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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2017 |
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Data da última atualização: |
03/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
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ISSN: |
1572-9699 |
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DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
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Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
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Thesaurus Nal: |
nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02682naa a2200265 a 4500
001 2083708
005 2018-01-03
008 2017 bl uuuu u00u1 u #d
022 $a1572-9699
024 7 $ahttps://doi.org/10.1007/s10482-017-0906-7$2DOI
100 1 $aPOLESE, V.
245 $aSelection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.$h[electronic resource]
260 $c2017
520 $aQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism.
650 $anitrogen fixation
653 $aInternal normalizing
653 $aOrganic acids
653 $aRelative expression
653 $aSugar transport
700 1 $aSOARES, C. de P.
700 1 $aSILVA, P. R. A. da
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tAntonie van Leeuwenhoekv, 110$gn. 12, p. 1555-1568, 2017.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| 2. |  | OLIVEIRA JUNIOR, J. C. de; BEIRIGO, R. M.; CHIAPINI, M.; NASCIMENTO, A. F. do; COUTO, E. G.; VIDAL-TORRADO, P. Origin of mounds in the Pantanal wetlands: An integrated approach between geomorphology, pedogenesis, ecology and soil micromorphology. PLoS One, v. 12, n. 7, e0179197, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 4. | | CHIAPIN, M.; OLIVEIRA JUNIOR, J. C. de O.; NASCIMENTO, A. F. do; FURQUIM, S. A. C.; BEIRIGO, R. M.; VIDAL-TORRADO, P. Caracterização morfológica de solos sódicos em diferentes paleodiques no pantanal norte. In: CONGRESSO BRASILEIRO DE CIÊNCIAS DO SOLO, 34., 2013. Florianópolis. Anais... Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 5. | | SANTOS, L. M. dos; MOTTA, A. C. V.; BOGNOLA, I. A.; OLIVEIRA JUNIOR, J. C. de; PEREIRA, M.; MELLO, A. S. R. de. Correlação entre os índices de vegetação NDVI, VARI e EVI e o volume de madeira em reflorestamento de Pinus taeda. In: CONFERÊNCIA IUFRO 2023 AMÉRICA LATINA, 2023, Curitiba. Anais... Colombo: Embrapa Florestas, 2023. p. 130. (Embrapa Florestas. Eventos técnicos & científicos, 2).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 6. | | CORRÊA, M. M.; BEIRIGO, R. M.; NASCIMENTO, A. F. do; OLIVEIRA JUNIOR, J. C. de; COUTO, E. G.; VIDAL-TORRADO, P. Micromorfologia de Plintossolos do Pantanal-MT. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 34., 2013, Florianópolis. Ciência do solo: para quê e para quem: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 7. | | NASCIMENTO, A. F. do; FURQUIM, S. A. C.; BEIRIGO, R. M.; OLIVEIRA JUNIOR, J. C. de O.; COUTO, E. G.; VIDAL-TORRADO, P. Geoquímica, mineralogia e atributos de gleissolos de dois leques aluviais no Pantanal Norte. In: CONGRESSO BRASILEIRO DE CIÊNCIAS DO SOLO, 34., 2013. Florianópolis. Anais... Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 8. | | NASCIMENTO, A. F. do; FURQUIM, S. A. C.; COUTO, E. G.; BEIRIGO, R. M.; OLIVEIRA JUNIOR, J. C. de O.; CAMARGO, P. B. de; VIDAL-TORRADO, P. Genesis of textural contrasts in subsurface soil horizons in the northern Pantanal-Brazil. Revista Brasileira de Ciências do Solo, v. 37, n. 5, p. 1113-1127, out. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 9. | | SALARO, A. L.; OLIVEIRA JÚNIOR, J. C. de; PONTES, M. D.; NEVES, I. G. A. de A.; FERRAZ, R. B.; HISANO, H.; ZUANON, J. A. S. Replacement of moist ingredients in the feed training of carnivorous fish. Revista Brasileira de Zootecnia, v. 41, n. 10, p. 2294-2298, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 10. | | COUTO, E. G.; OLIVEIRA, V. A.; BEIRIGO, R. M.; OLIVEIRA JUNIOR, J. C. de; NASCIMENTO, A. F. do; VIDAL-TORRADO, P. Solos do pantanal Matogrossense. In: CURI, N.; KER, J. C.; NOVAIS, R. F.; VIDAL-TORRADO, P.; SCHAEFER, C. E. G. R. (Ed.). Pedologia: solos dos biomas brasileiros. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2017. cap. 7, p. 304-352.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 11. | | ZUCON, A. R. S.; PEDREIRA, G. Q.; MOTTA, A. C. V.; GOTZ, L. F.; MAEDA, S.; BASSACO, M. V. M.; MAGRI, E.; PRIOR, S. A.; SOUZA, L. C. de P.; OLIVEIRA JUNIOR, J. C. de. Piling secondary subtropical forest residue: long-term impacts on soil, trees, and weeds. Forests, v. 13, 1183, 15 p., 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 12. | | ERCOLE, T. M.; GOMES, J. B. V.; RODRIGUES, V. dos S.; TRENTIN, N. dos S.; OLIVEIRA JUNIOR, J. C. de; ASSIS-PEREIRA, G.; TOMAZELLO-FILHO, M.; MOURA, A. P. C.; MAEDA, S.; PRIOR, S. A.; CONSALTER, R.; MOTTA, A. C. V. VARI as an indicator of site productivity of Pinus taeda L.: soil, litter, and plant nutrition. European Journal of Forest Research, v. 143, p. 1541-1562, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 12 | |
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