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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/06/2025 |
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Data da última atualização: |
10/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
QUEIROZ, L. N.; MONTEIRO, T. R.; GOMES, A. C. M. M.; CABRAL, G. B.; ARAGÃO, F. J. L. |
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Afiliação: |
LÍDIA N. QUEIROZ, AGROCINCO; TATIANE R. MONTEIRO, UNIVERSIDADE DE BRASÍLIA; ANA CRISTINA MENESES MENDES GOMES, CENARGEN; GLAUCIA BARBOSA CABRAL, CENARGEN; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Enhanced folate content in bioengineered lettuce stably expressed under field conditions. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Transgenic Research, v. 34,20, 2025. |
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DOI: |
https://doi.org/10.1007/s11248-025-00440-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Folate (vitamin B9) is an essential nutrient that plays a crucial role in various bodily functions. Its deficiency can lead to health issues, such as megaloblastic anemia, neural tube defects in the developing fetus and increased risk of cardiovascular diseases. Consequently, there is interest in increasing folate content in food crops by classical and molecular breeding. Since folate is a water-soluble vitamin that is sensitive to heat, we chose to manipulate its synthesis pathway in lettuce, which is often used for salads and sandwiches, without heat treatment, which makes it a good vehicle for making folate available to the population via biofortification. Transgenic lettuce plants were generated to express the GTP-cyclohydrolase I and aminodeoxychorismate synthase coding sequences from Arabidopsis thaliana. Plants were cultivated under greenhouse conditions and two field trials were carried out. Results have shown that transgenic lines presented up to 3.4 times more folate than the non-transgenic lettuce plants and 1.9 times more folate than spinach, considered one of the plants richest in folates. Advanced generations of homozygous plants were cultivated under field conditions for two years and the results showed that folate-biofortification was stable. A folate-biofortified lettuce serving would provide about 36 to 64% of the recommended daily intake. This technology is a foundation to produce folate-biofortified commercial varieties that can help to reduce hidden hunger, decreasing the number of cases of fetus malformations and other diseases. MenosFolate (vitamin B9) is an essential nutrient that plays a crucial role in various bodily functions. Its deficiency can lead to health issues, such as megaloblastic anemia, neural tube defects in the developing fetus and increased risk of cardiovascular diseases. Consequently, there is interest in increasing folate content in food crops by classical and molecular breeding. Since folate is a water-soluble vitamin that is sensitive to heat, we chose to manipulate its synthesis pathway in lettuce, which is often used for salads and sandwiches, without heat treatment, which makes it a good vehicle for making folate available to the population via biofortification. Transgenic lettuce plants were generated to express the GTP-cyclohydrolase I and aminodeoxychorismate synthase coding sequences from Arabidopsis thaliana. Plants were cultivated under greenhouse conditions and two field trials were carried out. Results have shown that transgenic lines presented up to 3.4 times more folate than the non-transgenic lettuce plants and 1.9 times more folate than spinach, considered one of the plants richest in folates. Advanced generations of homozygous plants were cultivated under field conditions for two years and the results showed that folate-biofortification was stable. A folate-biofortified lettuce serving would provide about 36 to 64% of the recommended daily intake. This technology is a foundation to produce folate-biofortified commercial varieties that can help to reduce hidden hung... Mostrar Tudo |
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Palavras-Chave: |
Folate. |
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Thesaurus Nal: |
Biofortification; Folic acid; Genetic transformation; Lettuce; Malnutrition. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02319naa a2200253 a 4500
001 2176536
005 2025-06-10
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-025-00440-1$2DOI
100 1 $aQUEIROZ, L. N.
245 $aEnhanced folate content in bioengineered lettuce stably expressed under field conditions.$h[electronic resource]
260 $c2025
520 $aFolate (vitamin B9) is an essential nutrient that plays a crucial role in various bodily functions. Its deficiency can lead to health issues, such as megaloblastic anemia, neural tube defects in the developing fetus and increased risk of cardiovascular diseases. Consequently, there is interest in increasing folate content in food crops by classical and molecular breeding. Since folate is a water-soluble vitamin that is sensitive to heat, we chose to manipulate its synthesis pathway in lettuce, which is often used for salads and sandwiches, without heat treatment, which makes it a good vehicle for making folate available to the population via biofortification. Transgenic lettuce plants were generated to express the GTP-cyclohydrolase I and aminodeoxychorismate synthase coding sequences from Arabidopsis thaliana. Plants were cultivated under greenhouse conditions and two field trials were carried out. Results have shown that transgenic lines presented up to 3.4 times more folate than the non-transgenic lettuce plants and 1.9 times more folate than spinach, considered one of the plants richest in folates. Advanced generations of homozygous plants were cultivated under field conditions for two years and the results showed that folate-biofortification was stable. A folate-biofortified lettuce serving would provide about 36 to 64% of the recommended daily intake. This technology is a foundation to produce folate-biofortified commercial varieties that can help to reduce hidden hunger, decreasing the number of cases of fetus malformations and other diseases.
650 $aBiofortification
650 $aFolic acid
650 $aGenetic transformation
650 $aLettuce
650 $aMalnutrition
653 $aFolate
700 1 $aMONTEIRO, T. R.
700 1 $aGOMES, A. C. M. M.
700 1 $aCABRAL, G. B.
700 1 $aARAGÃO, F. J. L.
773 $tTransgenic Research$gv. 34,20, 2025.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 343 | |
| 2. |  | NUTTI, M. Entrevista: Marília Nutti. Ilsi Brasil Notícias, ano 19, n. 1, p. 6, jan./abr. 2011.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 4. | | NUTTI, M. R. Os avanços da pesquisa em biofortificação no Brasil. In: REUNIÃO DE BIOFORTIFICAÇÃO NO BRASIL, 4., 2011, Teresina. Palestras e resumos... Rio de Janeiro: Embrapa Agroindústria de Alimentos; Teresina: Embrapa Meio-Norte, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 5. | | NUTTI, M. R. Avaliação de segurança de alimentos geneticamente modificados. Revista Brasileira de Ciências Farmacêuticas, São Paulo, v. 43, p. 123, out. 2007. Suplemento 1. Edição dos resumos do XV Congresso Paulista de Farmacêuticos, VII Seminário Internacional de Farmacêuticos e EXPOFAR 2007, São Paulo, out. 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | NUTTI, M. R. Biofortificação no Brasil. In: SEMINÁRIO FORMAÇÃO DE MULTIPLICADORES, 2010, Rio de Janeiro. Biociências como uma ferramenta para a humanidade. Rio de Janeiro: Associação Nacional de Biossegurança, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | NUTTI, M. R. Biofortificação no Brasil: os projetos HarvestPlus, AgroSalud e BioFORT. In: REUNIÃO ANUAL DE BIOFORTIFICAÇÃO NO BRASIL, 3., 2009, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 148). Resumo também em inglês. Publicado também como: NUTTI, M. R.; SILVA, J. B. C. da; RAMOS, S. R. R.; WATANABE, E.; CARVALHO, J. L. V. de; FUKUDA, W. M. G.; ROCHA, M. M.; DEL PELOSO, M. J. Biofortificação no Brasil: os projetos...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 16. | | NUTTI, M. R. Progress in Brazil in the field of biofortification: Harvest Plus. In: BIOVISION ALEXANDRIA, 2006, Alexandria. Conference booklet. Alexandria: Bibliotheca Alexandrina, [2007]. p. 92. Disponível em: <http://www.bibalex.org/bioalex2006conf/BioVision%20BOOK%203-4.pdf>. Acesso em: 18 fev. 2007.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 17. | | NUTTI, M. R. Rotulagem Nutricional. In: NOVA legislacao comentada de produtos lacteos e de alimentos para fins especiais- diet, light e enriquecidos. Sao Paulo: Fonte Com., 1998. cap. 13, p.185-198.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 18. | | NUTTI, M. R. Rotulagem. In: NOVA legislacao comentada de produtos lacteos e de alimentos para fins especiais- diet, light e enriquecidos. Sao Paulo: Fonte Com., 1998. cap. 12, p.169-184.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 19. | | NUTTI, M. R. Rotulagem e OGM. Deputado Federal Luis Carlos Heinze, 27 ago. 2009.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 20. | | NUTTI, M. R. Strategies to combat micronutrient deficiency in developing countries. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: abstracts. Foz do Iguaçu: IUFoST: ALACCTA, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 343 | |
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