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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/08/2021 |
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Data da última atualização: |
20/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, M. A. M.; BOMFIM, C. S. G.; RODRIGUES, D. R.; SILVA, A. F. da; SANTOS, J. C. S.; NASCIMENTO, T. R. do; MARTINS, L. M. V.; DANTAS, B. F.; RIBEIRO, P. R. de A.; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I. |
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Afiliação: |
MARCOS ANDRÉ MOURA DIAS, UNIVASF; CLAUDIA SILVA GOMES BOMFIM; DALILA RIBEIRO RODRIGUES, UEPB; ALEKSANDRO FERREIRA DA SILVA, UFRPE; JÉSSICA CAROLINE SOUZA SANTOS, UPE; TAILANE RIBEIRO DO NASCIMENTO, UNEB; LINDETE MÍRIA VIEIRA MARTINS, UNEB; BARBARA FRANCA DANTAS, CPATSA; PAULA ROSE DE ALMEIDA RIBEIRO; ANA DOLORES SANTIAGO DE FREITAS, UFRPE; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Paraburkholderia spp. are the main rhizobial microsymbionts of Mimosa tenuiflora (Willd.) Poir. in soils of the Brazilian tropical dry forests (Caatinga biome). |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Systematic and Applied Microbiology, v. 44, 2021. |
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DOI: |
https://doi.org/10.1016/j.syapm.2021.126208 |
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Idioma: |
Inglês |
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Conteúdo: |
Mimosa tenuiflora (Willd.) Poir. is widespread in southern and central American drylands, but little information is available concerning its associated rhizobia. Therefore, this study aimed to characterize M. tenuiflora rhizobia from soils of the tropical dry forests (Caatinga) in Pernambuco State, Brazil, at the molecular and symbiotic levels. Soil samples of pristine Caatinga areas in four municipalities were used to grow M. tenuiflora. First, the bacteria from root nodules were subjected to nodC/nifH gene amplification, and the bacteria positive for both genes had the 16S rRNA gene sequenced. Then, ten strains were evaluated using recA, gyrB, and nodC gene sequences, and seven of them had their symbiotic efficiency assessed. Thirty-two strains were obtained and 22 of them were nodC/nifH positive. Twenty strains clustered within Paraburkholderia and two within Rhizobium by 16S rRNA gene sequencing. The beta-rhizobia were similar to P. phenoliruptrix (12) and P. diazotrophica (8). Both alpha-rhizobia were closely related to R. miluonense. The recA + gyrB phylogenetic analysis clustered four and five strains within the P. phenoliruptrix and P. diazotrophica branches, respectively, but they were somewhat divergent to the 16S rRNA phylogeny. For Rhizobium sp. ESA 637, the recA + gyrB phylogeny clustered the strain with R. jaguaris. The nodC phylogeny indicated that ESA 626, ESA 629, and ESA 630 probably represented a new symbiovar branch. The inoculation assay showed high symbiotic efficiency for all tested strains. The results indicated high genetic diversity and efficiency of M. tenuiflora rhizobia in Brazilian drylands and included P. phenoliruptrix-like bacteria in the list of efficient beta-rhizobia in the Caatinga biome. MenosMimosa tenuiflora (Willd.) Poir. is widespread in southern and central American drylands, but little information is available concerning its associated rhizobia. Therefore, this study aimed to characterize M. tenuiflora rhizobia from soils of the tropical dry forests (Caatinga) in Pernambuco State, Brazil, at the molecular and symbiotic levels. Soil samples of pristine Caatinga areas in four municipalities were used to grow M. tenuiflora. First, the bacteria from root nodules were subjected to nodC/nifH gene amplification, and the bacteria positive for both genes had the 16S rRNA gene sequenced. Then, ten strains were evaluated using recA, gyrB, and nodC gene sequences, and seven of them had their symbiotic efficiency assessed. Thirty-two strains were obtained and 22 of them were nodC/nifH positive. Twenty strains clustered within Paraburkholderia and two within Rhizobium by 16S rRNA gene sequencing. The beta-rhizobia were similar to P. phenoliruptrix (12) and P. diazotrophica (8). Both alpha-rhizobia were closely related to R. miluonense. The recA + gyrB phylogenetic analysis clustered four and five strains within the P. phenoliruptrix and P. diazotrophica branches, respectively, but they were somewhat divergent to the 16S rRNA phylogeny. For Rhizobium sp. ESA 637, the recA + gyrB phylogeny clustered the strain with R. jaguaris. The nodC phylogeny indicated that ESA 626, ESA 629, and ESA 630 probably represented a new symbiovar branch. The inoculation assay showed high symb... Mostrar Tudo |
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Palavras-Chave: |
Bioma Caatinga; Fixação biológica de nitrogênio; Região semiárida brasileira; Rizobio. |
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Thesagro: |
Caatinga; Floresta Tropical; Inoculante; Solo. |
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Thesaurus Nal: |
Soil. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02903naa a2200361 a 4500
001 2133773
005 2021-08-20
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.syapm.2021.126208$2DOI
100 1 $aDIAS, M. A. M.
245 $aParaburkholderia spp. are the main rhizobial microsymbionts of Mimosa tenuiflora (Willd.) Poir. in soils of the Brazilian tropical dry forests (Caatinga biome).$h[electronic resource]
260 $c2021
520 $aMimosa tenuiflora (Willd.) Poir. is widespread in southern and central American drylands, but little information is available concerning its associated rhizobia. Therefore, this study aimed to characterize M. tenuiflora rhizobia from soils of the tropical dry forests (Caatinga) in Pernambuco State, Brazil, at the molecular and symbiotic levels. Soil samples of pristine Caatinga areas in four municipalities were used to grow M. tenuiflora. First, the bacteria from root nodules were subjected to nodC/nifH gene amplification, and the bacteria positive for both genes had the 16S rRNA gene sequenced. Then, ten strains were evaluated using recA, gyrB, and nodC gene sequences, and seven of them had their symbiotic efficiency assessed. Thirty-two strains were obtained and 22 of them were nodC/nifH positive. Twenty strains clustered within Paraburkholderia and two within Rhizobium by 16S rRNA gene sequencing. The beta-rhizobia were similar to P. phenoliruptrix (12) and P. diazotrophica (8). Both alpha-rhizobia were closely related to R. miluonense. The recA + gyrB phylogenetic analysis clustered four and five strains within the P. phenoliruptrix and P. diazotrophica branches, respectively, but they were somewhat divergent to the 16S rRNA phylogeny. For Rhizobium sp. ESA 637, the recA + gyrB phylogeny clustered the strain with R. jaguaris. The nodC phylogeny indicated that ESA 626, ESA 629, and ESA 630 probably represented a new symbiovar branch. The inoculation assay showed high symbiotic efficiency for all tested strains. The results indicated high genetic diversity and efficiency of M. tenuiflora rhizobia in Brazilian drylands and included P. phenoliruptrix-like bacteria in the list of efficient beta-rhizobia in the Caatinga biome.
650 $aSoil
650 $aCaatinga
650 $aFloresta Tropical
650 $aInoculante
650 $aSolo
653 $aBioma Caatinga
653 $aFixação biológica de nitrogênio
653 $aRegião semiárida brasileira
653 $aRizobio
700 1 $aBOMFIM, C. S. G.
700 1 $aRODRIGUES, D. R.
700 1 $aSILVA, A. F. da
700 1 $aSANTOS, J. C. S.
700 1 $aNASCIMENTO, T. R. do
700 1 $aMARTINS, L. M. V.
700 1 $aDANTAS, B. F.
700 1 $aRIBEIRO, P. R. de A.
700 1 $aFREITAS, A. D. S. de
700 1 $aFERNANDES JUNIOR, P. I.
773 $tSystematic and Applied Microbiology$gv. 44, 2021.
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Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 9 | |
| 2. |  | GARCIA, M. B.; MENDES, I. V.; SANTANA, R. H.; KRUGER, R. H.; GLADDEN, J.; QUIRINO, B. F. Diversidade microbiana e degradação de lignina em cultura enriquecida. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 42.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 3. | | COLOMBO, G. S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R.; QUIRINO, B. F. Functional expression of a new xylose isomerase in Saccharomyces Cerevisiae. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió, Anais... Maceió: SBM, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | MENDES, I. V.; GARCIA, M. B.; SANTANA, R. H.; GLADDEN, J.; KRÜGER, R. H.; QUIRINO, B. F. Análise funcional e da diversidade de um consórcio microbiano capaz de degradar lignina. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 36.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | COLOMBO, G. de S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Análise filogenética e expressão heteróloga de uma xilose isomerase bacteriana em Saccharomyces cerevisiae. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 101-107 il.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | COLOMBO, G. de S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Descoberta de novos genes de xilose isomerase em rúmen de cabras brasileiras. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 16-21.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | COLOMBO, G. de S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Expressão funcional de uma nova xilose isomerase em Saccharomyces cerevisiae. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 50.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. Bacterial diversity dynamics in microbial consortia selected for lignin utilization. PLoS ONE, v. 16, n. 9, e0255083, 2021. PDF: il. color.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | COLOMBO, G. DE S.; MENDES, I. V.; SOUTO, B. de M.; BARRETO, C. C.; SERRA, L. A.; NORONA, E. F.; PARACHIN, N. S.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Identification and functional expression of a new xylose isomerase from the goat rumen microbiome in Saccharomyces cerevisiae. Letters in Applied Microbiology, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 9 | |
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