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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
28/11/1994 |
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Data da última atualização: |
26/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHÃES, B. P.; WAYNE, R.; HUMBER, R. A.; SHIELDS, E. J.; ROBERTS, D. W. |
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Afiliação: |
BONIFACIO PEIXOTO MAGALHAES, CNPAF. |
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Título: |
Calcium-regulated appressorium formation of the entomopathogenic fungus Zoophthora radicans. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Protoplasma, v. 160, p. 77-88, June 1991. |
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DOI: |
https://doi.org/10.1007/BF01539959 |
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Idioma: |
Inglês |
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Conteúdo: |
The fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to be formulated. MenosThe fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to b... Mostrar Tudo |
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Palavras-Chave: |
Appressorium formation; Entomopathogen. |
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Thesagro: |
Fungo; Fungo Entomógeno; Zoophthora Radicans. |
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Thesaurus Nal: |
Calcium; Calmodulin; Entomopathogenic fungi. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02322naa a2200277 a 4500
001 1198109
005 2021-10-26
008 1991 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/BF01539959$2DOI
100 1 $aMAGALHÃES, B. P.
245 $aCalcium-regulated appressorium formation of the entomopathogenic fungus Zoophthora radicans.$h[electronic resource]
260 $c1991
520 $aThe fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to be formulated.
650 $aCalcium
650 $aCalmodulin
650 $aEntomopathogenic fungi
650 $aFungo
650 $aFungo Entomógeno
650 $aZoophthora Radicans
653 $aAppressorium formation
653 $aEntomopathogen
700 1 $aWAYNE, R.
700 1 $aHUMBER, R. A.
700 1 $aSHIELDS, E. J.
700 1 $aROBERTS, D. W.
773 $tProtoplasma$gv. 160, p. 77-88, June 1991.
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Embrapa Arroz e Feijão (CNPAF) |
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| 3. |  | SILVA JÚNIOR, J. F.; BEZERRA, J. E. F.; TAVARES, J. A.; LEDERMAN, I. E.; MELO NETO, M. L. de. Caracterización y evaluación de germoplasma de guayabo (psidium guajava l.) en la región semiárida del estado de Pernambuco, Brasil. Revista Caatinga, Mossoró, v.21, n.3, p. 94 - 999, jul./set. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 4. | | PEDROSA, A. C.; GONZAGA NETO, L.; MELO NETO, M. L. de; BEZERRA, J. M. F.; LEDERMAN, I. E. Introducao, avaliacao e caracterizacao de selecoes de goiabeiras (Psidium guajava L.) na chapada do Araripe-Pernambuco: II - caracteristicas produtivas. Revista Brasileira de Fruticultura, v. 14, n. 2, p. 95-100, 1992.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| 8. | | MELO NETO, M. L. de; GONZAGA NETO, L.; BEZERRA, J. E. F.; LEDERMAN, I.; ASSIS, J. S. de. Efeito de indutor de brotação associado a desfolha química e manual na indução floral de pinheiras (Annona squamosa L.). In: CONGRESSO BRASILEIRO DE FRUTICULTURA., 17., 2002, Belém, PA. Anais... Belém, PA: SBF, 2002. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 9. | | MELO NETO, M. L. de; GONZAGA NETO, L.; BEZERRA, J. E. F.; LEDERMAN, I.; ASSIS, J. S. de. Efeito da poda e da cianamida hidrogenada associados à desfolha química e manual na indução floral de pinheiras. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 10.; CONGRESSO LATINO AMERICANO DE FISIOLOGIA VEGETAL, 12., 2005, Recife. Anais... Recife: SBFV, 2005. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 10. | | BEZERRA, J. E. F; LEDERMAN, I. E.; PEDROSA, A. C.; DANTAS, A. P.; GONZAGA NETO, L.; PEREIRA, R. de C. A.; MELO NETO, M. L. de. Coleta e preservacao de especies frutiferas tropicais nativas e exoticas em Pernambuco. In: SIMPOSIO LATINO-AMERICANO SOBRE RECURSOS GENETICOS DE ESPECIES HORTICOLAS, 1., 1989, Campinas. Anais... Campinas: Fundacao Cargill, 1990. p. 140-147.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 11. | | SILVA JÚNIOR, J. F. da; BEZERRA, J. E. F.; TAVARES, J. A.; LEDERMAN, I. E.; MELO NETO, M. L. de; GONZAGA NETO, L. Caracterização e avaliação de germoplasma de goiaba (Psidium guajava L.) no semi-árido do Estado de Pernambuco, Brasil. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA E CARIBE, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM ARACHIS, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM RECURSOS GENÉTICOS FLORESTAIS, 3., 2001, Londrina. Anais... Londrina: IAPAR, 2001. p. 421-423.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 11 | |
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