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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; COSENTINO, I. O.; PINTO, P. H. N.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 30, n. 9, p. 1234-1244, 2018. |
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DOI: |
https://doi.org/10.1071/rd17337 |
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Idioma: |
Inglês |
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Conteúdo: |
The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes. MenosThe present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality... Mostrar Tudo |
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Palavras-Chave: |
Oócito. |
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Thesagro: |
Biologia Molecular; Ovelha. |
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Thesaurus Nal: |
Molecular biology; Oocytes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02470naa a2200301 a 4500
001 2102508
005 2022-08-09
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/rd17337$2DOI
100 1 $aBRAGANÇA, G. M.
245 $aDose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes.$h[electronic resource]
260 $c2018
520 $aThe present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes.
650 $aMolecular biology
650 $aOocytes
650 $aBiologia Molecular
650 $aOvelha
653 $aOócito
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aARASHIRO, E. K. N.
700 1 $aCOSENTINO, I. O.
700 1 $aPINTO, P. H. N.
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tReproduction, Fertility and Development$gv. 30, n. 9, p. 1234-1244, 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 44 | |
| 2. |  | BARRETO, A. N.; LUZ, M. J. da S. e. Irrigação e drenagem 2 ed. rev. e amp. In: AZEVEDO, D. M. P. de; BELTRÃO, N. E. de M. O Agronegócio da mamona no Brasil. Campina Grande: Embrapa Algodão, 2007. p. 307-331| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
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| 3. | | BARRETO, A. N.; LUZ, M. J. da S. e. Irrigação e drenagem. In: SANTOS, R. C. dos; FREIRE, R. M. M.; SUASSUNA, T. de M. F. (Ed.). Amendoim: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa Informação Tecnológica: Campina Grande: Embrapa Algodão, 2009. p. 39-59| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Algodão. |
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| 19. | | BEZERRA, J. R. C.; LUZ, M. J. da S. e; SANTOS, J. W. dos; DIAS, J. M.; PEREIRA, J. R. Efeito de doses de nitrogênio sobre o rendimento do algodoeiro, cultivar BRS 201, em condições irrigadas. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Resumos... Uberlândia, 2007. p. 1-6 1 CD-ROM Solos e nutrição de plantas| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 44 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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