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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/11/2020 |
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Data da última atualização: |
30/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CÔRTES, M. V. de C. B.; OLIVEIRA, M. I. de S.; MATEUS, J. R.; SELDIN, L.; SILVA-LOBO, V. L.; FREIRE, D. M. G. |
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Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MAYTHSULENE INACIO DE SOUSA OLIVEIRA, bolsista CNPAF; JACKELINE ROSSETTI MATEUS, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCY SELDIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VALACIA LEMES DA SILVA LOBO, CNPAF; DENISE MARIA GUIMARAES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biological Control, v. 152, 104460, 2021. |
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ISSN: |
1049-9644 |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2020.104460 |
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Idioma: |
Inglês |
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Conteúdo: |
Use of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling. MenosUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibit... Mostrar Tudo |
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Thesagro: |
Controle Biológico; Mofo Branco; Patógeno. |
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Thesaurus Nal: |
Biological control; Genetics; Genome; Root rot; Sarocladium oryzae. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03540naa a2200301 a 4500
001 2126356
005 2021-04-30
008 2021 bl uuuu u00u1 u #d
022 $a1049-9644
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2020.104460$2DOI
100 1 $aCÔRTES, M. V. de C. B.
245 $aA pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens.$h[electronic resource]
260 $c2021
520 $aUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling.
650 $aBiological control
650 $aGenetics
650 $aGenome
650 $aRoot rot
650 $aSarocladium oryzae
650 $aControle Biológico
650 $aMofo Branco
650 $aPatógeno
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aMATEUS, J. R.
700 1 $aSELDIN, L.
700 1 $aSILVA-LOBO, V. L.
700 1 $aFREIRE, D. M. G.
773 $tBiological Control$gv. 152, 104460, 2021.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 14 | |
| 3. |  | ALVES, J. D.; NACIF, A. de P.; LIVRAMENTO, D. E. do; BARTHOLO, G. F.; GUIMARÃES, P. de T. G. Estudo de maturação do café por estímulo mecânico. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 3., 2003, Porto Seguro. Resumos... Brasília, DF: Embrapa Café, 2003.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 4. | | MEIRELES, E. J. L.; CAMARGO, M. B. P. de; FAHL, J. I.; THOMAZIELLO, R. A.; NACIF, A. de P.; BARDIN, L. Análise do balanço hídrico seqüencial decendial (2002) para a região cafeeira de Mococa, SP. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 3., 2003, Porto Seguro. Resumos... Brasília, DF: Embrapa Café, 2003.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 5. | | BALDOTTO, M. A.; ALVARES, V. H.; FREIRE, F. M.; NEVES, J. C. L.; CANTARUTTI, R. B.; GUIMARÃES, P. T. G.; NACIF, A. de P. Manejo da calagem e gessagem para o cafeeiro em Latossolo Vermelho-Amarelo de Patrocínio, Minas Gerais. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 3., 2003, Porto Seguro. Resumos... Brasília, DF: Embrapa Café, 2003.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 6. | | SOUZA, R. B.; ALVAREZ, V. H. V.; FREIRE, F. M.; GUIMARÃES, P. T. G.; NACIF, A. P.; SALGADO, L. T.; OLIVEIRA, J. A. Manejo da calagem e da gessagem para o cafeeiro. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 2., 2001, Vitória. Anais... Brasília, DF: Embrapa Café, 2002.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 7. | | MEIRELES, E. J. L.; CAMARGO, M. B. P. de; FAHL, J. I.; THOMAZIELLO, R. A.; PEZZOPANE, J. R. M.; NACIF, A. de P.; BARDINI, L. Monitoramento agrometeorológico da cultura do café arábica em Londrina, PR no Ano agrícola 2003-2004. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 8. | | BARBOSA, A. M. da S.; MARTORANO, L. G.; COSTA, D. C.; LISBOA, L. S.; NACIF, A. M. P.; PIMENTEL, M. S. Estimativa do potencial erosivo das chuvas em municípios no entorno a Flona Tapajós, Amazônia. In: SIMPÓSIO DE ESTUDOS E PESQUISAS EM CIÊNCIAS AMBIENTAIS NA AMAZÔNIA, 2., 2013, Belém, PA. [Anais]. [Belém, PA]: Universidade do Estado do Pará, Centro de Ciências Naturais e Tecnologia, 2013. p. 436-440.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | MEIRELES, E. J. L.; CAMARGO, M. B. P. de; FAHL, J. I.; THOMAZIELLO, R. A.; PEZZOPANE, J. R. M.; NACIF, A. de P.; BARDIN, L. Condições agrometeorológicas e fenológicas do cafeeiro arábica nas regiões sul e alto Paranaíba do Estado de Minas Gerais no período de 2002-2004. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 11. | | SANTOS, L. S. dos; MARTORANO, L. G.; COSTA, D. C.; BARBOSA, A. M. S.; MARQUES, M. C.; MORAES, J. R. da S. C. de; BARBOSA, A.; MONTEIRO, S. do N.; NACIF, A.; ROCHA, E. P. da. Simulador do potencial hídrico erosivo em diferentes coberturas do solo: o despertar de práticas agrícolas de baixo carbono na Amazônia. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA PARA O PROGRESSO DA CIÊNCIA, 67., 2015, São Carlos, SP. Resumos... São Paulo: SBPC, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | CARVALHO, C. H. S. de; FAZUOLI, L. C.; GUERREIRO FILHO, O.; PEREIRA, A. A.; ALMEIDA, S. R. de; MATIELLO, J. B.; BARTHOLO, G. F.; SERA, T.; MOURA, W. de M.; MENDES, A. N. G.; REZENDE, J. C. de; FONSECA, A. F. A. da; FERRAO, M. A. G.; FERRÃO, R. G.; NACIF, A. de P.; SILVAROLLA, M. B.; BRAGHINI, M. T. Cultivares de café arábica de porte baixo. In: CARVALHO, C. H. S. de (Ed.). Cultivares de café: origem, características e recomendações. Brasília, DF: Embrapa Café, 2008. 334 p. il. p. 157-226| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Café. |
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| 13. | | VARGAS, E. A.; SILVA, F. B.; SANTOS, E. A.; SOUZA, S. M. C.; SOUZA, S. E.; CORRÊA, T. B. S.; FRANÇA, R. C. A.; AMORIM, S. S.; PFENNING, L. H.; BATISTA, L. R.; PEREIRA, R. T. G.; NOGUEIRA, M. D.; NACIF, A. P.; CESAR JUNIOR, P. Influência do processamento e defeitos do café na incidência e ocorrência de Ocratoxina A. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 14. | | VARGAS, E. A.; SILVA, F. B.; SANTOS, E. A.; SOUZA, S. M.; SOUZA, S. E.; CORRÊA, T. B. S.; FRANÇA, R. C. A.; AMORIM, S. S.; PFENNING, L. H.; BATISTA, L. R.; PEREIRA, R. T. G.; NOGUEIRA, M. D.; NACIF, A. P.; AFONSO JUNIOR, P. C. Influence of coffee processing and defects on the incidence and occurrence of ochratoxin A. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 20., 2004, Bangalore, India. Anais... Montpellier, France: Association for Science and Information on Coffee, 2005. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 14 | |
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