| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
30/03/2022 |
|
Data da última atualização: |
30/03/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, D. M. dos; ANNUNZIO, S. R. de; CARMELLO, J. C.; PAVARINA, A. C.; FONTANA, C. R.; CORREA, D. S. |
|
Afiliação: |
DANIEL SOUZA CORREA, CNPDIA. |
|
Título: |
Combining coaxial electrospinning and 3D printing: design of biodegradable bilayered membranes with dual drug delivery capability for periodontitis treatment. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
ACS Applied Bio Materials, v. 5, 2022. |
|
Páginas: |
146−159 |
|
DOI: |
https://doi.org/10.1021/acsabm.1c01019 |
|
Idioma: |
Português |
|
Conteúdo: |
Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient?s health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core−sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/ tetracycline hydrochloride (TH) as the core and zein/poly(ε-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration. MenosPeriodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient?s health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core−sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/ tetracycline hydrochloride (TH) as the core and zein/poly(ε-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell vi... Mostrar Tudo |
|
Palavras-Chave: |
Asymmetric membrane; Controlled release; Electrospinning; Hierarchical materials; Periodontal membrane. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02757naa a2200265 a 4500
001 2141634
005 2022-03-30
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/acsabm.1c01019$2DOI
100 1 $aSANTOS, D. M. dos
245 $aCombining coaxial electrospinning and 3D printing$bdesign of biodegradable bilayered membranes with dual drug delivery capability for periodontitis treatment.$h[electronic resource]
260 $c2022
300 $a146−159
520 $aPeriodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient?s health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core−sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/ tetracycline hydrochloride (TH) as the core and zein/poly(ε-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration.
653 $aAsymmetric membrane
653 $aControlled release
653 $aElectrospinning
653 $aHierarchical materials
653 $aPeriodontal membrane
700 1 $aANNUNZIO, S. R. de
700 1 $aCARMELLO, J. C.
700 1 $aPAVARINA, A. C.
700 1 $aFONTANA, C. R.
700 1 $aCORREA, D. S.
773 $tACS Applied Bio Materials$gv. 5, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 24 | |
| Registros recuperados : 24 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
