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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AGUIAR-PERECIN, M. L. R.; MONDIN, M.; SANTOS-SEREJO, J. A. dos; VIDA, A. C. F.; MOLINA, S. C. M. |
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Afiliação: |
Margarida L. R. Aguiar-Perecin, USP; Mateus Mondin, USP; Janay Almeida dos Santos-Serejo, CNPMF; Ana C. F. Vida, USP; Silvia C. M. Molina, USP. |
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Título: |
Analysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088 |
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ISSN: |
0967-3849 |
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Idioma: |
Inglês |
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Notas: |
Edição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz. DOI: 10.1007/s10577 ISSN 0967-3849 em papel |
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Conteúdo: |
The analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratios) of chromosomes 2 and 4 formed normal bivalents at pachtytene. This aspect apparently provides evidence for epigenetic location of centromeres, a finding that has been observed in many organisms. MenosThe analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratio... Mostrar Tudo |
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Palavras-Chave: |
Maize cytogenetics. |
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Thesaurus Nal: |
chromosomes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02599nam a2200205 a 4500
001 1654675
005 2023-07-24
008 2007 bl uuuu u00u1 u #d
022 $a0967-3849
100 1 $aAGUIAR-PERECIN, M. L. R.
245 $aAnalysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes.$h[electronic resource]
260 $aIn: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088$c2007
500 $aEdição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz. DOI: 10.1007/s10577 ISSN 0967-3849 em papel
520 $aThe analysis of pachytene chromosomes has greatly contributed to maize cytogenetics. Recently, FISH procedures using repeated DNA sequences have been described and improved the identification of somatic chromosomes. Due to the polymorphism of knobs and repetitive DNA sequences, karyotype diversity is observed among varieties. Mitotic chromosome analysis is valuable for the screening of several individuals, and studies of evolution. We investigaded root-tip chromosomes of inbred lines and hybrids, derived from a tropical variety (Cateto races), using the following sequences as probes: subtelomeric 4-12-1 clone (PNAS 101, 13555), knob 180-bp sequence, 5S rDNA, centromeric satellite 4 (Cent 4). Pachytene chromosomes and C-banded mitotic metaphases were previously analysed, and except for chromosomes 2, 4 and 5, most chromosomes were distinguished by their length, arm ratio or presence of knobs. We identified chromosomes 2 and 4 using probes of 5S rDNA (2L) and Cent4, respectively, but their arm ratios were atypical. Signals of the 180-bp sequence were correspondent to knobs detected by C-banding, and additional small signals were found at 1S, 6S and 9S, located at arm tips, a feature also found in other varieties. The subtelomeric sequence was observed at 1S, 2S, 4SL, 5S, 8L. One line was crossed with KYS, an inbred extensively investigated, and the following observations shoul be emphasized: differences in the subtelomeric sequence signals; homologues (with different arm ratios) of chromosomes 2 and 4 formed normal bivalents at pachtytene. This aspect apparently provides evidence for epigenetic location of centromeres, a finding that has been observed in many organisms.
650 $achromosomes
653 $aMaize cytogenetics
700 1 $aMONDIN, M.
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aVIDA, A. C. F.
700 1 $aMOLINA, S. C. M.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 2. |  | MARTINS, A. G. G. M.; SIHLER, W.; DIAS, S. C.; SOUZA, M. L. Construção de baculovírus recombinante para expressão de inibidor alpha-amilase BIII isolado de centeio. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 54.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | BRAGANÇA, S. M.; SILVA, E. B.; MARTINS, A. G.; SANTOS, L. P.; LANI, J. A.; VOLPI, P. S. Resposta do cafeeiro Conilon à adubação de NPK em sistema de plantio adensado. Coffee Science, Lavras, v. 4, n. 1, p. 67-75, jan./jun. 2009| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 6. | | SIHLER, W.; SISSON, D.; MARTINS, A. G. M.; DIAS, S. C.; FONTES, E. M. G.; SOUZA, M. L. de. Construction of recombinant baculovirus expressing the protein inhibitor alpha-amilase BIII isolated from rye seeds. Virus Reviews & Research, v. 11, suppl. 1, p. 111, Nov. 2006. Edição dos Resumos do 17 Encontro Nacional de Virologia, Campos do Jordão, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | MESQUITA, A. L. M.; GUIMARAES, J. A.; CÔNSOLI, R. C. M.; BRAGA SOBRINHO, R.; MARTINS, A. G. Espécies de tripes (Thysanoptera, Thripidae) associadas à plantas cultivadas e silvestres no Nordeste do Brasil. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Resumos... Natal: Sociedade Entomológica do Brasil, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 9. | | MESQUITA, A. L. M.; GUIMARAES, J. A.; CÔNSOLI, R. C. M.; BRAGA SOBRINHO, R.; MARTINS, A. G. Espécies de tripes (Thysanoptera, Thripidae) associados à plantas cultivadas e silvestres no nordeste do Brasil. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal, RN. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | ALVES, R. E.; FILGUEIRAS, H. A. C.; MENEZES, J. B.; ASSIS, J. S. de; LIMA, M. A. C. de; AMORIM, T. B. F.; MARTINS, A. G. Colheita e pós-colheita. In: GENU, P. J. de C.; PINTO, A. C. de Q. (Ed.). A cultura da mangueira. Brasília, DF: Embrapa Informação Tecnológica, 2002. cap. 17, p. 381-405. il.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| 11. | | SOUZA, M. F.; TONON, J. M.; DOCEMA, L. I.; MARTINS, A. G. B.; SILVA, T. M.; MORICONI, W.; VIEIRA, H. B.; CARVALHO, C. G. P.; RAMOS, N. P. Desempenho agronômico de genótipos de girassol na safra 2019/2020, no município de Espírito Santo do Pinhal-SP. Sunflower productive performance in the 2019/2020 wet season at Espírito Santo do Pinhal-SP. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 23.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 11., 2020, Londrina, PR. Anais... Londrina: Embrapa Soja, 2020. p. 129-133 (Embrapa Soja. Documentos, 432).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Soja. |
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| 12. | | MUNIZ, M. F. S.; SILVA, E. J.; CASTRO, J. M. da C. e; ROCHA, F. S.; ALENCAR, L. M. C.; MARTINS, A. G. Etiologia da casca preta do inbame no Estado de Alagoas. In: INTERNATIONAL CONGRESS OF TROPICAL NEMATOLOGY, 2., 2009, Maceió. Abstracts... Maceió: ONTA: SBN, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 13. | | VERDIN FILHO, A. C.; FERRÃO, R. G.; FERRAO, M. A. G.; SILVEIRA, J. S. M.; VOLPI, P. S.; FONSECA, A. F. A. da; LANI, J. A.; MARTINS, A. G.; FERRÃO, L. F. V.; SILVEIRA, T. B. Poda programada de ciclo para o café conilon. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Inovação científica, competitividade e mudanças climáticas: anais... Vitória: Consórcio Pesquisa Café, 2009.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 13 | |
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