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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
14/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, S. O. D. de; SAYD, R. M.; BALZON, T. A.; SCHERWINSKI PEREIRA, J. E. |
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Afiliação: |
Sharrine Omari Domingues de Oliveira, Universidade Federal de Viçosa; Ricardo Meneses Sayd, UnB; Talita Aparecida Balzon, Universidade Federal do Amazonas; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Scientia Horticulturae, v. 161, p. 204-209, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures. MenosWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results ind... Mostrar Tudo |
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Palavras-Chave: |
Shoot proliferation. |
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Thesagro: |
Micropropagação. |
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Thesaurus Nal: |
micropropagation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02408naa a2200193 a 4500
001 2067485
005 2024-06-14
008 2013 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, S. O. D. de
245 $aA new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system.$h[electronic resource]
260 $c2013
520 $aWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures.
650 $amicropropagation
650 $aMicropropagação
653 $aShoot proliferation
700 1 $aSAYD, R. M.
700 1 $aBALZON, T. A.
700 1 $aSCHERWINSKI PEREIRA, J. E.
773 $tScientia Horticulturae$gv. 161, p. 204-209, 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 13 | |
| 1. |  | BALIEIRO, F. de C.; MORAES, L. F. D. de; PRADO, R. B.; MOURA, C. J. R. de; SANTOS, F. M.; PEREIRA, A. P. de A. Ecosystem services in eucalyptus planted forests and mixed and multifunctional planted forests. In: CARDOSO, E.J. B. N.; GONÇALVES, J. L. de M.; BALIEIRO, F. de C.; FRANCO, A. A. (Eds.) Mixed plantations of eucalyptus and leguminous trees. Basel: Springer, 2020. p. 193-219| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 2. | | BALIEIRO, F. de C.; MORAES, L. F. D. de; PRADO, R. B.; MOURA, C. J. R. de; SANTOS, F. M.; PEREIRA, A. P. de A. Ecosystem services in Eucalyptus planted forests and mixed and multifunctional planted forests. In: CARDOSO, E. J. B. N.; GONÇALVES, J. L. de M.; BALIEIRO, F. de C.; FRANCO, A. A. (ed.). Mixed plantations of Eucalyptus and leguminous trees: soil, microbiology and ecosystem services. Cham: Springer, 2020. cap. 10, p. 193-219.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
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| 3. | | CAMPOS. A. S. N.; JESUS, E. da C.; MENDES FILHO, P. F.; PEREIRA, A. P. de A.; CASTRO, A. C. R. de; COELHO, A. C. M. A.; BORDALLO, P. do N.; BORGES, W. L.; CARVALHO, A. C. P. P. de. Soil quality changes in cashew orchard production in Brazilian semiarid areas: a comprehensive field survey across locations, seasons, tree types, and management practices. Applied Soil Ecology, v. 211, 106102, 2025. 0929-1393| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Agroindústria Tropical. |
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| 4. | | LIMA, A. R. de S.; FREITAS, L. M. de; AMARAL, L. M. R.; RODRIGUES, A. L.; PEREIRA, A. P. de A.; CARVALHO, A. C. P. P. de; TANIGUCHI, C. A. K.; SILVA, C. de F. B. da; SAGGIN JUNIOR, O. J.; SILVA, E. M. R. da; BORGES, W. L. Acúmulo de nutrientes em bananeiras inoculadas com fungos micorrízicos arbusculares. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AGROINDÚSTRIA TROPICAL, 19., 2022, Fortaleza. Resumos... Fortaleza: Embrapa Agroindústria Tropical, 2023. p. 16.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 5. | | SILVA, A. M. M.; FEILER, H. P.; LACERDA-JÚNIOR, G. V.; FERNANDES JUNIOR, P. I.; AIDAR, S. de T.; ARAÚJO, V. A. V. P. de; MATTEOLI, F. P.; PEREIRA, A. P. de A.; MELO, I. S. de; CARDOSO, E. J. B. N. Arbuscular mycorrhizal fungi associated with the rhizosphere of an endemic terrestrial bromeliad and a grass in the Brazilian neotropical dry forest. Brazilian Journal of Microbiology, v, 54, n. 3, p. 1955-1967, Sept. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Semiárido. |
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| 6. | | COSTA, R. M.; ROCHA, S. M. B.; VENTURA, S. H.; AVIZ, R. O. de; LEITE, M. R. L.; SOUZA, H. A. de; ALCANTARA NETO, F. de; MEDEIROS, E. V. de; PEREIRA, A. P. de A.; MENDES, L. W.; ARAÚJO, A. S. F. Differential recruitment of plant growth-promoting bacteria community by soybean rhizosphere in no-tillage and integrated crop-livestock. Rhizosphere, v. 31, 100948, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 7. | | COSTA, R. M.; ARAUJO, E. M. B.; SILVA, D. E. O.; ROCHA, S. M. B.; BONIFACIO, A.; SOUSA, R. S.; PEREIRA, A. P. de A.; MEDEIROS, E. V. de; SAGRILO, E.; OLIVEIRA JUNIOR, J. O. L. de; SOUZA, H. A. de; ARAUJO, A. S. F. Seasonal responses of soil microbial biomass C and enzymatic activity comparing no-tillage and integrated crop-livestock systems. European Journal of Soil Biology, v. 121, 103628, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 8. | | COSTA, R. M.; COSTA, M. K. L.; ROCHA, S. M. B.; LEITE, M. R. L.; ALCANTARA NETO, F. de; SOUZA, H. A. de; PEREIRA, A. P. de A.; MELO, V. M. M.; MEDEIROS, E. V. de; MENDES, L. W.; ARAUJO, A. S. F. Soil management shapes bacterial and archaeal communities in soybean rhizosphere: Comparison of no-tillage and integrated crop-livestock systems. Rhizosphere, v. 30, 100886, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 9. | | GUALBERTO, A. V. S.; SOUZA, H. A. de; SAGRILO, E.; ARAUJO, A. S. F.; MENDES, L. W.; MEDEIROS, E. V. de; PEREIRA, A. P. de A.; COSTA, D. P. da; VOGADO, R. F.; CUNHA, J. R. da; TEIXEIRA NETO, M. L.; LEITE, L. F. C. Organic C fractions in topsoil under different management systems in Northeastern Brazil. Soil Systems, v. 7, n. 11, p. 1-13, Feb. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 10. | | ARAÚJO, V. L. V. P. de; LIRA JUNIOR, M. A.; SOUZA JÚNIOR, V. S. de; ARAUJO FILHO, J. C. de; FRACETTO, F. J. C.; ANDREOTE, F. D.; PEREIRA, A. P. de A.; MENDES JÚNIOR, J. P.; BARROS, F. M. do R.; FRACETTO, G. G. M. Bacteria from tropical semiarid temporary ponds promote maize growth under hydric stress. Microbiological Research, v. 240, 126564, Nov. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Solos. |
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| 11. | | ALBUQUERQUE, T. M. de; MENDES, L. W.; ROCHA, S. M. B.; ANTUNES, J. E. L.; OLIVEIRA, L. M. de S.; MELO, V. M. M.; OLIVEIRA, F. A. S.; PEREIRA, A. P. de A.; SILVA, V. B. da; GOMES, R. L. F.; ALCANTARA NETO, F. de; LOPES, A. C. de A.; ROCHA, M. de M.; ARAUJO, A. S. F. Genetically related genotypes of cowpea present similar bacterial community in the rhizosphere. Scientific Reports, v. 12, 3472, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | BARBOSA, F. L. A.; SANTOS, J. M. R.; MOTA, J. C. A.; COSTA, M. C. G.; ARAUJO, A. S. F.; GARCIA, K. G. V.; ALMEIDA, M. S.; NASCIMENTO, I. V.; MEDEIROS, E. V.; FERREIRA, O. P.; SOUZA FILHO, A. G.; FREGOLENTE, L. G.; SOUSA, H. F.; BORGES, W. L.; PEREIRA, A. P. DE A. Potential of biochar to restoration of microbial biomass and enzymatic activity in a highly degraded semiarid soil. Scientific Reports, 14: 26065, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 13. | | SILVA, W. J. dos S.; ALCANTARA NETO, F. de; AL-QAHTANI, W. H.; OKLA, M. K.; AL-HASHIMI, A.; VIEIRA, P. F. de M. J.; GRAVINA, G. de A.; ZUFFO, A. M.; DUTRA, A. F.; CARVALHO, L. C. B.; SOUSA, R. S. de; PEREIRA, A. P. de A.; LEITE, W. de S.; SILVA JUNIOR, G. B. da; SILVA, A. C. da; LEITE, M. R. L.; LUSTOSA SOBRINHO, R.; ABDELGAWAD, H. Yield of soybean genotypes identified through GGE biplot and path analysis. PLOS ONE, v. 17, n. 10, e0274726, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 13 | |
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