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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/05/2014 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Toxins, v. 6, p. 1598-1614, 2014. |
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DOI: |
10.3390/toxins6051598 |
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Idioma: |
Inglês |
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Conteúdo: |
Bioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. MenosBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activ... Mostrar Tudo |
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Thesagro: |
Toxina. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02484naa a2200217 a 4500
001 1987282
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3390/toxins6051598$2DOI
100 1 $aARGÔLO FILHO, R. C.
245 $aRequirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development.$h[electronic resource]
260 $c2014
520 $aBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems.
650 $aToxina
700 1 $aCOSTA, R. L.
700 1 $aPINHEIRO, H. E.
700 1 $aCORRÊA, F. M.
700 1 $aVALICENTE, F. H.
700 1 $aPOMELLA, A. W. V.
700 1 $aLOGUERCIO, L. L.
773 $tToxins$gv. 6, p. 1598-1614, 2014.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 20 | |
| 5. |  | SILVEIRA, S. da; LAUMANN, R. A.; BORGES, M.; MORAES, M. C. B.; MOURA, J. I. L. Dismorfismo sexual em Conotrachelus Humeropictus (Coleoptera: Curculionidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal, RN. Anais... Natal: Sociedade Brasileira de Entomologia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | MOURA, J. I. L.; CIVIDANES, F. J.; SANTOS FILHO, L. P. dos; VALLE, R. R. Polinização do dendezeiro por besouros no Sul da Bahia. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 3, p. 289-294, mar. 2008 Título em inglês: Pollination of oil palm by weevils in Southern Bahia, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | MOURA, J. I. L.; SANTOS, L. P. dos; BITTENCOURT, M. A. L.; KRUG, C. Preferência do bicudo-das-palmeiras por dendezeiro, caiaué e por seu híbrido interespecífico. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 4, p. 454-456, abr. 2013.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Unidades Centrais. |
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| 11. | | SANTANA, D. L. Q.; PASSOS, E. E. M.; WARWICK, D. R. N.; MOURA, J. I. L.; ROSADO NETO, G. H. Ocorrência de Plectrophoroides incertus voss (Coleoptera: Curculionidae) em coqueiro no Estado do Sergipe. Anais da Sociedade Entomológica do Brasil, Jaboticabal, v. 25, n. 1, p. 145-146, 1996.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | FERREIRA, J. M. S.; LIMA, M. F. de; QUEIROZ, D. L. de; MOURA, J. I. L.; SOUZA, L. A. de. Pragas do coqueiro. In: FERREIRA, J. S. S.; WARWICK, D. R. N.; SIQUEIRA, L. A. (Ed.). A cultura do coqueiro no Brasil. 2. ed. rev. amp. Brasília, DF: SPI; Aracaju: Embrapa CPATC, 1997. c. 8. 40 p. il.; color.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 15. | | TREVISAN, O.; RODRIGUES, M. A. C. de M.; MOURA, J. I. L.; LEMOS, W. de P.; COSTA, J. N. M.; SILVA, N. M. da. Cacau. In: SILVA, N. M. da; ADAIME, R.; ZUCCHI, R. A. (Ed.). Pragas agrícolas e florestais na Amazônia. Brasília, DF: Embrapa, 2016. Pt. 1, cap. 4, p. 73-93.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Rondônia. |
|  |
| 16. | | FERREIRA, J. M. S.; LIMA, M. F. de; QUEIROZ, D. L. de; TEODORO, A. V.; MOURA, J. I. L.; SOUZA, L. A. de. Insetos-praga. In: FERREIRA, J. M. S.; WARNWICK, D. R. N.; SIQUEIRA, L. A. (Ed.). A cultura do coqueiro no Brasil. 3. ed. rev. ampl. Brasilia, DF: EMBRAPA-SPI, 2018. p. 361-428.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Florestas. |
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| 17. | | MOURA, J. I. L.; FERREIRA, J. M. S.; SGRILLO, R. B.; VALLE, R. R.; ALMEIDA, A. A. F. de; CIVIDANES, F. J.; DELABIE, J. H. C. Parisoschoenus obesulus Casey (Coleoptera: Curculionidae) não é praga de frutos novos do coqueiro Neotropical Entomology, Londrina, v. 38, n 2, p. 251-253, [online]. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 18. | | FERREIRA, J. M. S.; LIMA, M. F. de; SANTANA, D. L. de Q.; MOURA, J. I. L.; SOUZA, L. A. de. Pragas do coqueiro. In: FERREIRA, J. M. S.; WARNWICK, D. R. N.; SIQUEIRA, L. A. (Ed.). A cultura do coqueiro no Brasil. 2. ed. rev. ampl. Brasilia, DF: EMBRAPA-SPI; Aracaju: EMBRAPA-CPATC, 1998. p. 189-267.| Biblioteca(s): Embrapa Florestas. |
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| 19. | | MOURA, J. I. L.; OLIVEIRA, M. L. de; LUZ, E. D. M. N.; SILVA, S. D. V. M.; SANTOS, R. S. Weevil associated to gummosis in oil palm (Elaeis guineensis Jacq.) in Brazil. Revista de Agricultura, Piracicaba, v. 88, n. 2, p. 87-89, set. 2013.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Acre. |
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| 20. | | CUNHA, R. N. V. da; GOMES JUNIOR, R. A.; LOPES, R.; PINTO, S. de S.; SILVA, E. B. da; MOURA, J. I. L.; FREITAS, A. F. de; BOARI, A. de J. BRS Manaué: híbrido interespecífico do caiaué com a palma de óleo resistente ao amarelecimento-fatal e ao anel-vermelho. Manaus: Embrapa Amazônia Ocidental, 2023. 10 p. (Embrapa Amazônia Ocidental. Comunicado técnico, 163). ODS.| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
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| Registros recuperados : 20 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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