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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/02/2016 |
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Data da última atualização: |
26/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FANÉ, R.; TRAORÉ, D.; KASSOGUÉ, A.; SAMALÉ, F.; DICKO, A. H.; HAMADOUN, A.; VALICENTE, F. H.; BABANA, A. H. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Genetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
British Biotechnology Journal, v. 12, n. 1, p. 1-8, 2016. |
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DOI: |
10.9734/BBJ/2016/20432 |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only. MenosAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of o... Mostrar Tudo |
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Palavras-Chave: |
Helicoverpa armegira. |
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Thesagro: |
Bacillus thuringiensis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02685naa a2200241 a 4500
001 2036996
005 2017-09-26
008 2016 bl uuuu u00u1 u #d
024 7 $a10.9734/BBJ/2016/20432$2DOI
100 1 $aFANÉ, R.
245 $aGenetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile.$h[electronic resource]
260 $c2016
520 $aAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only.
650 $aBacillus thuringiensis
653 $aHelicoverpa armegira
700 1 $aTRAORÉ, D.
700 1 $aKASSOGUÉ, A.
700 1 $aSAMALÉ, F.
700 1 $aDICKO, A. H.
700 1 $aHAMADOUN, A.
700 1 $aVALICENTE, F. H.
700 1 $aBABANA, A. H.
773 $tBritish Biotechnology Journal$gv. 12, n. 1, p. 1-8, 2016.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 17 | |
| 4. |  | PANCIO, S.; NUNES, A. M.; GONÇALVES, R. S.; LISBOA, H.; MANICA-BERTO, R.; GARCIA, M. S.; NAVA, D. E. Biology of Doryctobracon brasiliensis at different temperatures: development of life table and determining thermal requirements. Journal of Applied Entomology, v. 140, p. 775-785, 2016. http://onlinelibrary.wiley.com/doi/10.1111/jen.12308/pdf| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | SCHEUNEMANN, T.; GONÇALVES, R. da S.; NÖRNBERG, S. D.; MANICA-BERTO, R.; BOTTON, M.; GRÜTZMACHER, A. D.; NAVA, D. E. Efeito de iscas tóxicas sobre o parasitoide Diachasmimorpha longicaudata (Hymenoptera: Braconidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia, GO. Resumos... Goiânia: Embrapa Arroz e Feijão; Universidade Federal de Goiás, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | SCHEUNEMANN, T.; GONÇALVES, R. da S.; NÖRNBERG, S. D.; MANICA-BERTO, R.; BOTTON, M.; GRÜTZMACHER, A. D.; NAVA, D. E. Efeito de iscas tóxicas sobre o parasitoide Diachasmimorpha longicaudata (Hymenoptera: Braconidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia, GO. Resumos... Goiânia: Embrapa Arroz e Feijão; Universidade Federal de Goiás, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | ONGARATTO, S; PINTO, K.; MANICA‑BERTO, R.; GONÇALVES, R. da S.; NÖRNBERG, S. D.; BERNARDI, D.; NAVA, D. E. Different Protein Sources of Larval Diet on the Rearing of Anastrepha fraterculus (Diptera: Tephritidae): Biological and Nutritional Analyses. Neotropical Entomology, v. 53, p. 1031–1044, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | NEGRETTI, R. R. D.; GOMES, C. B.; MANICA-BERTO, R.; AGOSTINETTO, D.; SOMAVILLA, L.; MEDINA, I. L.; SCHEUERMANN, K. K. Ocorrência e caracterização de espécies do nematóide Das galhas (Meloidogyne spp.) em arroz irrigado no sul do Brasil. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 7., 2011, Balneário Camboriú. Racionalizando recursos e ampliando oportunidades: anais. Itajaí: Epagri, 2011. p. 555-558.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | MANICA-BERTO, R.; PEGORARO, C.; MISTURA, C. C.; BRESOLIN, A. P. S.; RUFATO, A. De R.; FACHINELLO, J. C. Similaridade genética entre cultivares de marmeleiro avaliadas por marcadores AFLP. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 5, p. 568-571, maio 2013. Notas científicas. Título em português: Genetic similarity between quince cultivars evaluated by AFLP markers.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | GONÇALVES, R. S.; ANDREAZZA, F.; LISBÔA, H.; GRÜTZMACHER, A. D.; VALGAS, R. A.; MANICA-BERTO, R.; NÖRNBERG, S. D.; NAVA, D. E. Basis for the Development of a Rearing Technique of Aganaspis pelleranoi (Hymenoptera: Figitidae) in Anastrepha fraterculus (Tephritidae: Diptera). Journal of Economic Entomology, v. 109, n. 3, p. 1094-1101, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 12. | | PICOLOTTO, L.; MANICA-BERTO, R.; PAZIN, D.; PASA, M. da S.; SCHMITZ, J. D.; PREZOTTO, M. E.; BETEMPS, D.; BIANCHI, V. J.; FACHINELLO, J. C. Características vegetativas, fenológicas e produtivas do pessegueiro cultivar Chimarrita enxertado em diferentes porta-enxertos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 6, p. 583-589, jun. 2009. Título em inglês: Productivity and physicochemical composition of berries of grapevine cultivars grafted onto different rootstocks.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | GOMES, C. B.; NEGRETTI, R. R. D.; MATTOS, V. S.; SOMAVILLA, L.; MANICA-BERTO, R.; AGOSTINETTO, D.; CARNEIRO, R. M. D. G. Characterization of meloidogyne species from irrigated rice in southern Brazil. Journal of Nematology, v. 46, n. 2, p. 168-169, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | PINTO, C. C.; GRÜTZMACHER, A. D.; ROSA, A. P. S. A. da; MANICA-BERTO, R.; MENDES, S. M.; ARGE, L. W. P.; BORGES, C. T. Diversidade molecular entre populações de Spodoptera frugiperda no Brasil avaliada por marcadores AFLP. Pesquisa Agropecuária Brasileira, Brasília, v. 50, n. 4, p. 343-346, abr. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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| 15. | | ZANATTA, J. F.; PROCOPIO, S. D. O.; MANICA-BERTO, R.; PAULETTO, E. A.; CARGNELLUTI FILHO, A.; VARGAS, L.; SGANZERLA, D. C.; ROSENTHAL, M. D.; PINTO, J. J. O. Teores de água no solo e eficácia do herbicida fomesafen no controle de amaranthus hybridus. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios: anais. Fortaleza: SBCS, 2009. 1 CD-ROM. Física do solo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 16. | | ZANATTA, J. F.; PROCOPIO, S. de O.; MANICA-BERTO, R.; PAULETTO, E. A.; CARGNELUTTI FILHO, A.; VARGAS, L.; SGANZERLA, D. C.; ROSENTHAL, M. D'. A.; PINTO, J. J. O. Teores de água no solo e eficácia do herbicida fomesafen no controle de Amaranthus hybridus. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios: anais. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. 5 p. 1 CD-ROM. Física do solo: trabalho 876.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Trigo. |
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| 17. | | ZANATTA, J. F.; PROCOPIO, S. de O.; MANICA-BERTO, R.; PAULETTO, E. A.; CARGNELUTTI FILHO, A.; VARGAS, L.; SGANZERLA, D. C.; ROSENTHAL, M. D'. A.; PINTO, J. J. O. Teores de água no solo e eficácia do herbicida glyphosate no controle de Euphorbia heterophylla. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios: anais. [Viçosa, MG]: SBCS; Fortaleza: UFC, 2009. 5 p. 1 CD-ROM. Biologia do solo: trabalho 877.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 17 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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