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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
23/05/2023 |
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Autoria: |
MAYER, D. T.; SQUIERS, C. D.; BOGART, R.; OLOUFA, M. M. |
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Título: |
The technique for characterizing mammalian spermatozoa as dead or living by differential staining. |
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Ano de publicação: |
1951 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 10, n. 1, p. 226-235, 1951. |
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DOI: |
10.2527/jas1951.101226x. |
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Idioma: |
Inglês |
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Conteúdo: |
A staining technique has been described which separates mammalian spermatozoa into two groups; viz., stained or dead cells and unstained or presumably live cells. The differential staining technique has been shown to be applicable to human, ram, bull, rabbit, and stallion spermatozoa. A substance present in the semen plasma of the boar interferes with the differential staining of the spermatozoa of this species. The water-soluble halogen derivatives of fluorescein were the only dyes studied capable of differentially staining mammalian spermatozoa. A new background stain, fast green FCF has been investigated and found superior to opal blue for this purpose. Studies with the staining principle have shown that differential staining is dependent upon the presence of ionizable substances and that the pH must be held within a narrow range for optimal staining results. |
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Palavras-Chave: |
Andrologia; Coloring agents; Labeling. |
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Thesagro: |
Bovino; Caprino; Ovino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Cattle; Goats; Sheep; Spermatozoa; Staining. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01734naa a2200325 a 4500
001 1519970
005 2023-05-23
008 1951 bl uuuu u00u1 u #d
024 7 $a10.2527/jas1951.101226x.$2DOI
100 1 $aMAYER, D. T.
245 $aThe technique for characterizing mammalian spermatozoa as dead or living by differential staining.$h[electronic resource]
260 $c1951
520 $aA staining technique has been described which separates mammalian spermatozoa into two groups; viz., stained or dead cells and unstained or presumably live cells. The differential staining technique has been shown to be applicable to human, ram, bull, rabbit, and stallion spermatozoa. A substance present in the semen plasma of the boar interferes with the differential staining of the spermatozoa of this species. The water-soluble halogen derivatives of fluorescein were the only dyes studied capable of differentially staining mammalian spermatozoa. A new background stain, fast green FCF has been investigated and found superior to opal blue for this purpose. Studies with the staining principle have shown that differential staining is dependent upon the presence of ionizable substances and that the pH must be held within a narrow range for optimal staining results.
650 $aCattle
650 $aGoats
650 $aSheep
650 $aSpermatozoa
650 $aStaining
650 $aBovino
650 $aCaprino
650 $aOvino
650 $aReprodução
650 $aSêmen
653 $aAndrologia
653 $aColoring agents
653 $aLabeling
700 1 $aSQUIERS, C. D.
700 1 $aBOGART, R.
700 1 $aOLOUFA, M. M.
773 $tJournal of Animal Science$gv. 10, n. 1, p. 226-235, 1951.
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