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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
11/08/2023 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, E. L. dos; BESPALHOK FILHO, J. C.; HANSEL, F. A.; SOUSA, J. A. T.; AUER, C. G.; STEINER, N.; DEGENHARDT, J. |
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Afiliação: |
EVELYN LOPES DOS SANTOS, Federal University of Paraná; JOÃO CARLOS BESPALHOK FILHO, Federal University of Paraná; FABRICIO AUGUSTO HANSEL, CNPF; JOÃO ARTHUR TIKLER SOUSA, Federal University of Paraná; CELSO GARCIA AUER, CNPF; NEUSA STEINER, Federal University of Santa Catarina; JULIANA DEGENHARDT GOLDBACH, CNPF. |
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Título: |
Azacytidine affects gene expression and metabolic profile of Pinus elliottii x Pinus caribaea var. hondurensis embryogenic cell lines. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 155, p. 637-651, 2023. |
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DOI: |
https://doi.org/10.1007/s11240-023-02584-6 |
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Idioma: |
Português |
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Conteúdo: |
The current study evaluated the effect of (i) genotypes on somatic embryogenesis initiation of Pinus elliottii x Pinus caribaea var. hondurensis and (ii) 5-azacytidine (5-azaC), a potential agent for embryogenic competence reacquisition. For the establishment of embryogenic cell lines (ECLs), immature megagametophytes of thirteen families were cultured on an initiation medium. After 17 months, two aged ECLs (ECL02 and ECL05) were exposed for 10 days to 5-azaC (0, 50 and 150 ?M). Gene expression analysis of Somatic Embryogenesis Receptor Kinase 1 (SERK1) and Leafy Cotyledon 1 (LEC1) were carried out by RT-qPCR in both ECLs. Metabolomic analysis via GC?MS were performed in the aged ECL05 and in a young ECL. ECLs initiation was genotype-dependent. The aged ECL05 exposed to 150 µM 5-azaC showed an increase in SERK1 and LEC1 expressions in comparison to the control and a similar metabolic profile to the young ECL. It suggests that 5-azaC has an effect on embryogenic morphology of the aged ECL05. On the other hand, it seems that the response to 5-azaC is also affected by the genotype, as the expression of both genes showed an opposite pattern in the ECL02. |
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Palavras-Chave: |
Embryogenic potential; Epigenética. |
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Thesagro: |
Biotecnologia; Embriogénese; Pinus Caribaea; Pinus Elliottii. |
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Thesaurus Nal: |
Biotechnology; DNA methylation; Epigenetics; Somatic embryos. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02181naa a2200325 a 4500
001 2155864
005 2024-02-15
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-023-02584-6$2DOI
100 1 $aSANTOS, E. L. dos
245 $aAzacytidine affects gene expression and metabolic profile of Pinus elliottii x Pinus caribaea var. hondurensis embryogenic cell lines.$h[electronic resource]
260 $c2023
520 $aThe current study evaluated the effect of (i) genotypes on somatic embryogenesis initiation of Pinus elliottii x Pinus caribaea var. hondurensis and (ii) 5-azacytidine (5-azaC), a potential agent for embryogenic competence reacquisition. For the establishment of embryogenic cell lines (ECLs), immature megagametophytes of thirteen families were cultured on an initiation medium. After 17 months, two aged ECLs (ECL02 and ECL05) were exposed for 10 days to 5-azaC (0, 50 and 150 ?M). Gene expression analysis of Somatic Embryogenesis Receptor Kinase 1 (SERK1) and Leafy Cotyledon 1 (LEC1) were carried out by RT-qPCR in both ECLs. Metabolomic analysis via GC?MS were performed in the aged ECL05 and in a young ECL. ECLs initiation was genotype-dependent. The aged ECL05 exposed to 150 µM 5-azaC showed an increase in SERK1 and LEC1 expressions in comparison to the control and a similar metabolic profile to the young ECL. It suggests that 5-azaC has an effect on embryogenic morphology of the aged ECL05. On the other hand, it seems that the response to 5-azaC is also affected by the genotype, as the expression of both genes showed an opposite pattern in the ECL02.
650 $aBiotechnology
650 $aDNA methylation
650 $aEpigenetics
650 $aSomatic embryos
650 $aBiotecnologia
650 $aEmbriogénese
650 $aPinus Caribaea
650 $aPinus Elliottii
653 $aEmbryogenic potential
653 $aEpigenética
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aHANSEL, F. A.
700 1 $aSOUSA, J. A. T.
700 1 $aAUER, C. G.
700 1 $aSTEINER, N.
700 1 $aDEGENHARDT, J.
773 $tPlant Cell, Tissue and Organ Culture$gv. 155, p. 637-651, 2023.
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Registro original: |
Embrapa Florestas (CNPF) |
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| Registros recuperados : 7 | |
| 2. |  | LANGE, A.; MARTINES, A. M.; SILVA, M. A. C. da; SORREANO, M. C. M.; CABRAL, C. P.; MALAVOLTA, E. Efeito de deficiência de micronutrientes no estado nutricional da mamoneira cultivar Iris. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 1, p. 61-67, jan. 2005 Título em inglês: Micronutrient deficiency effect on the nutritional status of the castor bean cultivar Iris.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | NAKATANI, A. S.; MARTINES, A. M.; NOGUEIRA, M. A.; FAGOTTI, D. S. L.; OLIVEIRA, A. G.; BINI, D.; SOUSA, J. P.; CARDOSO, E. J. B. N. Changes in the genetic structure of Bacteria and microbial activity in an agricultural soil amended with tannery sludge. Soil Biology & Biochemistry, v. 43, n. 1, p. 106-114, Jan. 2011.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | NAKATANI, A. S.; NOGUEIRA, M. A.; MARTINES, A. M.; SANTOS, C. A. dos; BALDESIN, L. F.; MARSCHNER, P.; CARDOSO, E. J. B. N. Effects of tannery sludge application on physiological and fatty acid profiles of the soil microbial community. Applied Soil Ecology, v. 61, p. 92-99, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | BATISTA JÚNIOR, C. B.; ALBINO, U. B.; MARTINES, A. M.; SARIDAKIS, D. P.; MATSUMOTO, L. S.; AVANZI, M. A.; ANDRADE, G. Efeito fungistático de Bacillus thuringiensis e de outras bactérias sobre alguns fungos fitopatogênicos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 8, p. 1189-1194, ago. 2002 Notas Científicas. Título em inglês: Fungistatic effect of Bacillus thuringiensis and of other bacteria on some plant pathogenic fungi.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | SANTOS, C. A.; PANCHONI, L. C.; BINI, D.; KUWANO, B. H.; CARMO, K. B.; SILVA, S. M. C. P.; MARTINES, A. M.; ANDRADE, G.; ANDRADE, D. S.; CARDOSO, E. J. B. N.; ZANGARO, W.; NOGUEIRA, M. A. Land application of municipal landfill leachate: fate of ions and ammonia volatilization. Journal of Environmental Quality, v. 42, n. 2, p. 523-531, Mar./Apr. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | NIEMEYER, J. C.; CARDOSO, E. J. B. N.; NUNES, M. E. T.; ALVES, P. R. L.; MARTINES, A. M.; ANDRÉA, M. M. de; BROWN, G. G.; NIVA, C. C. Preparo do substrato teste. In: NIVA, C. C.; BROWN, G. G. (Ed.). Ecotoxicologia terrestre: métodos e aplicações dos ensaios com oligoquetas. Brasília, DF: Embrapa, 2019. Cap. 7, p. 143-151.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Florestas. |
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| Registros recuperados : 7 | |
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