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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
31/05/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORA, M. A. E.; CHACÓN-OROZCO, J. G.; HARAKAVA, R.; ROUWS, J. R. C.; FRAGA, M. E. |
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Afiliação: |
MARGY A. ESPARZA MORA, UFRRJ; JULIO G. CHACÓN-OROZCO, INSTITUTO BIOLÓGICO DE SÃO PAULO; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO DE SÃO PAULO; JANAINA RIBEIRO COSTA ROUWS, CNPAB; MARCELO E. FRAGA, UFRRJ. |
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Título: |
Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
African Journal of Microbiology Research, Nairobi, v. 10, n. 19, p. 662-668, may, 2016 |
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ISSN: |
1996-0808 |
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DOI: |
10.5897/AJMR2016.7973 |
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Idioma: |
Inglês |
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Conteúdo: |
Entomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations: 1×106, 1×107, 1×108 and 1×109 conidia.ml-1 were in vitro evaluated and the lethal concentrations (LC50 and LC90) as lethal time (LT50 and LT90) were determined. Sequencing of rDNA-ITS 500 bp fragments allowed the identification of Ma10MI, Ma12MI, Ma58MI and Ma11MI isolates as M. anisopliae and Bb11MI, Bb79MI, Bb53MI and Bb27MI isolates as B. bassiana by sequence comparison to GenBank. According to the pathogenicity test, B. bassiana strains with higher performance for G. mellonella were Bb53MI and Bb79MI with LC50= 3.98×107 and 1.04×107 conidia.ml-1 and LT50= 5.46 and 5.27 days, respectively, as well as to T. molitor was Bb79MI with LC50= 1.03×107 conidia.ml-1 and LT50= 5.57 days. The M. anisopliae strains with the best performance were Ma58MI and Ma10MI both T. molitor (LC50= 1.0×106 and 1.0×107 conidia.ml-1 and LT50= 4.06 and 5.22 days, respectively) and G. mellonella (LC50= 6.3×107 and 1.1×108 conidia.ml-1 and LT50= 5.18 and 6.37 days, respectively), these isolates might be considered as new promising candidates for the microbial pest control. |
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Palavras-Chave: |
Entomopathogenic funghi; Microbial control; Molecular taxonomy. |
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Thesaurus Nal: |
Pathogenicity. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02321naa a2200241 a 4500
001 2045933
005 2016-05-31
008 2016 bl uuuu u00u1 u #d
022 $a1996-0808
024 7 $a10.5897/AJMR2016.7973$2DOI
100 1 $aMORA, M. A. E.
245 $aMolecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae mellonella (Lepidoptera$bPyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae.
260 $c2016
520 $aEntomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations: 1×106, 1×107, 1×108 and 1×109 conidia.ml-1 were in vitro evaluated and the lethal concentrations (LC50 and LC90) as lethal time (LT50 and LT90) were determined. Sequencing of rDNA-ITS 500 bp fragments allowed the identification of Ma10MI, Ma12MI, Ma58MI and Ma11MI isolates as M. anisopliae and Bb11MI, Bb79MI, Bb53MI and Bb27MI isolates as B. bassiana by sequence comparison to GenBank. According to the pathogenicity test, B. bassiana strains with higher performance for G. mellonella were Bb53MI and Bb79MI with LC50= 3.98×107 and 1.04×107 conidia.ml-1 and LT50= 5.46 and 5.27 days, respectively, as well as to T. molitor was Bb79MI with LC50= 1.03×107 conidia.ml-1 and LT50= 5.57 days. The M. anisopliae strains with the best performance were Ma58MI and Ma10MI both T. molitor (LC50= 1.0×106 and 1.0×107 conidia.ml-1 and LT50= 4.06 and 5.22 days, respectively) and G. mellonella (LC50= 6.3×107 and 1.1×108 conidia.ml-1 and LT50= 5.18 and 6.37 days, respectively), these isolates might be considered as new promising candidates for the microbial pest control.
650 $aPathogenicity
653 $aEntomopathogenic funghi
653 $aMicrobial control
653 $aMolecular taxonomy
700 1 $aCHACÓN-OROZCO, J. G.
700 1 $aHARAKAVA, R.
700 1 $aROUWS, J. R. C.
700 1 $aFRAGA, M. E.
773 $tAfrican Journal of Microbiology Research, Nairobi$gv. 10, n. 19, p. 662-668, may, 2016
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| 20. |  | BENDAHAN, A. B.; MEDEIROS, R. D. de; VILARINHO, A. A. Bioma Amazônia. In: BALBINO, L. C.; MARTINEZ, G. B.; GALERANI, P. R. (Ed.). Ações de transferência de tecnologia de sistemas de integração lavoura-pecuária-floresta 2007-2011. Brasília, DF: Embrapa; Belém, PA: Embrapa Amazônia Oriental; Planaltina, DF: Embrapa Cerrados, 2011.| Tipo: Capítulo em Livro Técnico-Científico |
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