species commonly found in seminal fluid. Additionally, the sensor was successfully applied in the detection of Zn2+ in human semen samples, showing no significant differences compared to the standard method. Furthermore, PLA/PEO@PAN swabs were employed in a naked-eye presumptive test to identify semen stains, showing satisfactory visual response even in the presence of only 0.11 μg of zinc. Moreover, the swabs’ color changes were highly unambiguous, revealing a concordance rate of almost 90% in a blind study. This outstanding performance can be ascribed to the high porosity and wettability of the fibrous membrane and the PAN’s coordination ability towards Zn2+. Overall, the remarkable advantages achieved with our approach lie in the easy production, on-site, and cost-effective analysis of zinc as a biomarker for clinical and forensic applications.
653 $aClinical analysis
653 $aDigital image colorimetry
653 $aFiber swabs
653 $aSolid-state sensors
653 $aSolution blow spinning
700 1 $aALVARENGA, A. D.
700 1 $aCORREA, D. S.
700 1 $aMERCANTE, L. A.
700 1 $aSANTANA, R. M. M.
773 $tBiosensors and Bioelectronics$gv. 270, 116943, 2025.
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Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 121 | |
| 10. |  | PUTI, F. da C.; BECARO, A. A.; PILON, L.; CORREA, D. S.; FERREIRA, M. D. Aplicação de filmes nanoparticulados na conservação de morangos. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 112-113 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | PILON, L.; SPRICIGO, P. C.; MOURA, M. R.; MATTOSO, L. H. C.; FERREIRA, M. D. Evaluación del pardeamiento enzimático de manzanas mínimamente procesadas recubiertas com nanopartículas de quitosano. In: CONGRESO IBEROAMERICANO DE TECNOLOGIA POSTCOSECHA Y AGROEXPORTACIONES, 7., 2012, La Plata, Argentina. Resumos...La Plata, Argentina: AITEP, 2012. 1 CD-ROM. não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | PILON, L.; SPRICIGO, P. C.; MOURA, M. R.; MATTOSO, L. H. C.; FERREIRA, M. D. Efectos de los revestimientos comestibles a base de nanopartículas de quitosano em la composición gaseosa de manzanas mínimamente procesadas. In: CONGRESO IBEROAMERICANO DE TECNOLOGIA POSTCOSECHA Y AGROEXPORTACIONES, 7., 2012, La Plata, Argentina. Resumos...La Plata, Argentina: AITEP, 2012. 1 CD-ROM. não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | OHASHI, T. L.; PILON, L.; SPRICIGO, P. C.; MIRANDA, M.; FERREIRA, M. D. Efeito do revestimento de cera de carnaúba em mamões 'Golden'. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 91-93 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | PILON, L.; SPRICIGO, P. C.; MOURA, M. R.; MATTOSO, L. H. C.; FERREIRA, M. D. Efeitos de revestimento à base de nanopartículas de quitosana na atividade enzimática de maçãs minimamente processadas. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 94-96 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | SOUZA, D. G.; RESENDE, O.; PILON, L.; CABRAL, J. C. O.; RESENDE, L. F. L. Caracterização físico-química e tecnológica de farinha de batata-doce biofortificada seca em diferentes temperaturas. Research, Society and Development, v. 9, n. 11, e6339110386, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 121 | |
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